BBa_K2074034 1 BBa_K2074034 Cry11Aa(Codon optimization)+2A+EGFP(Codon optimization) 2016-10-08T11:00:00Z 2016-10-22T12:42:03Z DNA from a synthesis company. we use 2A system to link cry11Aa and EGFP then transferred the gene into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein . false false _2542_ 30527 26735 9 true To identify there is no restriction enzyme cutting site which we choosed. false Ruicheng Dai BBa_K2074034_sequence 1 atgaactacatggaggacagcagcctggacaccctgagcatcgtgaacgagaccgacttccccctgtacaacaactacaccgagcccaccatcgcccccgccctgatcgccgtggcccccatcgcccagtacctggccaccgccatcggcaagtgggccgccaaggccgccttcagcaaggtgctgagcctgatcttccccggcagccagcccgccaccatggagaaggtgcgcaccgaggtggagaccctgatcaaccagaagctgagccaggaccgcgtgaacatcctgaacgccgagtaccgcggcatcatcgaggtgagcgacgtgttcgacgcctacatcaagcagcccggcttcacccccgccaccgccaagggctacttcctgaacctgagcggcgccatcatccagcgcctgccccagttcgaggtgcagacctacgagggcgtgtccatcgccctgttcacccagatgtgcaccctgcacctgaccctgctgaaggacggcatcctggccggcagcgcctggggcttcacgcaggccgacgtggactccttcatcaagctgttcaaccagaaggtgctggactaccgcacccgcctgatgcgcatgtacaccgaggagttcggccgcctgtgcaaggtgagcctgaaggacggcctgaccttccgcaacatgtgcaacctgtacgtgttccccttcgccgaggcctggagcctgatgcgctacgagggcctgaagctccagagcagcctgtccctgtgggactacgtgggcgtgagcatccccgtgaactacaacgagtggggcggcctggtgtacaagctgctgatgggcgaggtgaaccagcgcctgaccaccgtgaagttcaactactccttcaccaacgagcccgccgacatccccgcccgcgagaacatccgcggcgtgcaccccatctacgaccccagcagcggcctgaccggctggatcggcaacggccgcaccaacaacttcaacttcgccgacaacaacggcaacgagatcatggaggtgcgcacccagaccttctaccagaaccccaacaacgagcccatcgccccccgcgacatcatcaaccagatcctgaccgcccccgcccccgccgacctgttcttcaagaacgccgacatcaacgtgaagttcacccagtggttccagagcaccctgtacggctggaacatcaagctgggcacccagaccgtgctgagcagccgcaccggcaccatcccccccaactacctggcctacgacggctactacatccgcgccatcagcgcctgcccccgcggcgtgagcctggcctacaaccacgacctgaccaccctgacctacaaccgcatcgagtacgacagccccaccaccgagaacatcatcgtgggcttcgcccccgacaacaccaaggacttctacagcaagaagagccactacctgagcgagaccaacgacagctacgtgatccccgccctccagttcgccgaggtgagcgaccgcagcttcctggaggacacccccgaccaggccaccgacggcagcatcaagttcgcccgcaccttcatcagcaacgaggccaagtacagcatccgcctgaacaccggcttcaacaccgccacccgctacaagctgatcatccgcgtgcgcgtgccctaccgcctgcccgccggcatccgcgtgcagagccagaacagcggcaacaaccgcatgctgggcagcttcaccgccaacgccaaccccgagtgggtggacttcgtgaccgacgccttcaccttcaacgacctgggcatcaccaccagcagcaccaacgccctgttcagcatcagcagcgacagcctgaacagcggcgaggagtggtacctgagccagctgttcctggtgaaggagagcgcgttcacgacccagatcaaccccctgctgaagctgctggccatccaccccaccgaggcccgccacaagcagaagatcgtggcccccgtgaagcagaccctgaacttcgacctgctgaagctggccggcgacgtggagagcaaccccggccccatggtgagcaagggcgaggagctgttcaccggcgtggtgcccatcctggtggagctggacggcgacgtgaacggccacaagttcagcgtgagcggcgagggcgagggcgacgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctggtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagagcgccatgcccgagggctacgtgcaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgggccacaagctggagtacaactacaacagccacaacgtgtacatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctggccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagagcgccctgagcaaggaccccaacgagaagcgcgaccacatggtgctgctggagttcgtgaccgccgccggcatcaccctgggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z