LOCUS       BBa_K2080009            1491 bp    DNA     linear       14-Nov-2024
DEFINITION  bax-sgRNA1234
ACCESSION   BBa_K2080009
VERSION     BBa_K2080009.1  
FEATURES             Location/Qualifiers
     promoter        1..264
                     /label=U6 promoter
     misc_feature    265..284
                     /label=gRNA for Bax promoter and Bax-1 promoter
     misc_feature    285..367
                     /label=gRNA scaffold 
     misc_feature    1..367
                     /label=BBa_K2080001
     promoter        376..640
                     /label=U6 promoter
     misc_feature    641..660
                     /label=gRNA
     misc_feature    661..742
                     /label=gRNA scaffold
     misc_feature    376..742
                     /label=BBa_K2080002
     promoter        751..1015
                     /label=U6 promoter
     misc_feature    1016..1034
                     /label=gRNA
     misc_feature    1035..1116
                     /label=misc
     misc_feature    751..1116
                     /label=BBa_K2080003
     promoter        1125..1389
                     /label=U6 promoter
     misc_feature    1125..1491
                     /label=BBa_K2080008
ORIGIN
        1 aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
       61 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
      121 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      181 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      241 atcttgtgga aaggacgaaa caccgtcatc tataacgtcc tgccgtttta gagctagaaa
      301 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      361 ttttttttac tagagaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat
      421 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca
      481 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
      541 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
      601 ttcttggctt tatatatctt gtggaaagga cgaaacaccg cgattggacg gacggctgtg
      661 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      721 gcaccgagtc ggtgcttttt tttactagag aaggtcgggc aggaagaggg cctatttccc
      781 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
      841 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      901 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      961 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggcag
     1021 cggccatttt gcggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     1081 aacttgaaaa agtggcaccg agtcggtgct tttttttact agagaaggtc gggcaggaag
     1141 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
     1201 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
     1261 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
     1321 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1381 gaaacaccgc tgatagatca gcagggtagt tttagagcta gaaatagcaa gttaaaataa
     1441 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt t
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