BBa_K2074019
1
BBa_K2074019
Terminator of Chlamydomonas reinhardtii
2016-10-11T11:00:00Z
2016-10-16T06:09:16Z
The element is from the pCHlamy-3 vector
1
false
false
_2542_
30527
30527
9
false
3
false
Hao Feng
BBa_K2074030
1
BBa_K2074030
Cry10Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)
2016-10-08T11:00:00Z
2016-10-16T03:19:57Z
DNA from a synthesis company.
cry10Aa and cyt2 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the toxicity to the larve of mosquito.
false
false
_2542_
30527
26735
9
false
To identify there is no restriction enzyme cutting site which we choosed.
false
Ruicheng Dai
BBa_K2074006
1
BBa_K2074006
Cry10Aa+2A+Cyt2 expressed in C. reinhardtii
2016-10-16T11:00:00Z
2016-10-18T02:55:45Z
HSP70A-RBCS2 promoter cloned from Chlamydomonas reinhardtii. Cry10Aa and Cyt2 cloned from Bacillus thuringiensis serovar israelensis strain BRC-LLP29, codon optimal for Chlamydomonas reinhardtii. Extended FMDV 2A peptides cloned from FMDV, codon optimal for Chlamydomonas reinhardtii.
This devise is consisted of a HSP70A-RBCS2 promoter, Cry10Aa toxin, extended FMDV 2A peptides, a Cyt2 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
false
false
_2542_
28088
28088
9
false
none
false
Tong Tong
component2518593
1
BBa_K2074018
component2518594
1
BBa_K2074030
component2518595
1
BBa_K2074019
component2518592
1
BBa_K2074017
annotation2518595
1
BBa_K2074019
range2518595
1
3598
3831
annotation2518592
1
BBa_K2074017
range2518592
1
1
496
annotation2518593
1
BBa_K2074018
range2518593
1
505
649
annotation2518594
1
BBa_K2074030
range2518594
1
656
3589
BBa_K2074017
1
BBa_K2074017
Hsp70A-Rbc S2 promoter
2016-10-10T11:00:00Z
2016-10-18T11:43:28Z
The element is from the pCHlamy-3 vector
1
false
false
_2542_
30527
30527
9
false
3
false
Hao Feng
BBa_K2074018
1
BBa_K2074018
Intron-1 Rbc S2
2016-10-10T11:00:00Z
2016-10-16T06:08:05Z
The element is from the pCHlamy-3 vector
1
false
false
_2542_
30527
30527
9
false
3
false
Hao Feng
BBa_K2074017_sequence
1
tcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaa
BBa_K2074019_sequence
1
ccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcg
BBa_K2074018_sequence
1
gtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcag
BBa_K2074006_sequence
1
tcgctgaggcttgacatgattggtgcgtatgtttgtatgaagctacaggactgatttggcgggctatgagggcgggggaagctctggaagggccgcgatggggcgcgcggcgtccagaaggcgccatacggcccgctggcggcacccatccggtataaaagcccgcgaccccgaacggtgacctccactttcagcgacaaacgagcacttatacatacgcgactattctgccgctatacataaccactcagctagcttaagatcccatcaagcttgcatgccgggcgcgccagaaggagcgcagccaaaccaggatgatgtttgatggggtatttgagcacttgcaacccttatccggaagccccctggcccacaaaggctaggcgccaatgcaagcagttcgcatgcagcccctggagcggtgccctcctgataaaccggccagggggcctatgttctttacttttttacaagagaagtcactcaacatcttaaatactagaggtgagtcgacgagcaagcccggcggatcaggcagcgtgcttgcagatttgacttgcaacgcccgcattgtgtcgacgaaggcttttggctcctctgtcgctgtctcaagcagcatctaaccctgcgtcgccgtttccatttgcagtactagatgaacccctaccagaacaagaacgagtacgagatcttcaacgcccccagcaacggcttcagcaagagcaacaactacagccgctaccccctggccaacaagcccaaccagcccctgaagaacaccaactacaaggactggctgaacgtgtgccaggacaaccagcagtacggcaacaacgccggcaacttcgccagcagcgagaccatcgtgggcgtgagcgccggcatcatcgtggtgggcaccatgctgggcgccttcgccgcccccgtgctggccgccggcatcatcagcttcggcacgctgctgcccatcttctggcagggcagcgaccccgccaacgtgtggcaggacctgctgaacatcggcggccgccccatccaggagatcgacaagaacatcatcaacgtgctgaccagcatcgtgacccccatcaagaaccagctggacaagtaccaggagttcttcgacaagtgggagcccgcccgcacccacgccaacgccaaggccgtgcacgacctgttcaccaccctggagcccatcatcgacaaggacctggacatgctgaagaacaacgccagctaccgcatccccaccctgcccgcctacgcccagatcgccacctggcacctgaacctgctgaagcacgccgccacctactacaacatctggctccagaaccagggcatcaaccccagcaccttcaacagcagcaactactaccagggctacctgaagcgcaagatccaggagtacaccgactactgcatccagacctacaacgccggcctgaccatgatccgcaccaacaccaacgccacctggaacatgtacaacacctaccgcctggagatgaccctgaccgtgctggacctgatcgccatcttccccaactacgaccccgagaagtaccccatcggcgtgaagagcgagctgatccgcgaggtgtacaccaacgtgaacagcgacaccttccgcaccatcaccgagctggagaacggcctgacccgcaaccccaccctgttcacctggatcaaccagggccgcttctacacccgcaacagccgcgacatcctggacccctacgacatcttcagcttcaccggcaaccagatggccttcacccacaccaacgacgaccgcaacatcatctggggcgccgtgcacggcaacatcatcagccaggacacgagcaaggtgttccccttctaccgcaacaagcccatcgacaaggtggagatcgtgcgccaccgcgagtacagcgacatcatctacgagatgatcttcttcagcaacagcagcgaggtgttccgctacagcagcaacagcaccatcgagaacaactacaagcgcaccgacagctacatgatccccaagcagacctggaagaacaaggagtacggccacaccctgagctacatcaagaccgacaactacatcttcagcgtggtgcgcgagcgccggcgcgtggccttcagctggacccacaccagcgtggacttccagaacaccatcgacctggacaacatcacccagatccacgccctgaaggccctgaaggtgagcagcgacagcaagatcgtgaagggccccggccacaccggcggcgacctggtgatcctgaaggacagcatggacttccgcgtgcgcttcctgaagaacgtgagccgccagtaccaggtgcgcatccgctacgccaccaacgcccccaagaccaccgtgttcctgaccggcatcgacaccatcagcgtggagctgcccagcaccaccagccgccagaaccccaacgccaccgacctgacctacgccgacttcggctacgtgaccttcccccgcaccgtgcccaacaagaccttcgagggcgaggacaccctgctgatgaccctgtacggcacccccaaccacagctacaacatctacatcgacaagatcgagttcatccccatcacccagagcgtgctggactacaccgagaagcagaacatcgagaagacccagaagatcgtgaacgacctgttcgtgaacctgctggccatccaccccaccgaggcccgccacaagcagaagatcgtggcccccgtgaagcagaccctgaacttcgacctgctgaagctggccggcgacgtggagagcaaccccggccccatgcacctgaacaacctgaacaacttcaacaacctggagaacaacggcgagtaccactgctccgcccccatcatcaagaagcccttccgccacatcgccctgaccgtgcccagcagcgacatcaccaacttcaacgagatcttctacgtggagccccagtacatcgcccaggccatccgcctgaccaacaccttccagggcgccatcgaccccctgaccctgaacttcaacttcgagaaggccctccagatcgccaacggcctgcccaacgccggcgtgaccggcaccatcaaccagagcgtgatccaccagaccatcgaggtgagcgtgatgatcagccagatcaaggagatcatccgcagcgtgctgggcctggtgatcaacagcgccaacttctggaacagcgtggtgagcgccatcaccaacaccttcaccaacctggagccccaggtggacgagaactggatcgtgtggcgcaacctgagcgcgacccagaccagctacttctacaagatcctgttcagcatccagaacgaggacaccggccgcttcatggccatcctgcccatcgccttcgagatcaccgtggacgtgcagaagcagcagctgctgttcatcaccatcaaggacagcgcccgctacgaggtgaagatgaaggccctgaccgtggtgcaggccctggacagctacaacgcccccatcatcgacgtgttcaacgtgcgcaactacagcctgcaccgccccaaccacaacatcctccagaacctgaacgtgaaccccatcaagtgatactagagccgctccgtgtaaatggaggcgctcgttgatctgagccttgccccctgacgaacggcggtggatggaagatactgctctcaagtgctgaagcggtagcttagctccccgtttcgtgctgatcagtctttttcaacacgtaaaaagcggaggagttttgcaattttgttggttgtaacgatcctccgttgattttggcctctttctccatgggcgggctgggcgtatttgaagcg
BBa_K2074030_sequence
1
atgaacccctaccagaacaagaacgagtacgagatcttcaacgcccccagcaacggcttcagcaagagcaacaactacagccgctaccccctggccaacaagcccaaccagcccctgaagaacaccaactacaaggactggctgaacgtgtgccaggacaaccagcagtacggcaacaacgccggcaacttcgccagcagcgagaccatcgtgggcgtgagcgccggcatcatcgtggtgggcaccatgctgggcgccttcgccgcccccgtgctggccgccggcatcatcagcttcggcacgctgctgcccatcttctggcagggcagcgaccccgccaacgtgtggcaggacctgctgaacatcggcggccgccccatccaggagatcgacaagaacatcatcaacgtgctgaccagcatcgtgacccccatcaagaaccagctggacaagtaccaggagttcttcgacaagtgggagcccgcccgcacccacgccaacgccaaggccgtgcacgacctgttcaccaccctggagcccatcatcgacaaggacctggacatgctgaagaacaacgccagctaccgcatccccaccctgcccgcctacgcccagatcgccacctggcacctgaacctgctgaagcacgccgccacctactacaacatctggctccagaaccagggcatcaaccccagcaccttcaacagcagcaactactaccagggctacctgaagcgcaagatccaggagtacaccgactactgcatccagacctacaacgccggcctgaccatgatccgcaccaacaccaacgccacctggaacatgtacaacacctaccgcctggagatgaccctgaccgtgctggacctgatcgccatcttccccaactacgaccccgagaagtaccccatcggcgtgaagagcgagctgatccgcgaggtgtacaccaacgtgaacagcgacaccttccgcaccatcaccgagctggagaacggcctgacccgcaaccccaccctgttcacctggatcaaccagggccgcttctacacccgcaacagccgcgacatcctggacccctacgacatcttcagcttcaccggcaaccagatggccttcacccacaccaacgacgaccgcaacatcatctggggcgccgtgcacggcaacatcatcagccaggacacgagcaaggtgttccccttctaccgcaacaagcccatcgacaaggtggagatcgtgcgccaccgcgagtacagcgacatcatctacgagatgatcttcttcagcaacagcagcgaggtgttccgctacagcagcaacagcaccatcgagaacaactacaagcgcaccgacagctacatgatccccaagcagacctggaagaacaaggagtacggccacaccctgagctacatcaagaccgacaactacatcttcagcgtggtgcgcgagcgccggcgcgtggccttcagctggacccacaccagcgtggacttccagaacaccatcgacctggacaacatcacccagatccacgccctgaaggccctgaaggtgagcagcgacagcaagatcgtgaagggccccggccacaccggcggcgacctggtgatcctgaaggacagcatggacttccgcgtgcgcttcctgaagaacgtgagccgccagtaccaggtgcgcatccgctacgccaccaacgcccccaagaccaccgtgttcctgaccggcatcgacaccatcagcgtggagctgcccagcaccaccagccgccagaaccccaacgccaccgacctgacctacgccgacttcggctacgtgaccttcccccgcaccgtgcccaacaagaccttcgagggcgaggacaccctgctgatgaccctgtacggcacccccaaccacagctacaacatctacatcgacaagatcgagttcatccccatcacccagagcgtgctggactacaccgagaagcagaacatcgagaagacccagaagatcgtgaacgacctgttcgtgaacctgctggccatccaccccaccgaggcccgccacaagcagaagatcgtggcccccgtgaagcagaccctgaacttcgacctgctgaagctggccggcgacgtggagagcaaccccggccccatgcacctgaacaacctgaacaacttcaacaacctggagaacaacggcgagtaccactgctccgcccccatcatcaagaagcccttccgccacatcgccctgaccgtgcccagcagcgacatcaccaacttcaacgagatcttctacgtggagccccagtacatcgcccaggccatccgcctgaccaacaccttccagggcgccatcgaccccctgaccctgaacttcaacttcgagaaggccctccagatcgccaacggcctgcccaacgccggcgtgaccggcaccatcaaccagagcgtgatccaccagaccatcgaggtgagcgtgatgatcagccagatcaaggagatcatccgcagcgtgctgggcctggtgatcaacagcgccaacttctggaacagcgtggtgagcgccatcaccaacaccttcaccaacctggagccccaggtggacgagaactggatcgtgtggcgcaacctgagcgcgacccagaccagctacttctacaagatcctgttcagcatccagaacgaggacaccggccgcttcatggccatcctgcccatcgccttcgagatcaccgtggacgtgcagaagcagcagctgctgttcatcaccatcaaggacagcgcccgctacgaggtgaagatgaaggccctgaccgtggtgcaggccctggacagctacaacgcccccatcatcgacgtgttcaacgtgcgcaactacagcctgcaccgccccaaccacaacatcctccagaacctgaacgtgaaccccatcaagtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z