LOCUS       BBa_K208051             1069 bp    DNA     linear       25-Nov-2024
DEFINITION  Tet Promoter/RBS/TorA/GFP/Terminator
ACCESSION   BBa_K208051
VERSION     BBa_K208051.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..206
                     /label=TorA
     misc_feature    81..83
                     /label=Start
     misc_feature    81..206
                     /label=BBa_K208005
     misc_feature    213..215
                     /label=Start
     CDS             213..932
                     /label=GFP Coding Region
     CDS             213..932
                     /label=BBa_K208000
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaacaata acgatctctt tcaggcatca cgtcggcgtt
      121 ttctggcaca actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc
      181 cgcgacgtgc gactgcggcg caagcgtact agatggctag caaaggagaa gaacttttca
      241 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      301 tcagtggaga gggtgaaggt gatgctacat acggaaagct tacccttaaa tttatttgca
      361 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttctct tatggtgttc
      421 aatgcttttc ccgttatccg gatcatatga aacggcatga ctttttcaag agtgccatgc
      481 ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggaac tacaagacgc
      541 gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta aaaggtattg
      601 attttaaaga agatggaaac attctcggac acaaactcga gtacaactat aactcacaca
      661 atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc aaaattcgcc
      721 acaacattga agatggatcc gttcaactag cagaccatta tcaacaaaat actccaattg
      781 gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct gccctttcga
      841 aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact gctgctggga
      901 ttacacatgg catggatgag ctctacaaat aatactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//