BBa_K2080006
1
BBa_K2080006
bcl2-sgRNA3
2016-09-23T11:00:00Z
2016-09-24T08:48:20Z
BBa_K2080000
This guide RNA is a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2.
false
false
_2548_
0
32616
32616
9
It's complicated
false
We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bax trascription.
false
Haojian Li
annotation2523746
1
U6 promoter
range2523746
1
1
265
annotation2523812
1
gRNA
range2523812
1
266
285
annotation2523814
1
gRNA scaffold
range2523814
1
286
367
BBa_K2080006_sequence
1
aaggtcgggcaggaagagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccaccggagatctcaagagctcgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z