LOCUS       BBa_K208046             1155 bp    DNA     linear       26-Dec-2024
DEFINITION  Lac Promoter/RBS/PelB/GFP(Hawaii08 Silver)/Term
ACCESSION   BBa_K208046
VERSION     BBa_K208046.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..292
                     /label=PelB
     misc_feature    227..229
                     /label=Start
     misc_feature    227..292
                     /label=BBa_K208004
     CDS             301..1018
                     /label=GFP fusion brick
     misc_feature    301..301
                     /label=deletion of TG
     CDS             301..1018
                     /label=BBa_K125500
     stem_loop       1038..1081
                     /label=stem_loop
     misc_feature    1027..1106
                     /label=BBa_B0010
     terminator      1027..1106
                     /label=BBa_B0010
     stem_loop       1122..1141
                     /label=T7 TE
     misc_feature    1148..1148
                     /label=stop
     polyA_site      1142..1155
                     /label=polya
     misc_feature    1115..1155
                     /label=BBa_B0012
     terminator      1115..1155
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatacctgct
      241 gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg cctactagag
      301 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      361 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      421 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      481 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
      541 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      601 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      661 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      721 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
      781 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
      841 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
      901 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
      961 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatta
     1021 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1081 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1141 ctttctgcgt ttata
//