BBa_K2082211
1
BBa_K2082211
RFP under the control of an optimized lacZ promoter
2016-10-07T11:00:00Z
2016-10-21T04:09:39Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
component2503284
1
BBa_K2082291
component2503282
1
BBa_B0034
component2503280
1
BBa_K2082210
component2503289
1
BBa_B1006
annotation2503280
1
BBa_K2082210
range2503280
1
1
75
annotation2503284
1
BBa_K2082291
range2503284
1
102
779
annotation2503289
1
BBa_B1006
range2503289
1
788
826
annotation2503282
1
BBa_B0034
range2503282
1
84
95
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K2082291
1
BBa_K2082291
Red fluorescence protein
2016-10-13T11:00:00Z
2016-10-14T07:00:45Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
annotation2503254
1
RFP
range2503254
1
1
678
BBa_K2082227
1
BBa_K2082227
Fusion protein Gal11P:cI (434) generator
2016-10-07T11:00:00Z
2016-10-21T04:37:25Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
component2487683
1
BBa_B0034
component2487681
1
BBa_J23106
component2487684
1
BBa_K2082209
component2487689
1
BBa_B1006
annotation2487681
1
BBa_J23106
range2487681
1
1
35
annotation2487684
1
BBa_K2082209
range2487684
1
62
652
annotation2487683
1
BBa_B0034
range2487683
1
44
55
annotation2487689
1
BBa_B1006
range2487689
1
661
699
BBa_K2082209
1
BBa_K2082209
Fusion protein Gal11P:cI(434)
2016-10-06T11:00:00Z
2016-10-20T08:00:19Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
annotation2526030
1
Gal11P
range2526030
1
313
591
annotation2526027
1
434 cI
range2526027
1
1
285
annotation2526028
1
9-aa-linker
range2526028
1
286
312
BBa_K2082210
1
BBa_K2082210
Optimized lacZ promoter with binding site of regulatory protein cI of phage 434
2016-10-07T11:00:00Z
2016-10-21T03:27:05Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
annotation2487690
1
OR1 binding site of cI (434)
range2487690
1
4
17
annotation2487691
1
optimized lacZ promotor
range2487691
1
19
75
BBa_K2082232
1
BBa_K2082232
RFP under the control of an optimized lacZ promoter combined with Gal11P:cI(434)
2016-10-07T11:00:00Z
2016-10-21T04:40:26Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
component2503363
1
BBa_K2082211
component2503373
1
BBa_K2082227
annotation2503363
1
BBa_K2082211
range2503363
1
1
826
annotation2503373
1
BBa_K2082227
range2503373
1
835
1533
BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898429
1
modified thr terminator
range1898429
1
10
31
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
BBa_J23106
1
BBa_J23106
constitutive promoter family member
2006-08-13T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K2082232_sequence
1
aatacaagaaagtttgttcattaggcaccccgggctttactcgtaaagcttccggcgcgtatgttgtgtcgaccgtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagtttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaaggattcagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagctgcggcggcgccaagagtcagaactggtaacattccgcagcagcagcagatgcagccgccgaacagcagcgcgaacaacaacccgctacagcagcagagcagccagaacaccgtgccgaacgtgctgaaccagattaaccagatttttagcccggaagaacagcgcagcctgctacaggaagcgattgaaacctgcaaaaactttgaaaaaacccagctgggcagcaccatgaccgaaccggtgaaacagagctttattcgcaaatatattgcgcagaaagcgctgcgcaaaattcaggcgctgtaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2082227_sequence
1
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaaggattcagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagctgcggcggcgccaagagtcagaactggtaacattccgcagcagcagcagatgcagccgccgaacagcagcgcgaacaacaacccgctacagcagcagagcagccagaacaccgtgccgaacgtgctgaaccagattaaccagatttttagcccggaagaacagcgcagcctgctacaggaagcgattgaaacctgcaaaaactttgaaaaaacccagctgggcagcaccatgaccgaaccggtgaaacagagctttattcgcaaatatattgcgcagaaagcgctgcgcaaaattcaggcgctgtaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K2082210_sequence
1
aatacaagaaagtttgttcattaggcaccccgggctttactcgtaaagcttccggcgcgtatgttgtgtcgaccg
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_J23106_sequence
1
tttacggctagctcagtcctaggtatagtgctagc
BBa_K2082291_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaa
BBa_K2082211_sequence
1
aatacaagaaagtttgttcattaggcaccccgggctttactcgtaaagcttccggcgcgtatgttgtgtcgaccgtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K2082209_sequence
1
atgagtatttcttccagggtaaaaagcaaaaggattcagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagctgcggcggcgccaagagtcagaactggtaacattccgcagcagcagcagatgcagccgccgaacagcagcgcgaacaacaacccgctacagcagcagagcagccagaacaccgtgccgaacgtgctgaaccagattaaccagatttttagcccggaagaacagcgcagcctgctacaggaagcgattgaaacctgcaaaaactttgaaaaaacccagctgggcagcaccatgaccgaaccggtgaaacagagctttattcgcaaatatattgcgcagaaagcgctgcgcaaaattcaggcgctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z