LOCUS BBa_K2075022 3167 bp DNA linear 23-Dec-2024 DEFINITION Vector iGEM_02_Hax3_2xNN ACCESSION BBa_K2075022 VERSION BBa_K2075022.1 FEATURES Location/Qualifiers CDS 1..117 /label=Npu DnaE(C) peptide CDS 118..162 /label=Linker N-Term CDS 1..162 /label=BBa_K2075010 CDS 169..885 /label=egfp CDS 169..885 /label=BBa_K2075001 CDS 894..914 /label=SV40 NLS CDS 915..1327 /label=N-Term Hax3 CDS 1328..2505 /label=Hax3-2xNN CDS 2506..2696 /label=C-Term Hax3 63 CDS 894..2696 /label=BBa_K2075009 misc_feature 2705..2725 /label=TEV Site misc_feature 2705..2725 /label=BBa_K2075003 misc_feature 2734..2823 /label=Strep TX Tag misc_feature 2734..2823 /label=BBa_K2075000 CDS 2832..2858 /label=Intein linker (C-term) CDS 2859..3167 /label=Intein (C-term) CDS 2832..3167 /label=BBa_K2075024 ORIGIN 1 atgatcaaaa tagccacacg taaatattta ggcaaacaaa atgtctatga cattggagtt 61 gagcgcgacc ataattttgc actcaaaaat ggcttcatag cttccaactg tttcaatggc 121 ggttcgggcg gttcgggcgg cacaggcggt tcgggcggtt cttactagat ggtgagcaag 181 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 241 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 301 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 361 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 421 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 481 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 541 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 601 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 661 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 721 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 781 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 841 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtacta gaggccaaga 901 agaagaggaa ggtgcaggtg gatctacgca cgctcggcta cagccagcag caacaggaga 961 agatcaaacc gaaggttcgt tcgacagtgg cgcagcacca cgaggcactg gtcggccatg 1021 ggtttacaca cgcgcacatc gttgcgctca gccaacaccc ggcagcgtta gggaccgtcg 1081 ctgtcaagta tcaggacatg atcgcagcgt tgccagaggc gacacacgaa gcgatcgttg 1141 gcgtcggcaa acagtggtcc ggcgcacgcg ctctggaggc cttgctcacg gtggcgggag 1201 agttgagagg tccaccgtta cagttggaca caggccaact tctcaagatt gcaaagcgtg 1261 gcggcgtgac cgcagtggag gcagtgcatg catggcgcaa tgcactgacg ggtgcccccc 1321 tgaaccttac cccggagcag gtggtggcca tcgccagcaa tattggtggc aagcaggcgc 1381 tggagacggt gcagcggctg ttgccggtgc tgtgccaggc ccatggcctg accccggagc 1441 aggtggtggc catcgccagc cacgatggcg gcaagcaggc gctggagacg gtgcagcggc 1501 tgttgccggt gctgtgccag gcccatggcc tgacaccgga gcaggtggtg gccatcgcca 1561 gcaatattgg tggcaagcag gcgctggaga cggtgcagcg gctgttgccg gtgctgtgcc 1621 aggcccatgg cctcaccccg gagcaggtgg tggccatcgc cagccacgat ggcggcaagc 1681 aggcgctgga gacggtgcag cggctgttgc cggtgctgtg ccaggcccat ggcctgactc 1741 cggagcaggt ggtggccatc gccagccacg atggcggcaa gcaggcgctg gagacggtgc 1801 agcggctgtt gccggtgctg tgccaggccc atggcctgac cccggagcag gtggtggcca 1861 tcgccagcca cgatggcggc aagcaggcgc tggagacggt gcagcggctg ttgccggtgc 1921 tgtgccaggc gcatggcctt accccggagc aggtggtggc catcgccagc aataacggtg 1981 gcaagcaggc gctggagacg gtgcagcggc tgttgccggt gctgtgccag gcccatggcc 2041 tgaccccgga gcaggtggtg gccatcgcca gcaataacgg cggcaagcag gcgctggaga 2101 cggtgcagcg gctgttgccg gtgctgtgcc aggcccatgg cctgacaccg gagcaggtgg 2161 tggccatcgc cagcaatagc ggtggcaagc aggcgctgga gacggtgcag cggctgttgc 2221 cggtgctgtg ccaggcccat ggcctcaccc cggagcaggt ggtggccatc gccagccacg 2281 atggcggcaa gcaggcgctg gagacggtgc agcggctgtt gccggtgctg tgccaggccc 2341 atggcctgac tccggagcag gtggtggcca tcgccagcaa tattggcggc aagcaggcgc 2401 tggagacggt gcagcggctg ttgccggtgc tgtgccaggc ccatggcctg accccggagc 2461 aggtggtggc catcgccagc aatggcggcg gcaagcaggc gctggagagc attgttgccc 2521 agttatctcg ccctgatccg tcgttggccg cgttaaccaa cgaccacctc gtcgccttgg 2581 cctgcctcgg cggacgtcct gcgctggatg cagtgaaaaa gggattgccg cacgcgccgg 2641 ccttgatcaa aagaaccaat cgccgtattc ccgaacgcac atcccatcgc gttgcctact 2701 agaggagaat ttgtacttcc agtcatacta gagagcgcgt ggagccatcc gcagtttgaa 2761 aaaggcggtg cgagcggtgg cggcagcggt ggcagcgcgt ggagccatcc gcagtttgaa 2821 aaatactaga ggaagactac aagggtggtg cggagtactg cttaagctat gaaacggaaa 2881 tattgacagt agaatatgga ttattaccga ttggtaaaat tgtagaaaag cgcatcgaat 2941 gtactgttta tagcgttgat aataatggaa atatttatac acaacctgta gcacaatggc 3001 acgatcgcgg agaacaagag gtgtttgagt attgtttgga agatggttca ttgattcggg 3061 caacaaaaga ccataagttt atgactgttg atggtcaaat gttgccaatt gatgaaatat 3121 ttgaacgtga attggatttg atgcgggttg ataatttgcc gaattaa //