V. Hax3-2x
type: ComponentDefinition
persistent identity: BBa K2075005
display id: BBa_K2075005
version: 1
was generated by: iGEM to SBOL conversion
description: Vector iGem_02_Hax3 2xNG
created: 2016-10-12T11:00:00Z
modified: 2016-10-29T12:35:57Z
mutable provenance: caggtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccatgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcaagtatcaggacatgatcgcagcgttgccagaggcgacacacgaagcgatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggtggcgggagagttgagaggtccaccgttacagttggacacaggccaacttctcaagattgcaaagcgtggcggcgtgaccgcagtggaggcagtgcatgcatggcgcaatgcactgacgggtgcccccctgaaccttaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctcaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgactccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcgcatggccttaccccggagcaggtggtggccatcgccagcAATGGCggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcAATGGCggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccggagcaggtggtggccatcgccagcaatagcggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctcaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgactccggagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatggcggcggcaagcaggcgcagcattgttgcccagttatctcgccctgatccgtcgttggccgcgttaaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgctggatgcagtgaaaaagggattgccgcacgcgccggccttgatcaaaagaaccaatcgccgtattcccgaacgcacatcccatcgcgttgcc
owned by: james
owned by: myers
mutable description: This part synthesize a transcription activator like (TAL) effector protein. They were found in Xanthomonas bacteria. These bacteria are plant pathogenes and infects plants via a secretion typ III system. In the plant cells these proteins binds promoter sites. This leads to a transcriptional activation of the genes behind the promoter. The proteins contains tandem repeats up to 34.5. Each repeat binds one nucleotide of the target sequenz. The 12. and 13. Aminoacid of each repeat leads to the specific binding of the DNA sequence. We circularised the TAL-effectors with the help of Inteins at the N- and C- Terminus of each TAL-effector. The 12. And 13. Aminoacid of each of the 11.5 repeats from our TAL Hax3 ??? 2xNG (B) are: NI HD NI HD HD HD NG NG NS HD NI NG. They bind the DNA sequence: A C A C C C T T N C A T
discontinued: false
dominant: false
experience: experience/Works
group u list: _2543_
m user id: 33470
owner id: 33470
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: No considerations
star: false
creator: Kim Luehmann
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2075000
component: BBa_K2075003
component: BBa_K2075023
component: BBa_K2075001
component: BBa_K2075024
component: BBa_K2075010
sequence annotation: BBa_K2075010
sequence annotation: BBa_K2075001
sequence annotation: BBa_K2075023
sequence annotation: BBa_K2075003
sequence annotation: BBa_K2075000
sequence annotation: BBa_K2075024
sequence: https://synbiohub.org/public/igem/BBa_K2075005_sequence/1

BBa_K2075000
type: Component
persistent identity: component2530955
display id: component2530955
version: 1
top level: V. Hax3-2x
definition: Strep Tag
access: public

BBa_K2075003
type: Component
persistent identity: component2530953
display id: component2530953
version: 1
top level: V. Hax3-2x
definition: TEV Site
access: public

BBa_K2075023
type: Component
persistent identity: component2530951
display id: component2530951
version: 1
top level: V. Hax3-2x
definition: Hax3-2xNG
access: public

BBa_K2075001
type: Component
persistent identity: component2530946
display id: component2530946
version: 1
top level: V. Hax3-2x
definition: eGFP
access: public

BBa_K2075024
type: Component
persistent identity: component2530958
display id: component2530958
version: 1
top level: V. Hax3-2x
definition: Intein C-t
access: public

BBa_K2075010
type: Component
persistent identity: component2530944
display id: component2530944
version: 1
top level: V. Hax3-2x
definition: Intein N-t
access: public

BBa_K2075010
type: SequenceAnnotation
persistent identity: annotation2530944
display id: annotation2530944
version: 1
top level: V. Hax3-2x
location: https://synbiohub.org/public/igem/BBa_K2075005/annotation2530944/range2530944/1
component: BBa_K2075010

https://synbiohub.org/public/igem/BBa_K2075005/annotation2530944/range2530944/1
type: Range
persistent identity: range2530944
display id: range2530944
version: 1
top level: V. Hax3-2x
direction: direction/reverse
start: 1
end: 162
orientation: inline

BBa_K2075001
type: SequenceAnnotation
persistent identity: annotation2530946
display id: annotation2530946
version: 1
top level: V. Hax3-2x
location: https://synbiohub.org/public/igem/BBa_K2075005/annotation2530946/range2530946/1
component: BBa_K2075001

https://synbiohub.org/public/igem/BBa_K2075005/annotation2530946/range2530946/1
type: Range
persistent identity: range2530946
display id: range2530946
version: 1
top level: V. Hax3-2x
direction: direction/reverse
start: 169
end: 885
orientation: inline

BBa_K2075023
type: SequenceAnnotation
persistent identity: annotation2530951
display id: annotation2530951
version: 1
top level: V. Hax3-2x
location: https://synbiohub.org/public/igem/BBa_K2075005/annotation2530951/range2530951/1
component: BBa_K2075023

https://synbiohub.org/public/igem/BBa_K2075005/annotation2530951/range2530951/1
type: Range
persistent identity: range2530951
display id: range2530951
version: 1
top level: V. Hax3-2x
direction: direction/reverse
start: 894
end: 2696
orientation: inline

BBa_K2075003
type: SequenceAnnotation
persistent identity: annotation2530953
display id: annotation2530953
version: 1
top level: V. Hax3-2x
location: https://synbiohub.org/public/igem/BBa_K2075005/annotation2530953/range2530953/1
component: BBa_K2075003

https://synbiohub.org/public/igem/BBa_K2075005/annotation2530953/range2530953/1
type: Range
persistent identity: range2530953
display id: range2530953
version: 1
top level: V. Hax3-2x
direction: direction/reverse
start: 2705
end: 2725
orientation: inline

BBa_K2075000
type: SequenceAnnotation
persistent identity: annotation2530955
display id: annotation2530955
version: 1
top level: V. Hax3-2x
location: https://synbiohub.org/public/igem/BBa_K2075005/annotation2530955/range2530955/1
component: BBa_K2075000

https://synbiohub.org/public/igem/BBa_K2075005/annotation2530955/range2530955/1
type: Range
persistent identity: range2530955
display id: range2530955
version: 1
top level: V. Hax3-2x
direction: direction/reverse
start: 2734
end: 2823
orientation: inline

BBa_K2075024
type: SequenceAnnotation
persistent identity: annotation2530958
display id: annotation2530958
version: 1
top level: V. Hax3-2x
location: https://synbiohub.org/public/igem/BBa_K2075005/annotation2530958/range2530958/1
component: BBa_K2075024

https://synbiohub.org/public/igem/BBa_K2075005/annotation2530958/range2530958/1
type: Range
persistent identity: range2530958
display id: range2530958
version: 1
top level: V. Hax3-2x
direction: direction/reverse
start: 2832
end: 3167
orientation: inline

Strep Tag
type: ComponentDefinition
persistent identity: BBa K2075000
display id: BBa_K2075000
version: 1
was generated by: iGEM to SBOL conversion
description: Strep Tag
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T11:01:12Z
mutable provenance: Strep XT: agcgcgtggagccatccgcagtttgaaaaaggcggtgcgagcggtggcggcagcggtggcagcgcgtggagccatccgcagtttgaaaaa Strep II: tggagccatccgcagtttgaaaaa
owned by: james
owned by: myers
mutable description: To verify our circulate TAL-effector we added the Strep Tag with the two linker sequences by gBlocks. We used the Strep Tag for the purification of the vector and for detection. We have decided to take a Strep Tag because this Tag does not have to be on the end of a protein so it works even if the protein is circulate. This is different to the other Tag Sequences e.g. His Tag. In one vector (iGEM02_Ax7R-RR) we used the Strep-Tag II which binds Strep-Tactin an engineered form of streptavidin. Here are two Sequences of this Tag. Everyone have a size of 24 bp. In the three others vectors(iGEM02_GFP-Hax3_2xNN (C), iGEM02_GFP-Hax3_2xNG (B), iGEM02_Ax7L-DS) we used the Strep-Tactin(R)XT enables new applications in the field of high throughput screening, batch purification, purification using denaturing conditions and protein interaction studies. It has a size of 90 bp.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2543_
m user id: 33470
owner id: 33470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No considerations.
star: false
creator: Kim Luehmann
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: Strep TX Tag
sequence: https://synbiohub.org/public/igem/BBa_K2075000_sequence/1

Strep TX Tag
type: SequenceAnnotation
persistent identity: annotation2499084
display id: annotation2499084
version: 1
top level: Strep Tag
location: https://synbiohub.org/public/igem/BBa_K2075000/annotation2499084/range2499084/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K2075000/annotation2499084/range2499084/1
type: Range
persistent identity: range2499084
display id: range2499084
version: 1
top level: Strep Tag
direction: direction/forward
start: 1
end: 90
orientation: inline

eGFP
type: ComponentDefinition
persistent identity: BBa K2075001
display id: BBa_K2075001
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced GFP
created: 2016-10-12T11:00:00Z
modified: 2016-10-18T02:03:23Z
mutable provenance: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
owned by: james
owned by: myers
mutable description: The eGFP is a part which synthesize a green fluorescence protein (GFP). Exposed under blue or UV light it emits a bright green light. The enhanced GFP (eGFP) has a higher intensity emission than the wildtyp GFP. The origin of the GFP is the jellyfish Aequorea victoria. Today the GFP is an essential fluorescence reporter protein for biologie especially cellbiologie. It can be connect to other proteins to detect the expression of these proteins and their location. We used the eGFP to detect our TAL effectors on a chip, spotted with oligonucleotides. these oligonucleotides includes the target sequenz of the TALE Hax3 ??? 2xNN (A C A C C C G/A G/A ? C A).
discontinued: false
dominant: false
experience: experience/Works
group u list: _2543_
m user id: 33470
owner id: 33470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No considerations.
star: false
creator: Kim Luehmann
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: egfp
sequence: https://synbiohub.org/public/igem/BBa_K2075001_sequence/1

egfp
type: SequenceAnnotation
persistent identity: annotation2499119
display id: annotation2499119
version: 1
top level: eGFP
location: https://synbiohub.org/public/igem/BBa_K2075001/annotation2499119/range2499119/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K2075001/annotation2499119/range2499119/1
type: Range
persistent identity: range2499119
display id: range2499119
version: 1
top level: eGFP
direction: direction/forward
start: 1
end: 717
orientation: inline

TEV Site
type: ComponentDefinition
persistent identity: BBa K2075003
display id: BBa_K2075003
version: 1
was generated by: iGEM to SBOL conversion
description: TEV cleavage site
created: 2016-10-12T11:00:00Z
modified: 2016-10-18T02:03:53Z
mutable provenance: gagaatttgtacttccagtca
owned by: james
owned by: myers
mutable description: The TEV site is a DNA Sequenz encoding an aminoacid sequenz, which is cut by the TEV protease. The TEV protease is an protease with a high sequence specifity. The origin of the TEV protease is the tobacco each virus. We use the TEV Site to get our circularised TALE protein back into a linear form. Only the linear TAL effector protein can bind the target sequenz.
discontinued: false
dominant: false
experience: experience/None
group u list: _2543_
m user id: 33470
owner id: 33470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No considerations
star: false
creator: Kim Luehmann
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TEV Site
sequence: https://synbiohub.org/public/igem/BBa_K2075003_sequence/1

TEV Site
type: SequenceAnnotation
persistent identity: annotation2499185
display id: annotation2499185
version: 1
top level: TEV Site
location: https://synbiohub.org/public/igem/BBa_K2075003/annotation2499185/range2499185/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K2075003/annotation2499185/range2499185/1
type: Range
persistent identity: range2499185
display id: range2499185
version: 1
top level: TEV Site
direction: direction/forward
start: 1
end: 21
orientation: inline

Intein N-t
type: ComponentDefinition
persistent identity: BBa K2075010
display id: BBa_K2075010
version: 1
was generated by: iGEM to SBOL conversion
description: Intein and Intein linker (N-term)
created: 2016-10-13T11:00:00Z
modified: 2016-10-29T12:33:31Z
mutable provenance: taggcgtatcacgaggcagaatttcagatgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgtttcaatggcggttcgggcggttcgggcggcacaggcggttcgggcggttcgaagactacaagggtggtgcggagtactgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaa
owned by: james
owned by: myers
mutable description: Inteins were integrated into proteins. They useful by the circularization of a protein by ligating the N- and C-terminal ends. The team Heidelberg 2014 already used them for their circulation but we changed the linker sequence to make the circularization more accurate. They successful showed that circularization enhances resistance against proteases and denaturation. We show that circularization with a TAL-effector makes them more solid and durable for everyday laboratory. If you want more Information have a look of our results. The intein sequence have a size of 309 bp.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2543_
m user id: 33470
owner id: 33470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No considerations
star: false
creator: Kim Luehmann
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Npu DnaE(C) peptide
sequence annotation: Linker N-Term
sequence: https://synbiohub.org/public/igem/BBa_K2075010_sequence/1

Npu DnaE(C) peptide
type: SequenceAnnotation
persistent identity: annotation2525928
display id: annotation2525928
version: 1
top level: Intein N-t
location: https://synbiohub.org/public/igem/BBa_K2075010/annotation2525928/range2525928/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2075010/annotation2525928/range2525928/1
type: Range
persistent identity: range2525928
display id: range2525928
version: 1
top level: Intein N-t
direction: direction/forward
start: 1
end: 117
orientation: inline

Linker N-Term
type: SequenceAnnotation
persistent identity: annotation2530893
display id: annotation2530893
version: 1
top level: Intein N-t
location: https://synbiohub.org/public/igem/BBa_K2075010/annotation2530893/range2530893/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K2075010/annotation2530893/range2530893/1
type: Range
persistent identity: range2530893
display id: range2530893
version: 1
top level: Intein N-t
direction: direction/forward
start: 118
end: 162
orientation: inline

Intein C-t
type: ComponentDefinition
persistent identity: BBa K2075024
display id: BBa_K2075024
version: 1
was generated by: iGEM to SBOL conversion
description: Intein and Intein Linker (C-term)
created: 2016-10-17T11:00:00Z
modified: 2016-10-18T02:05:16Z
mutable provenance: Heidelberg 2014
owned by: james
owned by: myers
mutable description: Inteins were integrated into proteins. They useful by the circularization of a protein by ligating the N- and C-terminal ends. The team Heidelberg 2014 already used them for their circulation but we changed the linker sequence to make the circularization more accurate. They successful showed that circularization enhances resistance against proteases and denaturation. We show that circularization with a TAL-effector makes them more solid and durable for everyday laboratory. If you want more Information have a look of our results. The intein sequence have a size of 309 bp.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2543_
m user id: 33470
owner id: 33470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We wanted to improve the circularization of our TAL effector
star: false
creator: Kim Luehmann
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Intein linker (C-term)
sequence annotation: Intein (C-term)
sequence: https://synbiohub.org/public/igem/BBa_K2075024_sequence/1

Intein linker (C-term)
type: SequenceAnnotation
persistent identity: annotation2516444
display id: annotation2516444
version: 1
top level: Intein C-t
location: https://synbiohub.org/public/igem/BBa_K2075024/annotation2516444/range2516444/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2075024/annotation2516444/range2516444/1
type: Range
persistent identity: range2516444
display id: range2516444
version: 1
top level: Intein C-t
direction: direction/forward
start: 1
end: 27
orientation: inline

Intein (C-term)
type: SequenceAnnotation
persistent identity: annotation2516445
display id: annotation2516445
version: 1
top level: Intein C-t
location: https://synbiohub.org/public/igem/BBa_K2075024/annotation2516445/range2516445/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2075024/annotation2516445/range2516445/1
type: Range
persistent identity: range2516445
display id: range2516445
version: 1
top level: Intein C-t
direction: direction/forward
start: 28
end: 336
orientation: inline

Hax3-2xNG
type: ComponentDefinition
persistent identity: BBa K2075023
display id: BBa_K2075023
version: 1
was generated by: iGEM to SBOL conversion
description: TAL-effector Hax3-2xNG
created: 2016-10-13T11:00:00Z
modified: 2016-10-18T01:51:50Z
mutable provenance: Xanthomonas
owned by: james
owned by: myers
mutable description: Designing cyclic TALEs allows a regulation of those proteins, because of topological problems. A TALE is always winding itself around the DNA to bind. If the protein is cyclic, this is no longer possible and the TALE-bond is inhibited. This could also be used for appliances concerning drug delivery. If the cyclic bond is irreversible and a protease can cut the protein, the TALE regains full transcriptional activity (Lonzarić, 2016). To prove this statement, we inserted a TEV cleavage site from the tobacco each virus into the vector which enables induced linearization of the protein after expression with ProTEV Plus protease (Promega). This composite part includes an Intein and linker, an eGFP, a TAL-effector, a TEV Site and a Strep Tag. They translated all together. The intein and linker are important to circularice the aminoacid sequence between the N- and C-terminal parts. Between the Intein sites is an eGFP and the TAL-effector in frame. There is also a strep XT tag and the TEV Site. When it is translated the intein sites react and form a circularised protein. All the parts between N- and C-Terminal Intein is part of the protein circle. In this circle the hole protein gains more stability. With the help of the strep XT tag the protein can be purified even if it is circularised. The Strep XT Tag is also usefull to detect the protein with an immunostain. The TEV Site give the opportunity to linearise the protein again wit the help oft he TEV protease. The linearised TAL can bind the specific DNA Sequence. The eGFP gives us the opportunity to detect the connected TAL-effector. The TAL effector is the Hax3-2xNG. The 12th and 13th amino acid of each of the 11.5 repeats from our TAL Hax3 ??? 2xNG (B) are: NI HD NI HD HD HD NG NG NS HD NI NG. They bind the DNA sequence: A C A C C C T T N C A T
discontinued: false
dominant: false
experience: experience/Works
group u list: _2543_
m user id: 33470
owner id: 33470
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: This composite part includes an Intein and linker, an eGFP, a TAL-effector, a TEV Site and a Strep Tag. They translated all together. The intein and linker are important to circularice the aminoacid sequence between the N- and C-terminal parts. Between the Intein sites is an eGFP and the TAL-effector in frame. There is also a strep XT tag and the TEV Site. When it is translated the intein sites react and form a circularised protein. All the parts between N- and C-Terminal Intein is part of the protein circle. In this circle the hole protein gains more stability. With the help of the strep XT tag the protein can be purified even if it is circularised. The Strep XT Tag is also usefull to detect the protein with an immunostain. The TEV Site give the opportunity to linearise the protein again wit the help oft he TEV protease. The linearised TAL can bind the specific DNA Sequence. The eGFP gives us the opportunity to detect the connected TAL-effector. The TAL effector is the Hax3-2xNG. The 12th and 13th amino acid of each of the 11.5 repeats from our TAL Hax3 ??? 2xNG (B) are: NI HD NI HD HD HD NG NG NS HD NI NG. They bind the DNA sequence: A C A C C C T T N C A T
star: false
creator: Kim Luehmann
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: NLS SV40
sequence annotation: N-Term Hax3 137
sequence annotation: Hax3
sequence annotation: C-Term Hax3 63
sequence: https://synbiohub.org/public/igem/BBa_K2075023_sequence/1

NLS SV40
type: SequenceAnnotation
persistent identity: annotation2518324
display id: annotation2518324
version: 1
top level: Hax3-2xNG
location: https://synbiohub.org/public/igem/BBa_K2075023/annotation2518324/range2518324/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2075023/annotation2518324/range2518324/1
type: Range
persistent identity: range2518324
display id: range2518324
version: 1
top level: Hax3-2xNG
direction: direction/forward
start: 1
end: 21
orientation: inline

N-Term Hax3 137
type: SequenceAnnotation
persistent identity: annotation2518325
display id: annotation2518325
version: 1
top level: Hax3-2xNG
location: https://synbiohub.org/public/igem/BBa_K2075023/annotation2518325/range2518325/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2075023/annotation2518325/range2518325/1
type: Range
persistent identity: range2518325
display id: range2518325
version: 1
top level: Hax3-2xNG
direction: direction/forward
start: 22
end: 433
orientation: inline

Hax3
type: SequenceAnnotation
persistent identity: annotation2518327
display id: annotation2518327
version: 1
top level: Hax3-2xNG
location: https://synbiohub.org/public/igem/BBa_K2075023/annotation2518327/range2518327/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2075023/annotation2518327/range2518327/1
type: Range
persistent identity: range2518327
display id: range2518327
version: 1
top level: Hax3-2xNG
direction: direction/forward
start: 434
end: 1611
orientation: inline

C-Term Hax3 63
type: SequenceAnnotation
persistent identity: annotation2518358
display id: annotation2518358
version: 1
top level: Hax3-2xNG
location: https://synbiohub.org/public/igem/BBa_K2075023/annotation2518358/range2518358/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2075023/annotation2518358/range2518358/1
type: Range
persistent identity: range2518358
display id: range2518358
version: 1
top level: Hax3-2xNG
direction: direction/forward
start: 1612
end: 1803
orientation: inline

https://synbiohub.org/public/igem/BBa_K2075005_sequence/1
type: Sequence
persistent identity: BBa K2075005 sequence
display id: BBa_K2075005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgtttcaatggcggttcgggcggttcgggcggcacaggcggttcgggcggttcttactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtactagaggccaagaagaagaggaaggtgcaggtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccatgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcaagtatcaggacatgatcgcagcgttgccagaggcgacacacgaagcgatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggtggcgggagagttgagaggtccaccgttacagttggacacaggccaacttctcaagattgcaaagcgtggcggcgtgaccgcagtggaggcagtgcatgcatggcgcaatgcactgacgggtgcccccctgaaccttaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctcaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgactccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcgcatggccttaccccggagcaggtggtggccatcgccagcaatggcggtggcaagcaggcgctggagacg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2075000_sequence/1
type: Sequence
persistent identity: BBa K2075000 sequence
display id: BBa_K2075000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgcgtggagccatccgcagtttgaaaaaggcggtgcgagcggtggcggcagcggtggcagcgcgtggagccatccgcagtttgaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2075001_sequence/1
type: Sequence
persistent identity: BBa K2075001 sequence
display id: BBa_K2075001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2075003_sequence/1
type: Sequence
persistent identity: BBa K2075003 sequence
display id: BBa_K2075003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagaatttgtacttccagtca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2075010_sequence/1
type: Sequence
persistent identity: BBa K2075010 sequence
display id: BBa_K2075010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgtttcaatggcggttcgggcggttcgggcggcacaggcggttcgggcggttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2075024_sequence/1
type: Sequence
persistent identity: BBa K2075024 sequence
display id: BBa_K2075024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagactacaagggtggtgcggagtactgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2075023_sequence/1
type: Sequence
persistent identity: BBa K2075023 sequence
display id: BBa_K2075023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccaagaagaagaggaaggtgcaggtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccatgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcaagtatcaggacatgatcgcagcgttgccagaggcgacacacgaagcgatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggtggcgggagagttgagaggtccaccgttacagttggacacaggccaacttctcaagattgcaaagcgtggcggcgtgaccgcagtggaggcagtgcatgcatggcgcaatgcactgacgggtgcccccctgaaccttaccccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccggagcaggtggtggccatcgccagcaatattggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctcaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgactccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcgcatggccttaccccggagcaggtggtggccatcgccagcaatggcggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatggcggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgacaccggagcaggtggtggccatcgccagcaatagcggtggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctcaccccggagcaggtggtggccatcgccagccacgatggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgactccggagcaggtggtggccatcgccagcaatattggcggcaagcaggcgctggagacggtgcagcggctgttgccggtgctgtgccaggcccatggcctgaccccggagcaggtggtggccatcgccagcaatggcggcggcaagcaggcgctggagagcattgttgcccagttatctcgccctgatccgtcgttggccgcgttaaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgctggatgcagtgaaaaagggattgccgcacgcgccggccttgatcaaaagaaccaatcgccgtattcccgaacgcacatcccatcgcgttgcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z