LOCUS       BBa_K208050              928 bp    DNA     linear       23-Dec-2024
DEFINITION  Tet Promoter/RBS/TorA/Phasin/Terminator
ACCESSION   BBa_K208050
VERSION     BBa_K208050.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..206
                     /label=TorA
     misc_feature    81..83
                     /label=Start
     misc_feature    81..206
                     /label=BBa_K208005
     misc_feature    213..215
                     /label=Start
     CDS             213..791
                     /label=Phasin
     CDS             213..791
                     /label=BBa_K208001
     stem_loop       811..854
                     /label=stem_loop
     misc_feature    800..879
                     /label=BBa_B0010
     terminator      800..879
                     /label=BBa_B0010
     stem_loop       895..914
                     /label=T7 TE
     misc_feature    921..921
                     /label=stop
     polyA_site      915..928
                     /label=polya
     misc_feature    888..928
                     /label=BBa_B0012
     terminator      888..928
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaacaata acgatctctt tcaggcatca cgtcggcgtt
      121 ttctggcaca actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc
      181 cgcgacgtgc gactgcggcg caagcgtact agatgatcct caccccggaa caagttgcag
      241 cagcgcaaaa ggccaacctc gaaacgctgt tcggcctgac caccaaggcg tttgaaggcg
      301 tcgaaaagct cgtcgagctg aaccttcagg tcgtcaagac ttcgttcgca gaaggcgttg
      361 acaacgccaa gaaggcgctg tcggccaagg acgcacagga actgctggcc atccaggccg
      421 cagccgtgca gccggttgcc gaaaagaccc tggcctacac ccgccacctg tatgaaatcg
      481 cttcggaaac ccagagcgag ttcaccaagg tagccgaggc tcaactggcc gaaggctcga
      541 agaacgtgca agcgctggtc gagaacctcg ccaagaacgc cccggccggt tcggaatcga
      601 ccgtggccat cgtgaagtcg gcgatctccg ctgccaacaa cgcctacgag tcggtgcaga
      661 aggcgaccaa gcaagcggtc gaaatcgctg aaaccaactt ccaggctgcg gctacggctg
      721 ccaccaaggc tgcccagcaa gccagcgcca cggcccgtac ggccacggca aagaagacga
      781 cggctgcctg atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      841 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      901 ccttcgggtg ggcctttctg cgtttata
//