LOCUS       BBa_K208045             1083 bp    DNA     linear       22-Nov-2024
DEFINITION  Lac Promoter/RBS/GFP/Term
ACCESSION   BBa_K208045
VERSION     BBa_K208045.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start
     CDS             227..946
                     /label=GFP Coding Region
     CDS             227..946
                     /label=BBa_K208000
     stem_loop       966..1009
                     /label=stem_loop
     misc_feature    955..1034
                     /label=BBa_B0010
     terminator      955..1034
                     /label=BBa_B0010
     stem_loop       1050..1069
                     /label=T7 TE
     misc_feature    1076..1076
                     /label=stop
     polyA_site      1070..1083
                     /label=polya
     misc_feature    1043..1083
                     /label=BBa_B0012
     terminator      1043..1083
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctagcaaagg
      241 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      301 gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa agcttaccct
      361 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      421 ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt
      481 caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg
      541 gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga
      601 gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac tcgagtacaa
      661 ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa
      721 cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc attatcaaca
      781 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca
      841 atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt
      901 aactgctgct gggattacac atggcatgga tgagctctac aaataatact agagccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1081 ata
//