LOCUS BBa_K2075004 3849 bp DNA linear 25-Nov-2024 DEFINITION Vector iGem_02_Ax7L- DS ACCESSION BBa_K2075004 VERSION BBa_K2075004.1 FEATURES Location/Qualifiers CDS 1..117 /label=Npu DnaE(C) peptide CDS 118..162 /label=Linker N-Term CDS 1..162 /label=BBa_K2075010 CDS 169..3378 /label=Ax7L-DS CDS 169..3378 /label=BBa_K2075020 misc_feature 3387..3407 /label=TEV Site misc_feature 3387..3407 /label=BBa_K2075003 misc_feature 3416..3505 /label=Strep TX Tag misc_feature 3416..3505 /label=BBa_K2075000 CDS 3514..3540 /label=Intein linker (C-term) CDS 3541..3849 /label=Intein (C-term) CDS 3514..3849 /label=BBa_K2075024 ORIGIN 1 atgatcaaaa tagccacacg taaatattta ggcaaacaaa atgtctatga cattggagtt 61 gagcgcgacc ataattttgc actcaaaaat ggcttcatag cttccaactg tttcaatggc 121 ggttcgggcg gttcgggcgg cacaggcggt tcgggcggtt cttactagat gatcaaaata 181 gccacacgta aatatttagg caaacaaaat gtctatgaca ttggagttga gcgcgaccat 241 aattttgcac tcaaaaatgg cttcatagct tccaactgtt tcaatggcgg ttcgggcggt 301 tcgggcggca caggcggttc gggcggttct atgtacccat acgatgttcc tgactatgcg 361 gccaagaaga agaggaaggt gcaggtggat ctacgcacgc tcggctacag ccagcagcaa 421 caggagaaga tcaaaccgaa ggttcgttcg acagtggcgc agcaccacga ggcactggtc 481 ggccatgggt ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg 541 accgtcgctg tcaagtatca ggacatgatc gcagcgttgc cagaggcgac acacgaagcg 601 atcgttggcg tcggcaaaca gtggtccggc gcacgcgctc tggaggcctt gctcacggtg 661 gcgggagagt tgagaggtcc accgttacag ttggacacag gccaacttct caagattgca 721 aagcgtggcg gcgtgaccgc agtggaggca gtgcatgcat ggcgcaatgc actgacgggt 781 gcccccctga accttacccc ggagcaggtg gtggccatcg ccagcaatat tggtggcaag 841 caggcgctgg agacggtgca gcggctgttg ccggtgctgt gccaggccca tggcctgacc 901 ccggagcagg tggtggccat cgccagcaat aacggcggca agcaggcgct ggagacggtg 961 cagcggctgt tgccggtgct gtgccaggcc catggcctga caccggagca ggtggtggcc 1021 atcgccagcc acgatggtgg caagcaggcg ctggagacgg tgcagcggct gttgccggtg 1081 ctgtgccagg cccatggcct caccccggag caggtggtgg ccatcgccag caatattggc 1141 ggcaagcagg cgctggagac ggtgcagcgg ctgttgccgg tgctgtgcca ggcccatggc 1201 ctgactccgg agcaggtggt ggccatcgcc agccacgatg gcggcaagca ggcgctggag 1261 acggtgcagc ggctgttgcc ggtgctgtgc caggcccatg gcctgacccc ggagcaggtg 1321 gtggccatcg ccagcaatgg cggcggcaag caggcgctgg agacggtgca gcggctgttg 1381 ccggtgctgt gccaggcgca tggccttacc ccggagcagg tggtggccat cgccagcaat 1441 attggtggca agcaggcgct ggagacggtg cagcggctgt tgccggtgct gtgccaggcc 1501 catggcctga ccccggagca ggtggtggcc atcgccagca atggcggcgg caagcaggcg 1561 ctggagacgg tgcagcggct gttgccggtg ctgtgccagg cccatggcct gacaccggag 1621 caggtggtgg ccatcgccag caatattggt ggcaagcagg cgctggagac ggtgcagcgg 1681 ctgttgccgg tgctgtgcca ggcccatggc ctcaccccgg agcaggtggt ggccatcgcc 1741 agcaatggcg gcggcaagca ggcgctggag acggtgcagc ggctgttgcc ggtgctgtgc 1801 caggcccatg gcctgactcc ggagcaggtg gtggccatcg ccagcaatat tggcggcaag 1861 caggcgctgg agacggtgca gcggctgttg ccggtgctgt gccaggccca tggcctgacc 1921 ccggagcagg tggtggccat cgccagcaat attggcggca agcaggcgct ggagacggtg 1981 cagcggctgt tgccggtgct gtgccaggcg catggcctta ccccggagca ggtggtggcc 2041 atcgccagca atattggtgg caagcaggcg ctggagacgg tgcagcggct gttgccggtg 2101 ctgtgccagg cccatggcct gaccccggag caggtggtgg ccatcgccag ccacgatggc 2161 ggcaagcagg cgctggagac ggtgcagcgg ctgttgccgg tgctgtgcca ggcccatggc 2221 ctgacaccgg agcaggtggt ggccatcgcc agccacgatg gtggcaagca ggcgctggag 2281 acggtgcagc ggctgttgcc ggtgctgtgc caggcccatg gcctcacccc ggagcaggtg 2341 gtggccatcg ccagccacga tggcggcaag caggcgctgg agacggtgca gcggctgttg 2401 ccggtgctgt gccaggccca tggcctgact ccggagcagg tggtggccat cgccagccac 2461 gatggcggca agcaggcgct ggagacggtg cagcggctgt tgccggtgct gtgccaggcc 2521 catggcctga ccccggagca ggtggtggcc atcgccagcc acgatggcgg caagcaggcg 2581 ctggagagca ttgttgccca gttatctcgc cctgatccgt cgttggccgc gttaaccaac 2641 gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg cgctggatgc agtgaaaaag 2701 ggattgccgc acgcgccggc cttgatcaaa agaaccaatc gccgtattcc cgaacgcaca 2761 tcccatcgcg ttgccggatc ccagctggtg aagagcgagc tggaggagaa gaagtccgag 2821 ctgcggcaca agctgaagta cgtgccccac gagtacatcg agctgatcga gatcgccagg 2881 aaccccaccc aggaccgcat cctggagatg aaggtgatgg agttcttcat gaaggtgtac 2941 ggctacaggg gagagcacct gggcggaagc agaaagcctg acggcgccat ctatacagtg 3001 ggcagcccca tcgattacgg cgtgatcgtg gacacaaagg cctacagcgg cggctacaat 3061 ctgcctatcg gccaggccga cgcgatgcag agctacgtgg aggagaacca gacccggaat 3121 aagcacatca accccaacga gtggtggaag gtgtacccta gcagcgtgac cgagttcaag 3181 ttcctgttcg tgagcggcca cttcaagggc aactacaagg cccagctgac caggctgaac 3241 cacatcacca actgcaatgg cgccgtgctg agcgtggagg agctgctgat cggcggcgag 3301 atgatcaaag ccggcaccct gacactggag gaggtgcggc gcaagttcaa caacggcgag 3361 atcaacttca gatcttgata ctagaggaga atttgtactt ccagtcatac tagagagcgc 3421 gtggagccat ccgcagtttg aaaaaggcgg tgcgagcggt ggcggcagcg gtggcagcgc 3481 gtggagccat ccgcagtttg aaaaatacta gaggaagact acaagggtgg tgcggagtac 3541 tgcttaagct atgaaacgga aatattgaca gtagaatatg gattattacc gattggtaaa 3601 attgtagaaa agcgcatcga atgtactgtt tatagcgttg ataataatgg aaatatttat 3661 acacaacctg tagcacaatg gcacgatcgc ggagaacaag aggtgtttga gtattgtttg 3721 gaagatggtt cattgattcg ggcaacaaaa gaccataagt ttatgactgt tgatggtcaa 3781 atgttgccaa ttgatgaaat atttgaacgt gaattggatt tgatgcgggt tgataatttg 3841 ccgaattaa //