LOCUS BBa_K2077001 5828 bp DNA linear 29-Sep-2024 DEFINITION pSB413-GPD: HIS3-selectable S. cerevisiae Expression Vector (RFC 10) ACCESSION BBa_K2077001 VERSION BBa_K2077001.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=BioBrick Suffix misc_feature 23..270 /label=CYC1 Terminator misc_feature 478..933 /label=F1 ORI CDS 1193..1852 /label=HIS3 promoter 1853..2039 /label=HIS3 Promoter misc_feature 2425..2928 /label=CEN 6 Centromere promoter 2965..3069 /label=bla promoter CDS 3070..3930 /label=Bla CDS misc_feature 4101..4689 /label=ColEI ORI promoter 5158..5801 /label=GPD Promoter misc_feature 5807..5828 /label=BioBrick Prefix ORIGIN 1 tactagtagc ggccgctgca ggtcatgtaa ttagttatgt cacgcttaca ttcacgccct 61 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct 121 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt 181 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag 241 gttttgggac gctcgaaggc tttaatttgc ggccggtacc caattcgccc tatagtgagt 301 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg 481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 901 cgaattttaa caaaatatta acgtttacaa tttcctgatg cggtattttc tccttacgca 961 tctgtgcggt atttcacacc gcatagatcc gtcgagttca agagaaaaaa aaagaaaaag 1021 caaaaagaaa aaaggaaagc gcgcctcgtt cagaatgaca cgtatagaat gatgcattac 1081 cttgtcatct tcagtatcat actgttcgta tacatactta ctgacattca taggtataca 1141 tatatacaca tgtatatata tcgtatgctg ctgctttaaa taatcggtgt cactacataa 1201 gaacaccttt ggtggaggga acatcgttgg taccattggg cgaggtggct tctcttatgg 1261 caaccgcaag agccttgaac gcactctcac tacggtgatg atcattcttg cctcgcagac 1321 aatcaacgtg gagggtaatt ctgctagcct ctgcaaagct ttcaagaaaa tgcgggatca 1381 tctcgcaaga gagatctcct actttctccc tttgcaaacc aagttcgaca actgcgtacg 1441 gcctgttcga aagatctacc accgctctgg aaagtgcctc atccaaaggc gcaaatcctg 1501 atccaaacct ttttactcca cgcgccagta gggcctcttt aaaagcttga ccgagagcaa 1561 tcccgcagtc ttcagtggtg tgatggtcgt ctatgtgtaa gtcaccaatg cactcaacga 1621 ttagcgacca gccggaatgc ttggccagag catgtatcat atggtccaga aaccctatac 1681 ctgtgtggac gttaatcact tgcgattgtg tggcctgttc tgctactgct tctgcctctt 1741 tttctgggaa gatcgagtgc tctatcgcta ggggaccacc ctttaaagag atcgcaatct 1801 gaatcttggt ttcatttgta atacgcttta ctagggcttt ctgctctgtc atctttgcct 1861 tcgtttatct tgcctgctca ttttttagta tattcttcga agaaatcaca ttactttata 1921 taatgtataa ttcattatgt gataatgcca atcgctaaga aaaaaaaaga gtcatccgct 1981 aggggaaaaa aaaaaatgaa aatcattacc gaggcataaa aaaatataga gtgtactaga 2041 ggaggccaag agtaatagaa aaagaaaatt gcgggaaagg actgtgttat gacttccctg 2101 actaatgccg tgttcaaacg atacctggca gtgactccta gcgctcacca agctcttaaa 2161 acgggaattt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 2221 cccgacaccc gccaacacgc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 2281 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 2341 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 2401 tgataataat ggtttcttag gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct 2461 tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt 2521 tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa 2581 acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga 2641 aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat 2701 tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc 2761 aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt 2821 cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata 2881 tatttatatt aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca 2941 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 3001 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 3061 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 3121 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 3181 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 3241 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 3301 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 3361 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 3421 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 3481 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 3541 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 3601 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 3661 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 3721 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 3781 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 3841 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 3901 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 3961 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 4021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 4081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 4141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 4201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 4261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 4321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 4381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 4441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 4501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 4561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 4621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 4681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 4741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 4801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 4861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 4921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 4981 gtgagttacc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg 5041 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 5101 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctctc gagtttatca 5161 ttatcaatac tcgccatttc aaagaatacg taaataatta atagtagtga ttttcctaac 5221 tttatttagt caaaaaatta gccttttaat tctgctgtaa cccgtacatg cccaaaatag 5281 ggggcgggtt acacagaata tataacatcg taggtgtctg ggtgaacagt ttattcctgg 5341 catccactaa atataatgga gcccgctttt taagctggca tccagaaaaa aaaagaatcc 5401 cagcaccaaa atattgtttt cttcaccaac catcagttca taggtccatt ctcttagcgc 5461 aactacagag aacaggggca caaacaggca aaaaacgggc acaacctcaa tggagtgatg 5521 caacctgcct ggagtaaatg atgacacaag gcaattgacc cacgcatgta tctatctcat 5581 tttcttacac cttctattac cttctgctct ctctgatttg gaaaaagctg aaaaaaaagg 5641 ttgaaaccag ttccctgaaa ttattcccct acttgactaa taagtatata aagacggtag 5701 gtattgattg taattctgta aatctatttc ttaaacttct taaattctac ttttatagtt 5761 agtctttttt ttagttttaa aacaccagaa cttagtttcg acggaagaat tcgcggccgc 5821 ttctagag //