LOCUS BBa_K2075007 3587 bp DNA linear 22-Nov-2024 DEFINITION Vector iGem_02 Ax7R- RR ACCESSION BBa_K2075007 VERSION BBa_K2075007.1 FEATURES Location/Qualifiers CDS 1..117 /label=Npu DnaE(C) peptide CDS 118..162 /label=Linker N-Term CDS 1..162 /label=BBa_K2075010 CDS 171..3116 /label=Ax7R-RR CDS 171..3116 /label=BBa_K2075021 misc_feature 3125..3145 /label=TEV Site misc_feature 3125..3145 /label=BBa_K2075003 misc_feature 3154..3243 /label=Strep TX Tag misc_feature 3154..3243 /label=BBa_K2075000 CDS 3252..3278 /label=Intein linker (C-term) CDS 3279..3587 /label=Intein (C-term) CDS 3252..3587 /label=BBa_K2075024 ORIGIN 1 atgatcaaaa tagccacacg taaatattta ggcaaacaaa atgtctatga cattggagtt 61 gagcgcgacc ataattttgc actcaaaaat ggcttcatag cttccaactg tttcaatggc 121 ggttcgggcg gttcgggcgg cacaggcggt tcgggcggtt cttactagag tatgtaccca 181 tacgatgttc ctgactatgc ggccaagaag aagaggaagg tgcaggtgga tctacgcacg 241 ctcggctaca gccagcagca acaggagaag atcaaaccga aggttcgttc gacagtggcg 301 cagcaccacg aggcactggt cggccatggg tttacacacg cgcacatcgt tgcgctcagc 361 caacacccgg cagcgttagg gaccgtcgct gtcaagtatc aggacatgat cgcagcgttg 421 ccagaggcga cacacgaagc gatcgttggc gtcggcaaac agtggtccgg cgcacgcgct 481 ctggaggcct tgctcacggt ggcgggagag ttgagaggtc caccgttaca gttggacaca 541 ggccaacttc tcaagattgc aaagcgtggc ggcgtgaccg cagtggaggc agtgcatgca 601 tggcgcaatg cactgacggg tgcccccctg aaccttaccc cggagcaggt ggtggccatc 661 gccagcaatg gcggtggcaa gcaggcgctg gagacggtgc agcggctgtt gccggtgctg 721 tgccaggccc atggcctgac cccggagcag gtggtggcca tcgccagcaa taacggcggc 781 aagcaggcgc tggagacggt gcagcggctg ttgccggtgc tgtgccaggc ccatggcctg 841 acaccggagc aggtggtggc catcgccagc aatattggtg gcaagcaggc gctggagacg 901 gtgcagcggc tgttgccggt gctgtgccag gcccatggcc tcaccccgga gcaggtggtg 961 gccatcgcca gcaatattgg cggcaagcag gcgctggaga cggtgcagcg gctgttgccg 1021 gtgctgtgcc aggcccatgg cctgactccg gagcaggtgg tggccatcgc cagcaataac 1081 ggcggcaagc aggcgctgga gacggtgcag cggctgttgc cggtgctgtg ccaggcccat 1141 ggcctgaccc cggagcaggt ggtggccatc gccagcaata acggcggcaa gcaggcgctg 1201 gagacggtgc agcggctgtt gccggtgctg tgccaggcgc atggccttac cccggagcag 1261 gtggtggcca tcgccagcca cgatggtggc aagcaggcgc tggagacggt gcagcggctg 1321 ttgccggtgc tgtgccaggc ccatggcctg accccggagc aggtggtggc catcgccagc 1381 aatggcggcg gcaagcaggc gctggagacg gtgcagcggc tgttgccggt gctgtgccag 1441 gcccatggcc tgacaccgga gcaggtggtg gccatcgcca gcaatggcgg tggcaagcag 1501 gcgctggaga cggtgcagcg gctgttgccg gtgctgtgcc aggcccatgg cctcaccccg 1561 gagcaggtgg tggccatcgc cagcaataac ggcggcaagc aggcgctgga gacggtgcag 1621 cggctgttgc cggtgctgtg ccaggcccat ggcctgactc cggagcaggt ggtggccatc 1681 gccagcaata ttggcggcaa gcaggcgctg gagacggtgc agcggctgtt gccggtgctg 1741 tgccaggccc atggcctgac cccggagcag gtggtggcca tcgccagcaa tggcggcggc 1801 aagcaggcgc tggagacggt gcagcggctg ttgccggtgc tgtgccaggc gcatggcctt 1861 accccggagc aggtggtggc catcgccagc aataacggtg gcaagcaggc gctggagacg 1921 gtgcagcggc tgttgccggt gctgtgccag gcccatggcc tgaccccgga gcaggtggtg 1981 gccatcgcca gcaatattgg cggcaagcag gcgctggaga cggtgcagcg gctgttgccg 2041 gtgctgtgcc aggcccatgg cctgacaccg gagcaggtgg tggccatcgc cagcaataac 2101 ggtggcaagc aggcgctgga gacggtgcag cggctgttgc cggtgctgtg ccaggcccat 2161 ggcctcaccc cggagcaggt ggtggccatc gccagccacg atggcggcaa gcaggcgctg 2221 gagacggtgc agcggctgtt gccggtgctg tgccaggccc atggcctgac tccggagcag 2281 gtggtggcca tcgccagcaa tggcggcggc aagcaggcgc tggagagcat tgttgcccag 2341 ttatctcgcc ctgatccgtc gttggccgcg ttaaccaacg accacctcgt cgccttggcc 2401 tgcctcggcg gacgtcctgc gctggatgca gtgaaaaagg gattgccgca cgcgccggcc 2461 ttgatcaaaa gaaccaatcg ccgtattccc gaacgcacat cccatcgcgt tgccggatcc 2521 cagctggtga agagcgagct ggaggagaag aagtccgagc tgcggcacaa gctgaagtac 2581 gtgccccacg agtacatcga gctgatcgag atcgccagga accccaccca ggaccgcatc 2641 ctggagatga aggtgatgga gttcttcatg aaggtgtacg gctacagggg agagcacctg 2701 ggcggaagca gaaagcctga cggcgccatc tatacagtgg gcagccccat cgattacggc 2761 gtgatcgtgg acacaaaggc ctacagcggc ggctacaatc tgcctatcgg ccaggccaga 2821 gagatgcaga gatacgtgga ggagaaccag acccggaata agcacatcaa ccccaacgag 2881 tggtggaagg tgtaccctag cagcgtgacc gagttcaagt tcctgttcgt gagcggccac 2941 ttcaagggca actacaaggc ccagctgacc aggctgaacc acatcaccaa ctgcaatggc 3001 gccgtgctga gcgtggagga gctgctgatc ggcggcgaga tgatcaaagc cggcaccctg 3061 acactggagg aggtgcggcg caagttcaac aacggcgaga tcaacttcag atcttgtact 3121 agaggagaat ttgtacttcc agtcatacta gagagcgcgt ggagccatcc gcagtttgaa 3181 aaaggcggtg cgagcggtgg cggcagcggt ggcagcgcgt ggagccatcc gcagtttgaa 3241 aaatactaga ggaagactac aagggtggtg cggagtactg cttaagctat gaaacggaaa 3301 tattgacagt agaatatgga ttattaccga ttggtaaaat tgtagaaaag cgcatcgaat 3361 gtactgttta tagcgttgat aataatggaa atatttatac acaacctgta gcacaatggc 3421 acgatcgcgg agaacaagag gtgtttgagt attgtttgga agatggttca ttgattcggg 3481 caacaaaaga ccataagttt atgactgttg atggtcaaat gttgccaatt gatgaaatat 3541 ttgaacgtga attggatttg atgcgggttg ataatttgcc gaattaa //