BBa_K2074000
1
BBa_K2074000
Cry11Aa+2A+Cyt2 expressed in C. reinhardtii
2016-10-16T11:00:00Z
2016-10-18T11:41:48Z
HSP70A-RBCS2 promoter cloned from Chlamydomonas reinhardtii. Cry11Aa and Cyt2 cloned from Bacillus thuringiensis serovar israelensis strain BRC-LLP29, codon optimal for Chlamydomonas reinhardtii. Extended FMDV 2A peptides cloned from FMDV, codon optimal for Chlamydomonas reinhardtii.
This devise is consisted of a HSP70A-RBCS2 promoter, Cry11Aa toxin, extended FMDV 2A peptides, a Cyt2 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
false
false
_2542_
30527
28088
9
false
none
false
Tong Tong
component2518600
1
BBa_K2074017
component2518601
1
BBa_K2074018
component2518603
1
BBa_K2074019
component2518602
1
BBa_K2074032
annotation2518603
1
BBa_K2074019
range2518603
1
3511
3744
annotation2518602
1
BBa_K2074032
range2518602
1
656
3502
annotation2518601
1
BBa_K2074018
range2518601
1
505
649
annotation2518600
1
BBa_K2074017
range2518600
1
1
496
BBa_K2074019
1
BBa_K2074019
Terminator of Chlamydomonas reinhardtii
2016-10-11T11:00:00Z
2016-10-16T06:09:16Z
The element is from the pCHlamy-3 vector
1
false
false
_2542_
30527
30527
9
false
3
false
Hao Feng
BBa_K2074017
1
BBa_K2074017
Hsp70A-Rbc S2 promoter
2016-10-10T11:00:00Z
2016-10-18T11:43:28Z
The element is from the pCHlamy-3 vector
1
false
false
_2542_
30527
30527
9
false
3
false
Hao Feng
BBa_K2074018
1
BBa_K2074018
Intron-1 Rbc S2
2016-10-10T11:00:00Z
2016-10-16T06:08:05Z
The element is from the pCHlamy-3 vector
1
false
false
_2542_
30527
30527
9
false
3
false
Hao Feng
BBa_K2074032
1
BBa_K2074032
Cry11Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)
2016-10-08T11:00:00Z
2016-10-16T03:20:23Z
DNA from a synthesis company.
cry10Aa and cyt2 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the toxicity to the larve of mosquito.
false
false
_2542_
30527
26735
9
false
To identify there is no restriction enzyme cutting site which we choosed.
false
Ruicheng Dai
BBa_K2074017_sequence
1
tcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaa
BBa_K2074019_sequence
1
ccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcg
BBa_K2074000_sequence
1
tcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaatactagaggtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcagtactagatgaactacatggaggacagcagcctggacaccctgagcatcgtgaacgagaccgacttccccctgtacaacaactacaccgagcccaccatcgcccccgccctgatcgccgtggcccccatcgcccagtacctggccaccgccatcggcaagtgggccgccaaggccgccttcagcaaggtgctgagcctgatcttccccggcagccagcccgccaccatggagaaggtgcgcaccgaggtggagaccctgatcaaccagaagctgagccaggaccgcgtgaacatcctgaacgccgagtaccgcggcatcatcgaggtgagcgacgtgttcgacgcctacatcaagcagcccggcttcacccccgccaccgccaagggctacttcctgaacctgagcggcgccatcatccagcgcctgccccagttcgaggtgcagacctacgagggcgtgtccatcgccctgttcacccagatgtgcaccctgcacctgaccctgctgaaggacggcatcctggccggcagcgcctggggcttcacgcaggccgacgtggactccttcatcaagctgttcaaccagaaggtgctggactaccgcacccgcctgatgcgcatgtacaccgaggagttcggccgcctgtgcaaggtgagcctgaaggacggcctgaccttccgcaacatgtgcaacctgtacgtgttccccttcgccgaggcctggagcctgatgcgctacgagggcctgaagctccagagcagcctgtccctgtgggactacgtgggcgtgagcatccccgtgaactacaacgagtggggcggcctggtgtacaagctgctgatgggcgaggtgaaccagcgcctgaccaccgtgaagttcaactactccttcaccaacgagcccgccgacatccccgcccgcgagaacatccgcggcgtgcaccccatctacgaccccagcagcggcctgaccggctggatcggcaacggccgcaccaacaacttcaacttcgccgacaacaacggcaacgagatcatggaggtgcgcacccagaccttctaccagaaccccaacaacgagcccatcgccccccgcgacatcatcaaccagatcctgaccgcccccgcccccgccgacctgttcttcaagaacgccgacatcaacgtgaagttcacccagtggttccagagcaccctgtacggctggaacatcaagctgggcacccagaccgtgctgagcagccgcaccggcaccatcccccccaactacctggcctacgacggctactacatccgcgccatcagcgcctgcccccgcggcgtgagcctggcctacaaccacgacctgaccaccctgacctacaaccgcatcgagtacgacagccccaccaccgagaacatcatcgtgggcttcgcccccgacaacaccaaggacttctacagcaagaagagccactacctgagcgagaccaacgacagctacgtgatccccgccctccagttcgccgaggtgagcgaccgcagcttcctggaggacacccccgaccaggccaccgacggcagcatcaagttcgcccgcaccttcatcagcaacgaggccaagtacagcatccgcctgaacaccggcttcaacaccgccacccgctacaagctgatcatccgcgtgcgcgtgccctaccgcctgcccgccggcatccgcgtgcagagccagaacagcggcaacaaccgcatgctgggcagcttcaccgccaacgccaaccccgagtgggtggacttcgtgaccgacgccttcaccttcaacgacctgggcatcaccaccagcagcaccaacgccctgttcagcatcagcagcgacagcctgaacagcggcgaggagtggtacctgagccagctgttcctggtgaaggagagcgcgttcacgacccagatcaaccccctgctgaagctgctggccatccaccccaccgaggcccgccacaagcagaagatcgtggcccccgtgaagcagaccctgaacttcgacctgctgaagctggccggcgacgtggagagcaaccccggccccatgcacctgaacaacctgaacaacttcaacaacctggagaacaacggcgagtaccactgctccgcccccatcatcaagaagcccttccgccacatcgccctgaccgtgcccagcagcgacatcaccaacttcaacgagatcttctacgtggagccccagtacatcgcccaggccatccgcctgaccaacaccttccagggcgccatcgaccccctgaccctgaacttcaacttcgagaaggccctccagatcgccaacggcctgcccaacgccggcgtgaccggcaccatcaaccagagcgtgatccaccagaccatcgaggtgagcgtgatgatcagccagatcaaggagatcatccgcagcgtgctgggcctggtgatcaacagcgccaacttctggaacagcgtggtgagcgccatcaccaacaccttcaccaacctggagccccaggtggacgagaactggatcgtgtggcgcaacctgagcgcgacccagaccagctacttctacaagatcctgttcagcatccagaacgaggacaccggccgcttcatggccatcctgcccatcgccttcgagatcaccgtggacgtgcagaagcagcagctgctgttcatcaccatcaaggacagcgcccgctacgaggtgaagatgaaggccctgaccgtggtgcaggccctggacagctacaacgcccccatcatcgacgtgttcaacgtgcgcaactacagcctgcaccgccccaaccacaacatcctccagaacctgaacgtgaaccccatcaagtgatactagagccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcg
BBa_K2074018_sequence
1
gtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcag
BBa_K2074032_sequence
1
atgaactacatggaggacagcagcctggacaccctgagcatcgtgaacgagaccgacttccccctgtacaacaactacaccgagcccaccatcgcccccgccctgatcgccgtggcccccatcgcccagtacctggccaccgccatcggcaagtgggccgccaaggccgccttcagcaaggtgctgagcctgatcttccccggcagccagcccgccaccatggagaaggtgcgcaccgaggtggagaccctgatcaaccagaagctgagccaggaccgcgtgaacatcctgaacgccgagtaccgcggcatcatcgaggtgagcgacgtgttcgacgcctacatcaagcagcccggcttcacccccgccaccgccaagggctacttcctgaacctgagcggcgccatcatccagcgcctgccccagttcgaggtgcagacctacgagggcgtgtccatcgccctgttcacccagatgtgcaccctgcacctgaccctgctgaaggacggcatcctggccggcagcgcctggggcttcacgcaggccgacgtggactccttcatcaagctgttcaaccagaaggtgctggactaccgcacccgcctgatgcgcatgtacaccgaggagttcggccgcctgtgcaaggtgagcctgaaggacggcctgaccttccgcaacatgtgcaacctgtacgtgttccccttcgccgaggcctggagcctgatgcgctacgagggcctgaagctccagagcagcctgtccctgtgggactacgtgggcgtgagcatccccgtgaactacaacgagtggggcggcctggtgtacaagctgctgatgggcgaggtgaaccagcgcctgaccaccgtgaagttcaactactccttcaccaacgagcccgccgacatccccgcccgcgagaacatccgcggcgtgcaccccatctacgaccccagcagcggcctgaccggctggatcggcaacggccgcaccaacaacttcaacttcgccgacaacaacggcaacgagatcatggaggtgcgcacccagaccttctaccagaaccccaacaacgagcccatcgccccccgcgacatcatcaaccagatcctgaccgcccccgcccccgccgacctgttcttcaagaacgccgacatcaacgtgaagttcacccagtggttccagagcaccctgtacggctggaacatcaagctgggcacccagaccgtgctgagcagccgcaccggcaccatcccccccaactacctggcctacgacggctactacatccgcgccatcagcgcctgcccccgcggcgtgagcctggcctacaaccacgacctgaccaccctgacctacaaccgcatcgagtacgacagccccaccaccgagaacatcatcgtgggcttcgcccccgacaacaccaaggacttctacagcaagaagagccactacctgagcgagaccaacgacagctacgtgatccccgccctccagttcgccgaggtgagcgaccgcagcttcctggaggacacccccgaccaggccaccgacggcagcatcaagttcgcccgcaccttcatcagcaacgaggccaagtacagcatccgcctgaacaccggcttcaacaccgccacccgctacaagctgatcatccgcgtgcgcgtgccctaccgcctgcccgccggcatccgcgtgcagagccagaacagcggcaacaaccgcatgctgggcagcttcaccgccaacgccaaccccgagtgggtggacttcgtgaccgacgccttcaccttcaacgacctgggcatcaccaccagcagcaccaacgccctgttcagcatcagcagcgacagcctgaacagcggcgaggagtggtacctgagccagctgttcctggtgaaggagagcgcgttcacgacccagatcaaccccctgctgaagctgctggccatccaccccaccgaggcccgccacaagcagaagatcgtggcccccgtgaagcagaccctgaacttcgacctgctgaagctggccggcgacgtggagagcaaccccggccccatgcacctgaacaacctgaacaacttcaacaacctggagaacaacggcgagtaccactgctccgcccccatcatcaagaagcccttccgccacatcgccctgaccgtgcccagcagcgacatcaccaacttcaacgagatcttctacgtggagccccagtacatcgcccaggccatccgcctgaccaacaccttccagggcgccatcgaccccctgaccctgaacttcaacttcgagaaggccctccagatcgccaacggcctgcccaacgccggcgtgaccggcaccatcaaccagagcgtgatccaccagaccatcgaggtgagcgtgatgatcagccagatcaaggagatcatccgcagcgtgctgggcctggtgatcaacagcgccaacttctggaacagcgtggtgagcgccatcaccaacaccttcaccaacctggagccccaggtggacgagaactggatcgtgtggcgcaacctgagcgcgacccagaccagctacttctacaagatcctgttcagcatccagaacgaggacaccggccgcttcatggccatcctgcccatcgccttcgagatcaccgtggacgtgcagaagcagcagctgctgttcatcaccatcaaggacagcgcccgctacgaggtgaagatgaaggccctgaccgtggtgcaggccctggacagctacaacgcccccatcatcgacgtgttcaacgtgcgcaactacagcctgcaccgccccaaccacaacatcctccagaacctgaacgtgaaccccatcaagtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z