BBa_K2075024 1 Intein C-t Intein and Intein Linker (C-term) 2016-10-17T11:00:00Z 2016-10-18T02:05:16Z Heidelberg 2014 Inteins were integrated into proteins. They useful by the circularization of a protein by ligating the N- and C-terminal ends. The team Heidelberg 2014 already used them for their circulation but we changed the linker sequence to make the circularization more accurate. They successful showed that circularization enhances resistance against proteases and denaturation. We show that circularization with a TAL-effector makes them more solid and durable for everyday laboratory. If you want more Information have a look of our results. The intein sequence have a size of 309 bp. false false _2543_ 33470 33470 9 false We wanted to improve the circularization of our TAL effector false Kim Luehmann annotation2516445 1 Intein (C-term) range2516445 1 28 336 annotation2516444 1 Intein linker (C-term) range2516444 1 1 27 BBa_K2075010 1 Intein N-t Intein and Intein linker (N-term) 2016-10-13T11:00:00Z 2016-10-29T12:33:31Z taggcgtatcacgaggcagaatttcagatgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgtttcaatggcggttcgggcggttcgggcggcacaggcggttcgggcggttcgaagactacaagggtggtgcggagtactgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaa Inteins were integrated into proteins. They useful by the circularization of a protein by ligating the N- and C-terminal ends. The team Heidelberg 2014 already used them for their circulation but we changed the linker sequence to make the circularization more accurate. They successful showed that circularization enhances resistance against proteases and denaturation. We show that circularization with a TAL-effector makes them more solid and durable for everyday laboratory. If you want more Information have a look of our results. The intein sequence have a size of 309 bp. false false _2543_ 33470 33470 9 false No considerations false Kim Luehmann annotation2525928 1 Npu DnaE(C) peptide range2525928 1 1 117 annotation2530893 1 Linker N-Term range2530893 1 118 162 BBa_K2075025 1 Intein Intein N- and C-Term + Linker 2016-10-19T11:00:00Z 2016-10-29T12:38:13Z .. ... false false _2543_ 33470 31156 9 true .. false Theresa Schmidt component2530902 1 BBa_K2075010 component2530905 1 BBa_K2075024 annotation2530905 1 BBa_K2075024 range2530905 1 171 506 annotation2530902 1 BBa_K2075010 range2530902 1 1 162 BBa_K2075025_sequence 1 atgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgtttcaatggcggttcgggcggttcgggcggcacaggcggttcgggcggttcttactagaggaagactacaagggtggtgcggagtactgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaa BBa_K2075024_sequence 1 gaagactacaagggtggtgcggagtactgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaa BBa_K2075010_sequence 1 atgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgtttcaatggcggttcgggcggttcgggcggcacaggcggttcgggcggttct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z