LOCUS       BBa_K208030             1051 bp    DNA     linear       01-Jun-2024
DEFINITION  GFP/HlyA/Terminator
ACCESSION   BBa_K208030
VERSION     BBa_K208030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start
     CDS             1..720
                     /label=GFP Coding Region
     CDS             1..720
                     /label=BBa_K208000
     misc_feature    729..914
                     /label=HlyA
     misc_feature    729..731
                     /label=Start
     misc_feature    729..914
                     /label=BBa_K208006
     stem_loop       934..977
                     /label=stem_loop
     misc_feature    923..1002
                     /label=BBa_B0010
     terminator      923..1002
                     /label=BBa_B0010
     stem_loop       1018..1037
                     /label=T7 TE
     misc_feature    1044..1044
                     /label=stop
     polyA_site      1038..1051
                     /label=polya
     misc_feature    1011..1051
                     /label=BBa_B0012
     terminator      1011..1051
                     /label=BBa_B0012
ORIGIN
        1 atggctagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
       61 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac
      121 ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      181 cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa
      241 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct
      301 ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt
      361 gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac
      421 aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat
      481 ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca
      541 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      601 tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
      661 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa
      721 tactagagtt agcctatgga agtcagggtg atcttaatcc attaattaat gaaatcagca
      781 aaatcatttc agcagcaggt agcttcgatg ttaaagagga aagaaccgca gcttctttat
      841 tgcagttgtc cggtaatgcc agtgattttt catatggacg gaactcaata accctgacca
      901 catcagcata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      961 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1021 tcaccttcgg gtgggccttt ctgcgtttat a
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