LOCUS BBa_K2077000 5743 bp DNA linear 22-Nov-2024 DEFINITION pSB416-GPD: URA3-selectable S. cerevisiae Expression Vector (RFC 10) ACCESSION BBa_K2077000 VERSION BBa_K2077000.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=BB suffix misc_feature 23..270 /label=CYC1 Terminator CDS 1065..1868 /label=URA3 CDS misc_feature 478..933 /label=F1 ORI promoter 1869..2080 /label=URA3 Promoter misc_feature 2343..2864 /label=CEN6 Centromere promoter 2883..2987 /label=bla promoter CDS 2988..3848 /label=bla CDS misc_feature 4019..4607 /label=ColE1 ORI promoter 5073..5716 /label=GPD Promoter misc_feature 5722..5743 /label=BB prefix ORIGIN 1 tactagtagc ggccgctgca ggtcatgtaa ttagttatgt cacgcttaca ttcacgccct 61 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct 121 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt 181 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag 241 gttttgggac gctcgaaggc tttaatttgc ggccggtacc caattcgccc tatagtgagt 301 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg 481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 901 cgaattttaa caaaatatta acgtttacaa tttcctgatg cggtattttc tccttacgca 961 tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa 1021 tttgtgagtt tagtatacat gcatttactt ataatacagt ttttttagtt ttgctggccg 1081 catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca 1141 tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc 1201 acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca 1261 ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca 1321 ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat 1381 tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt 1441 tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca 1501 ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc 1561 aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac 1621 ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata 1681 tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat 1741 tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata 1801 ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct 1861 ttcgacatga tttatcttcg tttccggttt ttgttctgtg cagttgggtt aagaatactg 1921 ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct 1981 ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc 2041 gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aaggtggtat ggtgcactct 2101 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc 2161 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt 2221 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa 2281 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagga 2341 cggatcgctt gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg 2401 aatttactct gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa 2461 gaaggtagaa gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa 2521 caaaaagcgt actttacata tatatttatt agacaagaaa agcagattaa atagatatac 2581 attcgattaa cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac 2641 agacaagatg aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta 2701 gtatttgttg gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt 2761 ctttaatttc tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa 2821 ttataattat ttttatagca cgtgatgaaa aggacccagg tggcactttt cggggaaatg 2881 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 2941 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3001 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3061 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3121 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3181 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 3241 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3301 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3361 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3421 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3481 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 3541 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 3601 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 3661 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 3721 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 3781 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 3841 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 3901 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 3961 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4021 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4081 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4141 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 4201 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4261 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4321 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4381 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4441 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4501 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 4561 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 4621 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 4681 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 4741 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 4801 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 4861 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg 4921 caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat 4981 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc 5041 actaaaggga acaaaagctg gagctcagtt tatcattatc aatactcgcc atttcaaaga 5101 atacgtaaat aattaatagt agtgattttc ctaactttat ttagtcaaaa aattagcctt 5161 ttaattctgc tgtaacccgt acatgcccaa aatagggggc gggttacaca gaatatataa 5221 catcgtaggt gtctgggtga acagtttatt cctggcatcc actaaatata atggagcccg 5281 ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca 5341 ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac 5401 aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt aaatgatgac 5461 acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct 5521 gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt 5581 cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct 5641 atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac 5701 cagaacttag tttcgacgga agaattcgcg gccgcttcta gag //