BBa_K2080001
1
BBa_K2080001
bax-sgRNA1
2016-09-21T11:00:00Z
2016-09-22T07:37:24Z
Form Bba_K2080001
This guide RNA is a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.
false
false
_2548_
32616
32616
9
false
We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.
false
Haojian Li
annotation2522688
1
gRNA scaffold
range2522688
1
285
367
annotation2522665
1
U6 promoter
range2522665
1
1
264
annotation2522668
1
gRNA for Bax promoter and Bax-1 promoter
range2522668
1
265
284
BBa_K2080001_sequence
1
aaggtcgggcaggaagagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccgtcatctataacgtcctgccgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z