LOCUS       BBa_K208044             1011 bp    DNA     linear       25-Nov-2024
DEFINITION  Lac Promoter/RBS/OmpA/PhaP/Terminator
ACCESSION   BBa_K208044
VERSION     BBa_K208044.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..289
                     /label=OmpA
     misc_feature    227..229
                     /label=Start Codon
     misc_feature    227..289
                     /label=BBa_K208003
     misc_feature    296..298
                     /label=Start
     CDS             296..874
                     /label=Phasin
     CDS             296..874
                     /label=BBa_K208001
     stem_loop       894..937
                     /label=stem_loop
     misc_feature    883..962
                     /label=BBa_B0010
     terminator      883..962
                     /label=BBa_B0010
     stem_loop       978..997
                     /label=T7 TE
     misc_feature    1004..1004
                     /label=stop
     polyA_site      998..1011
                     /label=polya
     misc_feature    971..1011
                     /label=BBa_B0012
     terminator      971..1011
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaagacagc
      241 tatcgcgatt gcagtggcac tggctggttt cgctaccgta gcgcaggcct actagatgat
      301 cctcaccccg gaacaagttg cagcagcgca aaaggccaac ctcgaaacgc tgttcggcct
      361 gaccaccaag gcgtttgaag gcgtcgaaaa gctcgtcgag ctgaaccttc aggtcgtcaa
      421 gacttcgttc gcagaaggcg ttgacaacgc caagaaggcg ctgtcggcca aggacgcaca
      481 ggaactgctg gccatccagg ccgcagccgt gcagccggtt gccgaaaaga ccctggccta
      541 cacccgccac ctgtatgaaa tcgcttcgga aacccagagc gagttcacca aggtagccga
      601 ggctcaactg gccgaaggct cgaagaacgt gcaagcgctg gtcgagaacc tcgccaagaa
      661 cgccccggcc ggttcggaat cgaccgtggc catcgtgaag tcggcgatct ccgctgccaa
      721 caacgcctac gagtcggtgc agaaggcgac caagcaagcg gtcgaaatcg ctgaaaccaa
      781 cttccaggct gcggctacgg ctgccaccaa ggctgcccag caagccagcg ccacggcccg
      841 tacggccacg gcaaagaaga cgacggctgc ctgatactag agccaggcat caaataaaac
      901 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      961 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//