BBa_K2074004 1 BBa_K2074004 Cry4Aa+2A+Cyt2 expressed in C. reinhardtii 2016-10-16T11:00:00Z 2016-10-18T02:52:47Z HSP70A-RBCS2 promoter cloned from Chlamydomonas reinhardtii. Cry4Aa and Cyt2 cloned from Bacillus thuringiensis serovar israelensis strain BRC-LLP29, codon optimal for Chlamydomonas reinhardtii. Extended FMDV 2A peptides cloned from FMDV, codon optimal for Chlamydomonas reinhardtii. This devise is consisted of a HSP70A-RBCS2 promoter, Cry4Aa toxin, extended FMDV 2A peptides, a Cyt2 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii. false false _2542_ 28088 28088 9 false none false Tong Tong component2518558 1 BBa_K2074018 component2518560 1 BBa_K2074019 component2518557 1 BBa_K2074017 component2518559 1 BBa_K2074028 annotation2518560 1 BBa_K2074019 range2518560 1 5074 5307 annotation2518557 1 BBa_K2074017 range2518557 1 1 496 annotation2518558 1 BBa_K2074018 range2518558 1 505 649 annotation2518559 1 BBa_K2074028 range2518559 1 656 5065 BBa_K2074019 1 BBa_K2074019 Terminator of Chlamydomonas reinhardtii 2016-10-11T11:00:00Z 2016-10-16T06:09:16Z The element is from the pCHlamy-3 vector 1 false false _2542_ 30527 30527 9 false 3 false Hao Feng BBa_K2074017 1 BBa_K2074017 Hsp70A-Rbc S2 promoter 2016-10-10T11:00:00Z 2016-10-18T11:43:28Z The element is from the pCHlamy-3 vector 1 false false _2542_ 30527 30527 9 false 3 false Hao Feng BBa_K2074018 1 BBa_K2074018 Intron-1 Rbc S2 2016-10-10T11:00:00Z 2016-10-16T06:08:05Z The element is from the pCHlamy-3 vector 1 false false _2542_ 30527 30527 9 false 3 false Hao Feng BBa_K2074028 1 BBa_K2074028 Cry4Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization) 2016-10-08T11:00:00Z 2016-10-18T04:53:42Z DNA from a synthesis company. cry4Aa and cyt2 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the toxicity to the larve of mosquito. false false _2542_ 30527 26735 9 true To identify there is no restriction enzyme cutting site which we choosed. false Ruicheng Dai BBa_K2074017_sequence 1 tcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaa BBa_K2074019_sequence 1 ccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcg BBa_K2074004_sequence 1 tcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaatactagaggtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcagtactagatgaacccctaccagaacaagaacgagtacgagaccctgaacgccagccagaagaagctgaacatcagcaacaactacacccgctaccccatcgagaacagccccaagcagctgctccagagcaccaactacaaggactggctgaacatgtgccagcagaaccagcagtacggcggcgacttcgagaccttcatcgacagcggcgagctgagcgcctacaccatcgtggtgggcaccgtgctgaccggcttcggcttcaccacccccctgggcctggccctgatcggcttcggcaccctgatccccgtgctgttccccgcccaggaccagagcaacacctggagcgacttcatcacccagaccaagaacatcatcaagaaggagatcgccagcacctacatcagcaacgccaacaagatcctgaaccgcagcttcaacgtgatcagcacctaccacaaccacctgaagacctgggagaacaaccccaacccccagaacacccaggacgtgcgcacccagatccagctggtgcactaccacttccagaacgtgatccccgagctggtgaacagctgcccccccaaccccagcgactgcgactactacaacatcctggtgctgagcagctacgcccaggccgccaacctgcacctgaccgtgctgaaccaggccgtgaagttcgaggcctacctgaagaacaaccgccagttcgactacctggagcccctgcccaccgccatcgactactaccccgtgctgaccaaggccatcgaggactacaccaactactgcgtgaccacctacaagaagggcctgaacctgatcaagaccacccccgacagcaacctggacggcaacatcaactggaacacctacaacacctaccgcaccaagatgaccaccgccgtgctggacctggtggccctgttccccaactacgacgtgggcaagtaccccatcggcgtgcagagcgagctgacccgcgagatctaccaggtgctgaacttcgaggagagcccctacaagtactacgacttccagtaccaggaggacagcctgacccgccgcccccacctcttcacctggctggacagcctgaacttctacgagaaggcccagaccacccccaacaacttcttcaccagccactacaacatgttccactacaccctggacaacatcagccagaagagcagcgtgttcggcaaccacaacgtgaccgacaagctgaagagcctgggcctggccaccaacatctacatcttcctgctgaacgtgatcagcctggacaacaagtacctgaacgactacaacaacatcagcaagatggacttcttcatcaccaacggcacccgcctgctggagaaggagctgaccgccggcagcggccagatcacctacgacgtgaacaagaacatcttcggcctgcccatcctgaagcgccgcgagaaccagggcaaccccaccctgttccccacctacgacaactacagccacatcctgagcttcatcaagagcctgagcatccccgccacctacaagacgcaggtgtacaccttcgcctggacccacagcagcgtggaccccaagaacaccatctacacccacctgaccacccagatccccgccgtgaaggccaacagcctgggcacggccagcaaggtggtgcagggccccggccacaccggcggcgacctgatcgacttcaaggaccacttcaagatcacctgccagcacagcaacttccagcagagctacttcatccgcatccgctacgccagcaacggcagcgccaacacccgcgccgtgatcaacctgagcatccccggcgtggccgagctgggcatggccctgaaccccaccttcagcggcaccgactacaccaacctgaagtacaaggacttccagtacctggagttcagcaacgaggtgaagttcgcccccaaccagaacatcagcctcgtgttcaaccgcagcgacgtgtacaccaacaccaccgtgctgatcgacaagatcgagttcctgcccatcacccgcagcatccgcgaggaccgcgagaagcagaagctggagaccgtgcagcagatcatcaacaccttctacgccaaccccatcaagaacaccctccagagcgagctgaccgactacgacatcgaccaggccgccaacctcgtggagtgcatcagcgaggagctgtaccccaaggagaagatgctgctgctggacgaggtgaagaacgccaagcagctgagccagagccgcaacgtgctccagaacggcgacttcgagagcgccaccctcggctggaccaccagcgacaacatcaccatccaggaggacgaccccatcttcaagggccactacctgcacatgagcggcgcccgcgacatcgacggcaccatcttccccacctacatcttccagaagatcgacgagagcaagctgaagccctacacccgctacctggtgcgcggcttcgtgggcagcagcaaggacgtggagctggtggtgagccgctacggcgaggagatcgacgccatcatgaacgtgcccgccgacctgaactacctgtaccccagcaccttcgactgcgagggcagcaaccgctgcgagaccagcgccgtgcccgccaacatcggcaacaccagcgacatgctgtacagctgccagtacgacaccggcaagaagcacgtggtgtgccaggacagccaccagttcagcttcaccatcgacaccggcgccctggacaccaacgagaacatcggcgtgtgggtgatgttcaagatcagcagccccgacggctacgccagcctggacaacctggaggtgatcgaggagggccccatcgacggcgaggccctgagccgcgtgaagcacatggagaagaagtggaacgaccagatggaggccaagcgcagcgagacccagcaggcctacgacgtggccaagcaggccatcgacgccctgttcaccaacgtgcaggacgaggccctccagttcgacaccaccctggcccagatccagtacgccgagtacctggtgcagagcatcccctacgtgtacaacgactggctgagcgacgtgcccggcatgaactacgacatctacgtggagctggacgcgcgcgtggcccaggcccgctacctgtacgacacccgcaacatcatcaagaacggcgacttcacccagggcgtgatgggctggcacgtgaccggcaacgccgacgtgcagcagatcgacggcgtgagcgtgctggtgctgagcaactggagcgcgggcgtgagccagaacgtgcacctccagcacaaccacggctacgtgctgcgcgtgatcgccaagaaggagggccccggcaacggctacgtgaccctgatggactgcgaggagaaccaggagaagctgaccttcaccagctgcgaggagggctacatcaccaagaccgtggacgtgttccccgacaccgaccgcgtgcgcatcgagatcggcgagaccgagggcagcttctacatcgagagcatcgagctcatctgcatgaacgagctgctggccatccaccccaccgaggcccgccacaagcagaagatcgtggcccccgtgaagcagaccctgaacttcgacctgctgaagctggccggcgacgtggagagcaaccccggccccatggagaacctgaaccactgccccctggaggacatcaaggtgaacccctggaagaccccccagagcaaggcccgcgtgatcaccctgcgcgtggaggaccccaacgagatcaacaacctgctgagcatcaacgagatcgacaaccccaactacatcctccaggccatcatgctggccaacgccttccagaacgccctggtgcccaccagcaccgacttcggcgacgccctgcgcttcagcatgcccaagggcctggagatcgccaacaccatcacccccatgggcgccgtggtgagctacgtggaccagaacgtgacccagaccaacaaccaggtgagcgtgatgatcaacaaggtgctggaggtgctgaagaccgtgctgggcgtggccctgagcggcagcgtgatcgaccagctgaccgccgccgtgaccaacaccttcaccaacctgaacacccagaagaacgaggcctggatcttctggggcaaggagaccgccaaccagaccaactacacctacaacgtgctgttcgccatccagaacgcccagaccggcggcgtgatgtactgcgtgcccgtgggcttcgagatcaaggtgagcgccgtgaaggagcaggtgctgttcttcaccatccaggactccgccagctacaacgtgaacatccagagcctgaagttcgcgcagcccctggtgagcagcagccagtaccccatcgccgacctgaccagcgccatcaacggcaccctgtaatactagagccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcg BBa_K2074018_sequence 1 gtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcag BBa_K2074028_sequence 1 atgaacccctaccagaacaagaacgagtacgagaccctgaacgccagccagaagaagctgaacatcagcaacaactacacccgctaccccatcgagaacagccccaagcagctgctccagagcaccaactacaaggactggctgaacatgtgccagcagaaccagcagtacggcggcgacttcgagaccttcatcgacagcggcgagctgagcgcctacaccatcgtggtgggcaccgtgctgaccggcttcggcttcaccacccccctgggcctggccctgatcggcttcggcaccctgatccccgtgctgttccccgcccaggaccagagcaacacctggagcgacttcatcacccagaccaagaacatcatcaagaaggagatcgccagcacctacatcagcaacgccaacaagatcctgaaccgcagcttcaacgtgatcagcacctaccacaaccacctgaagacctgggagaacaaccccaacccccagaacacccaggacgtgcgcacccagatccagctggtgcactaccacttccagaacgtgatccccgagctggtgaacagctgcccccccaaccccagcgactgcgactactacaacatcctggtgctgagcagctacgcccaggccgccaacctgcacctgaccgtgctgaaccaggccgtgaagttcgaggcctacctgaagaacaaccgccagttcgactacctggagcccctgcccaccgccatcgactactaccccgtgctgaccaaggccatcgaggactacaccaactactgcgtgaccacctacaagaagggcctgaacctgatcaagaccacccccgacagcaacctggacggcaacatcaactggaacacctacaacacctaccgcaccaagatgaccaccgccgtgctggacctggtggccctgttccccaactacgacgtgggcaagtaccccatcggcgtgcagagcgagctgacccgcgagatctaccaggtgctgaacttcgaggagagcccctacaagtactacgacttccagtaccaggaggacagcctgacccgccgcccccacctcttcacctggctggacagcctgaacttctacgagaaggcccagaccacccccaacaacttcttcaccagccactacaacatgttccactacaccctggacaacatcagccagaagagcagcgtgttcggcaaccacaacgtgaccgacaagctgaagagcctgggcctggccaccaacatctacatcttcctgctgaacgtgatcagcctggacaacaagtacctgaacgactacaacaacatcagcaagatggacttcttcatcaccaacggcacccgcctgctggagaaggagctgaccgccggcagcggccagatcacctacgacgtgaacaagaacatcttcggcctgcccatcctgaagcgccgcgagaaccagggcaaccccaccctgttccccacctacgacaactacagccacatcctgagcttcatcaagagcctgagcatccccgccacctacaagacgcaggtgtacaccttcgcctggacccacagcagcgtggaccccaagaacaccatctacacccacctgaccacccagatccccgccgtgaaggccaacagcctgggcacggccagcaaggtggtgcagggccccggccacaccggcggcgacctgatcgacttcaaggaccacttcaagatcacctgccagcacagcaacttccagcagagctacttcatccgcatccgctacgccagcaacggcagcgccaacacccgcgccgtgatcaacctgagcatccccggcgtggccgagctgggcatggccctgaaccccaccttcagcggcaccgactacaccaacctgaagtacaaggacttccagtacctggagttcagcaacgaggtgaagttcgcccccaaccagaacatcagcctcgtgttcaaccgcagcgacgtgtacaccaacaccaccgtgctgatcgacaagatcgagttcctgcccatcacccgcagcatccgcgaggaccgcgagaagcagaagctggagaccgtgcagcagatcatcaacaccttctacgccaaccccatcaagaacaccctccagagcgagctgaccgactacgacatcgaccaggccgccaacctcgtggagtgcatcagcgaggagctgtaccccaaggagaagatgctgctgctggacgaggtgaagaacgccaagcagctgagccagagccgcaacgtgctccagaacggcgacttcgagagcgccaccctcggctggaccaccagcgacaacatcaccatccaggaggacgaccccatcttcaagggccactacctgcacatgagcggcgcccgcgacatcgacggcaccatcttccccacctacatcttccagaagatcgacgagagcaagctgaagccctacacccgctacctggtgcgcggcttcgtgggcagcagcaaggacgtggagctggtggtgagccgctacggcgaggagatcgacgccatcatgaacgtgcccgccgacctgaactacctgtaccccagcaccttcgactgcgagggcagcaaccgctgcgagaccagcgccgtgcccgccaacatcggcaacaccagcgacatgctgtacagctgccagtacgacaccggcaagaagcacgtggtgtgccaggacagccaccagttcagcttcaccatcgacaccggcgccctggacaccaacgagaacatcggcgtgtgggtgatgttcaagatcagcagccccgacggctacgccagcctggacaacctggaggtgatcgaggagggccccatcgacggcgaggccctgagccgcgtgaagcacatggagaagaagtggaacgaccagatggaggccaagcgcagcgagacccagcaggcctacgacgtggccaagcaggccatcgacgccctgttcaccaacgtgcaggacgaggccctccagttcgacaccaccctggcccagatccagtacgccgagtacctggtgcagagcatcccctacgtgtacaacgactggctgagcgacgtgcccggcatgaactacgacatctacgtggagctggacgcgcgcgtggcccaggcccgctacctgtacgacacccgcaacatcatcaagaacggcgacttcacccagggcgtgatgggctggcacgtgaccggcaacgccgacgtgcagcagatcgacggcgtgagcgtgctggtgctgagcaactggagcgcgggcgtgagccagaacgtgcacctccagcacaaccacggctacgtgctgcgcgtgatcgccaagaaggagggccccggcaacggctacgtgaccctgatggactgcgaggagaaccaggagaagctgaccttcaccagctgcgaggagggctacatcaccaagaccgtggacgtgttccccgacaccgaccgcgtgcgcatcgagatcggcgagaccgagggcagcttctacatcgagagcatcgagctcatctgcatgaacgagctgctggccatccaccccaccgaggcccgccacaagcagaagatcgtggcccccgtgaagcagaccctgaacttcgacctgctgaagctggccggcgacgtggagagcaaccccggccccatggagaacctgaaccactgccccctggaggacatcaaggtgaacccctggaagaccccccagagcaaggcccgcgtgatcaccctgcgcgtggaggaccccaacgagatcaacaacctgctgagcatcaacgagatcgacaaccccaactacatcctccaggccatcatgctggccaacgccttccagaacgccctggtgcccaccagcaccgacttcggcgacgccctgcgcttcagcatgcccaagggcctggagatcgccaacaccatcacccccatgggcgccgtggtgagctacgtggaccagaacgtgacccagaccaacaaccaggtgagcgtgatgatcaacaaggtgctggaggtgctgaagaccgtgctgggcgtggccctgagcggcagcgtgatcgaccagctgaccgccgccgtgaccaacaccttcaccaacctgaacacccagaagaacgaggcctggatcttctggggcaaggagaccgccaaccagaccaactacacctacaacgtgctgttcgccatccagaacgcccagaccggcggcgtgatgtactgcgtgcccgtgggcttcgagatcaaggtgagcgccgtgaaggagcaggtgctgttcttcaccatccaggactccgccagctacaacgtgaacatccagagcctgaagttcgcgcagcccctggtgagcagcagccagtaccccatcgccgacctgaccagcgccatcaacggcaccctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z