LOCUS       BBa_K208048             1074 bp    DNA     linear       22-Dec-2024
DEFINITION  Lac Promoter/RBS/TorA/Phasin/Term
ACCESSION   BBa_K208048
VERSION     BBa_K208048.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..352
                     /label=TorA
     misc_feature    227..229
                     /label=Start
     misc_feature    227..352
                     /label=BBa_K208005
     misc_feature    359..361
                     /label=Start
     CDS             359..937
                     /label=Phasin
     CDS             359..937
                     /label=BBa_K208001
     stem_loop       957..1000
                     /label=stem_loop
     misc_feature    946..1025
                     /label=BBa_B0010
     terminator      946..1025
                     /label=BBa_B0010
     stem_loop       1041..1060
                     /label=T7 TE
     misc_feature    1067..1067
                     /label=stop
     polyA_site      1061..1074
                     /label=polya
     misc_feature    1034..1074
                     /label=BBa_B0012
     terminator      1034..1074
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acaataacga
      241 tctctttcag gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa ccgtcgccgg
      301 gatgctgggg ccgtcattgt taacgccgcg acgtgcgact gcggcgcaag cgtactagat
      361 gatcctcacc ccggaacaag ttgcagcagc gcaaaaggcc aacctcgaaa cgctgttcgg
      421 cctgaccacc aaggcgtttg aaggcgtcga aaagctcgtc gagctgaacc ttcaggtcgt
      481 caagacttcg ttcgcagaag gcgttgacaa cgccaagaag gcgctgtcgg ccaaggacgc
      541 acaggaactg ctggccatcc aggccgcagc cgtgcagccg gttgccgaaa agaccctggc
      601 ctacacccgc cacctgtatg aaatcgcttc ggaaacccag agcgagttca ccaaggtagc
      661 cgaggctcaa ctggccgaag gctcgaagaa cgtgcaagcg ctggtcgaga acctcgccaa
      721 gaacgccccg gccggttcgg aatcgaccgt ggccatcgtg aagtcggcga tctccgctgc
      781 caacaacgcc tacgagtcgg tgcagaaggc gaccaagcaa gcggtcgaaa tcgctgaaac
      841 caacttccag gctgcggcta cggctgccac caaggctgcc cagcaagcca gcgccacggc
      901 ccgtacggcc acggcaaaga agacgacggc tgcctgatac tagagccagg catcaaataa
      961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//