BBa_K2077002 1 BBa_K2077002 mls-yeGFP 2016-08-16T11:00:00Z 2016-10-14T11:21:51Z TBA According to 2015 Rose-Hulman iGEM team, the coding sequence of mitochondrial ribosomal protein mRPS12 starts with a mitochondrial localization signal (mls). From previous research, it is possible that this mls protein is able to transport and direct any attached protein into the mitochondria. To verify the hypothesis brought up in 2015, we constructed a hybrid system with the mls protein and a reporter protein. Instead of using the regular GFP, yeast-enhanced green fluorescent protein (yeGFP) was used in this system as optimal gene expression can be achieved. false false _2545_ 31771 31901 9 false yeast codon optimized GFP is used instead of regular GFP. And a GS linker was used to create this mls-yeGFP hybrid system. false Xintong Xia, Xander Lacrampe, Richard Anthony annotation2481492 1 yeGFP range2481492 1 121 837 annotation2481489 1 RBS(WT mRPS12 Kozak) range2481489 1 1 22 annotation2481491 1 GS Linker range2481491 1 88 120 annotation2481490 1 mRPS12 mls range2481490 1 19 87 BBa_K2077002_sequence 1 agcaggccaacaagagccatgttgtcaagattcatgtctaatacttggtgtactccattgagacaagctcaaagattgttctcttctactactactatgcaaggttctggttctggttctatgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgctagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z