LOCUS       BBa_K2082224             717 bp    DNA     linear       31-Dec-2025
DEFINITION  Double mutated fusion protein HA4(R38A,E52A):RpoZ generator
ACCESSION   BBa_K2082224
VERSION     BBa_K2082224.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..334
                     /label=rpoZ
     misc_feature    335..365
                     /label=cMyc
     CDS             366..670
                     /label=monobody HA4
     misc_feature    476..478
                     /label=R38A
     misc_feature    518..520
                     /label=E52A
     CDS             62..670
                     /label=BBa_K2082204
     misc_feature    679..717
                     /label=B1006
     stem_loop       688..709
                     /label=modified thr terminator
     polyA_site      710..717
                     /label=PolyA
     polyA_site      679..687
                     /label=PolyA
     terminator      679..717
                     /label=BBa_B1006
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggcacgc gtaactgttc aggacgctgt agagaaaatt ggtaaccgtt ttgacctggt
      121 actggtcgcc gcgcgtcgcg ctcgtcagat gcaggtaggc ggaaaggatc cgctggtacc
      181 ggaagaaaac gataaaacca ctgtaatcgc gctgcgcgaa atcgaagaag gtctgatcaa
      241 caaccagatc ctcgacgttc gcgaacgcca ggaacagcaa gagcaggaag ccgctgaatt
      301 acaagccgtt accgctattg ctgaaggtcg tcgtgaacaa aagcttattt ctgaagagga
      361 cttgggcagc agcgtgagca gcgtgccgac caaactggaa gtggtggcgg cgaccccgac
      421 cagcctgctg attagctggg atgcgccgat gagcagcagc agcgtgtatt attatgcgat
      481 tacctatggc gaaaccggcg gcaacagccc ggtgcaggcg tttaccgtgc cgtatagcag
      541 cagcaccgcg accattagcg gcctgagccc gggcgtggat tataccatta ccgtgtatgc
      601 gtggggcgaa gatagcgcgg gctatatgtt tatgtatagc ccgattagca ttaactatcg
      661 cacctgctaa tactagagaa aaaaaaaccc cgcccctgac agggcggggt ttttttt
//