LOCUS       BBa_K208047             1152 bp    DNA     linear       22-Nov-2024
DEFINITION  Lac Promoter/RBS/OmpA/GFP(Hawaii08 Silver)/Term
ACCESSION   BBa_K208047
VERSION     BBa_K208047.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..289
                     /label=OmpA
     misc_feature    227..229
                     /label=Start Codon
     misc_feature    227..289
                     /label=BBa_K208003
     CDS             298..1015
                     /label=GFP fusion brick
     misc_feature    298..298
                     /label=deletion of TG
     CDS             298..1015
                     /label=BBa_K125500
     stem_loop       1035..1078
                     /label=stem_loop
     misc_feature    1024..1103
                     /label=BBa_B0010
     terminator      1024..1103
                     /label=BBa_B0010
     stem_loop       1119..1138
                     /label=T7 TE
     misc_feature    1145..1145
                     /label=stop
     polyA_site      1139..1152
                     /label=polya
     misc_feature    1112..1152
                     /label=BBa_B0012
     terminator      1112..1152
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaagacagc
      241 tatcgcgatt gcagtggcac tggctggttt cgctaccgta gcgcaggcct actagagcgt
      301 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      361 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      421 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      481 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      541 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      601 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      661 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      721 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      781 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
      841 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
      901 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
      961 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataattacta
     1021 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1081 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1141 tctgcgttta ta
//