LOCUS       BBa_K2082232            1533 bp    DNA     linear       18-May-2024
DEFINITION  RFP under the control of an optimized lacZ promoter combined with 
            Gal11P:cI(434)
ACCESSION   BBa_K2082232
VERSION     BBa_K2082232.1  
FEATURES             Location/Qualifiers
     misc_feature    4..17
                     /label=OR1 binding site of cI (434)
     promoter        19..75
                     /label=optimized lacZ promotor
     promoter        1..75
                     /label=BBa_K2082210
     misc_feature    88..91
                     /label=conserved
     RBS             84..95
                     /label=BBa_B0034
     CDS             102..779
                     /label=RFP
     CDS             102..779
                     /label=BBa_K2082291
     misc_feature    788..826
                     /label=B1006
     stem_loop       797..818
                     /label=modified thr terminator
     polyA_site      819..826
                     /label=PolyA
     polyA_site      788..796
                     /label=PolyA
     terminator      788..826
                     /label=BBa_B1006
     misc_feature    1..826
                     /label=BBa_K2082211
     promoter        835..869
                     /label=BBa_J23106
     misc_feature    882..885
                     /label=conserved
     RBS             878..889
                     /label=BBa_B0034
     CDS             896..1180
                     /label=434 cI
     misc_feature    1181..1207
                     /label=9-aa-linker
     CDS             1208..1486
                     /label=Gal11P
     CDS             896..1486
                     /label=BBa_K2082209
     misc_feature    1495..1533
                     /label=B1006
     stem_loop       1504..1525
                     /label=modified thr terminator
     polyA_site      1526..1533
                     /label=PolyA
     polyA_site      1495..1503
                     /label=PolyA
     terminator      1495..1533
                     /label=BBa_B1006
     misc_feature    835..1533
                     /label=BBa_K2082227
ORIGIN
        1 aatacaagaa agtttgttca ttaggcaccc cgggctttac tcgtaaagct tccggcgcgt
       61 atgttgtgtc gaccgtacta gagaaagagg agaaatacta gatggcttcc tccgaagacg
      121 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
      181 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
      241 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
      301 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
      361 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
      421 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
      481 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
      541 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
      601 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
      661 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
      721 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
      781 actagagaaa aaaaaacccc gcccctgaca gggcggggtt tttttttact agagtttacg
      841 gctagctcag tcctaggtat agtgctagct actagagaaa gaggagaaat actagatgag
      901 tatttcttcc agggtaaaaa gcaaaaggat tcagcttgga cttaaccagg ctgaacttgc
      961 tcaaaaggtg gggactaccc agcagtctat agagcagctc gaaaacggta aaactaagcg
     1021 accacgcttt ttaccagaac ttgcgtcagc tcttggcgta agtgttgact ggctgctcaa
     1081 tggcacctct gattcgaatg ttagatttgt tgggcacgtt gagcccaaag ggaaatatcc
     1141 attgattagc atggttagag ctggttcgtg gtgtgaagct gcggcggcgc caagagtcag
     1201 aactggtaac attccgcagc agcagcagat gcagccgccg aacagcagcg cgaacaacaa
     1261 cccgctacag cagcagagca gccagaacac cgtgccgaac gtgctgaacc agattaacca
     1321 gatttttagc ccggaagaac agcgcagcct gctacaggaa gcgattgaaa cctgcaaaaa
     1381 ctttgaaaaa acccagctgg gcagcaccat gaccgaaccg gtgaaacaga gctttattcg
     1441 caaatatatt gcgcagaaag cgctgcgcaa aattcaggcg ctgtaatact agagaaaaaa
     1501 aaaccccgcc cctgacaggg cggggttttt ttt
//