LOCUS       BBa_K2080010            1492 bp    DNA     linear       01-Jun-2024
DEFINITION  bcl2-sgRNA1234
ACCESSION   BBa_K2080010
VERSION     BBa_K2080010.1  
FEATURES             Location/Qualifiers
     promoter        1..265
                     /label=U6 promoter
     misc_feature    266..285
                     /label=gRNA
     misc_feature    286..367
                     /label=gRNA scaffold
     misc_feature    1..367
                     /label=BBa_K2080004
     promoter        376..640
                     /label=U6 promoter
     misc_feature    641..660
                     /label=gRNA
     misc_feature    661..742
                     /label=gRNA scaffold
     misc_feature    376..742
                     /label=BBa_K2080005
     promoter        751..1015
                     /label=U6 promoter
     misc_feature    1016..1035
                     /label=gRNA
     misc_feature    1036..1117
                     /label=gRNA scaffold
     misc_feature    751..1117
                     /label=BBa_K2080006
     promoter        1126..1390
                     /label=U6 promoter
     misc_feature    1391..1410
                     /label=gRNA 
     misc_feature    1411..1492
                     /label=gRNA scaffold
     misc_feature    1126..1492
                     /label=BBa_K2080007
ORIGIN
        1 aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
       61 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
      121 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      181 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      241 atcttgtgga aaggacgaaa caccaatgaa tcaggagtcg cggggtttta gagctagaaa
      301 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      361 ttttttttac tagagaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat
      421 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca
      481 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
      541 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
      601 ttcttggctt tatatatctt gtggaaagga cgaaacaccg atcgattccc agacttctgg
      661 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      721 gcaccgagtc ggtgcttttt tttactagag aaggtcgggc aggaagaggg cctatttccc
      781 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
      841 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      901 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      961 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccaccgga
     1021 gatctcaaga gctcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1081 caacttgaaa aagtggcacc gagtcggtgc ttttttttac tagagaaggt cgggcaggaa
     1141 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     1201 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     1261 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     1321 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     1381 cgaaacacca tcaatcttca gcactctccg ttttagagct agaaatagca agttaaaata
     1441 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tt
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