LOCUS BBa_K2082117 5049 bp DNA linear 22-Dec-2024 DEFINITION Six mutator genes under control of a modified Pbad ACCESSION BBa_K2082117 VERSION BBa_K2082117.1 FEATURES Location/Qualifiers CDS 1..879 /label=AraC misc_feature 909..924 /label=AraO misc_feature 1066..1077 /label=AraO1 promoter 1153..1187 /label=Pbad(modified) promoter 1..1209 /label=BBa_K2082112 misc_feature 1218..1231 /label=BBa_B0031 misc_feature 1238..1969 /label=BBa_K1333108 misc_feature 1973..1986 /label=BBa_B0031 misc_feature 1993..2829 /label=BBa_K1500994 misc_feature 2833..2846 /label=BBa_B0031 misc_feature 2855..3398 /label=BBa_K2082124 misc_feature 3410..3423 /label=BBa_B0031 misc_feature 3430..3960 /label=BBa_K1500995 misc_feature 3964..3977 /label=BBa_B0031 misc_feature 3984..4238 /label=BBa_K2082104 misc_feature 4242..4255 /label=BBa_B0031 misc_feature 4262..4888 /label=BBa_K2082105 misc_feature 4922..5001 /label=BBa_B0010 misc_feature 5017..5036 /label=BBa_B0012 misc_feature 1218..5049 /label=BBa_K2082111 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgcc ccccccgtcc accccctaat atcccgacat acccgttttt 1201 tgggctaact actagagtca cacaggaaac ctactagatg agcactgcaa ttacacgcca 1261 gatcgttctc gctaccgcaa ccaccggtat gaaccagatt ggtgcgcact atgaaggcca 1321 caagatcatt gagattggtg ccgttgaagt ggtgaaccgt cgcctgacgg gcaataactt 1381 ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg tacatggtat 1441 tgccgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg agttcatgga 1501 ctatattcgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatcg gctttatgga 1561 ctacgagttt tcgttgctta agcgcgatat tccgaagacc aatactttct gtaaggtcac 1621 cgatagcctt gcggtggcga ggaaaatgtt tcccggtaag cgcaacagcc tcgatgcgtt 1681 atgtgctcgc tacgaaatag ataacagtaa acgaacgctg cacggggcat tactcgatgc 1741 ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga tggcttttgc 1801 gatggaagga gagacacaac agcaacaagg tgaagcaaca attcagcgca ttgtacgtca 1861 ggcaagtaag ttacgcgttg tttttgcgac agatgaagag attgcagctc atgaagcccg 1921 tctcgatctg gtgcagaaga aaggcggaag ttgcctctgg cgagcataat aatcacacag 1981 gaaacctact agatgaagaa aaatcgcgct tttttgaagt gggcaggggg caagtatccc 2041 ctgcttgatg atattaaacg gcatttgccc aagggcgaat gtctggttga gccttttgta 2101 ggtgccgggt cggtgtttct caacaccgac ttttctcgtt atatccttgc cgatatcaat 2161 agcgacctga tcagtctcta taacattgtg aagatgcgta ctgatgagta cgtacaggcc 2221 gcacgcgagc tgtttgttcc cgaaacaaat tgcgccgagg tttactatca gttccgcgaa 2281 gagttcaaca aaagccagga tccgttccgt cgggcggtac tgtttttata tttgaaccgc 2341 tacggttaca acggcctgtg tcgttacaat ctgcgcggtg agtttaacgt gccgttcggc 2401 cgctacaaaa aaccctattt cccggaagca gagttgtatc acttcgctga aaaagcgcag 2461 aatgcctttt tctattgtga gtcttacgcc gatagcatgg cgcgcgcaga tgatgcatcc 2521 gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt tacggcgtat 2581 cacacaaaca gttttacgct tgaacaacaa gcgcatctgg cggagatcgc cgaaggtctg 2641 gttgagcgcc atattccagt gctgatctcc aatcacgata cgatgttaac gcgtgagtgg 2701 tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca gtataagcag caacggcggc 2761 acacgtaaaa aggtggacga actgctggct ttgtacaaac caggagtcgt ttcacccgcg 2821 aaaaaataat aatcacacag gaaacctact agatgaaaac gattgaagtt gatgatgaac 2881 tctacagcta tattgccagc cacactaagc atatcggcga gagcgcatcc gacattttac 2941 ggcgtatgtt gaaattttcc gccgcatcac agcctgctgc tccggtgacg aaagaggttc 3001 gcgttgcgtc acctgctatc gtcgaagcga agccggtcaa aacgattaaa gacaaggttc 3061 gcgcaatgcg tgaacttctg ctttcggatg aatacgcaga gcaaaagcga gcggtcaatc 3121 gctttatgct gctgttgtct acactatatt ctcttgacgc ccaggcgttt gccgaagcaa 3181 cggaatcgtt gcacggtcgt acacgcgttt actttgcggc agatgaacaa acgctgctga 3241 aaaatggtaa tcagaccaag ccgaaacatg tgccaggcac gccgtattgg gtgatcacca 3301 acaccaacac cggccgtaaa tgcagcatga tcgaacacat catgcagtcg atgcaattcc 3361 cggcggaatt gattgagaag gtttgcggaa ctatctaata atactagagt cacacaggaa 3421 acctactaga tggatagttc gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc 3481 cgccacgaag attttcctta tcaggagatc cttctgactc gtctttgcat gcacatgcaa 3541 agcaagctgc tggagaaccg caataaaatg ctgaaggctc agggaattaa cgagacgttg 3601 tttatggcgt tgattacgct ggagtctcag gaaaaccaca gtattcagcc ttctgaatta 3661 agttgtgctc ttggatcatc ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa 3721 cgcggttgga tcgaacgtcg tgaaagcgat aacgatcgcc gctgcctgca tctgcaatta 3781 acggaaaaag gtcacgagtt tttgcgcgag gttttaccac cgcagcataa ctgcctgcat 3841 caactctggt ccgcgctcag cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa 3901 ttgctctccc gtctcgacca gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa 3961 taatcacaca ggaaacctac tagatgacaa atttatctga catcattgaa aaagaaacag 4021 gaaaacaact cgtgattcaa gaatcaattc taatgttacc agaagaagta gaggaagtaa 4081 ttgggaataa accagaaagt gatattttag ttcatactgc ttatgatgaa agtacagatg 4141 aaaatgtaat gctattaact tcagatgctc cagaatataa accttgggct ttagtaattc 4201 aagacagtaa tggagaaaat aaaattaaaa tgttataata atcacacagg aaacctacta 4261 gatgaccgac gcggaatacg ttcgtatcca cgaaaaactg gacatctaca ccttcaaaaa 4321 acagttcttc aacaacaaaa aatctgtttc tcaccgttgc tacgttctgt tcgaactgaa 4381 acgtcgtggt gaacgtcgtg cgtgcttctg gggttacgcg gttaacaaac cgcagtctgg 4441 taccgaacgt ggtatccacg cggaaatctt ctctatccgt aaagttgaag aatacctgcg 4501 tgacaacccg ggtcagttca ccatcaactg gtactcttct tggtctccgt gcgcggactg 4561 cgcggaaaaa atcctggaat ggtacaacca ggaactgcgt ggtaacggtc acaccctgaa 4621 aatctgggcg tgcaaactgt actacgaaaa aaacgcgcgt aaccagatcg gtctgtggaa 4681 cctgcgtgac aacggtgttg gtctgaacgt tatggtttct gaacactacc agtgctgccg 4741 taaaatcttc atccagtctt ctcacaacca gctgaacgaa aaccgttggc tggaaaaaac 4801 cctgaaacgt gcggaaaaac gtcgttctga actgtctatc atgatccagg ttaaaatcct 4861 gcacaccacc aaatctccgg cggtttaacg ctgatagtgc tagtgtagat cgctactaga 4921 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 4981 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 5041 tgcgtttat //