LOCUS       BBa_K2082237            2813 bp    DNA     linear       22-Nov-2024
DEFINITION  RFP and Tetracycline resistence(tetA) under the control of a 
            modified lacZ promoter; Fusion protein
ACCESSION   BBa_K2082237
VERSION     BBa_K2082237.1  
FEATURES             Location/Qualifiers
     misc_feature    4..17
                     /label=OR1 binding site of cI (434)
     promoter        19..75
                     /label=optimized lacZ promotor
     promoter        1..75
                     /label=BBa_K2082210
     misc_feature    88..91
                     /label=conserved
     RBS             84..95
                     /label=BBa_B0034
     CDS             102..1292
                     /label=tetracycline reistance 
     misc_feature    905..905
                     /label=C804T
     misc_feature    1150..1150
                     /label=T1049C
     CDS             102..1292
                     /label=BBa_K2082290
     misc_feature    1305..1308
                     /label=conserved
     RBS             1301..1312
                     /label=BBa_B0034
     CDS             1319..1996
                     /label=RFP
     CDS             1319..1996
                     /label=BBa_K2082291
     misc_feature    2005..2043
                     /label=B1006
     stem_loop       2014..2035
                     /label=modified thr terminator
     polyA_site      2036..2043
                     /label=PolyA
     polyA_site      2005..2013
                     /label=PolyA
     terminator      2005..2043
                     /label=BBa_B1006
     promoter        2052..2086
                     /label=BBa_J23106
     misc_feature    2099..2102
                     /label=conserved
     RBS             2095..2106
                     /label=BBa_B0034
     CDS             2113..2397
                     /label=434 cI
     misc_feature    2398..2427
                     /label=cMyc linker
     CDS             2428..2766
                     /label=SH2 domain of Abl1
     CDS             2113..2766
                     /label=BBa_K2082207
     misc_feature    2775..2813
                     /label=B1006
     stem_loop       2784..2805
                     /label=modified thr terminator
     polyA_site      2806..2813
                     /label=PolyA
     polyA_site      2775..2783
                     /label=PolyA
     terminator      2775..2813
                     /label=BBa_B1006
     misc_feature    2052..2813
                     /label=BBa_K2082225
ORIGIN
        1 aatacaagaa agtttgttca ttaggcaccc cgggctttac tcgtaaagct tccggcgcgt
       61 atgttgtgtc gaccgtacta gagaaagagg agaaatacta gatgaaatct aacaatgcgc
      121 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg
      181 tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg
      241 tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt
      301 ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact
      361 acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg
      421 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
      481 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
      541 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
      601 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
      661 gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
      721 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
      781 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
      841 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
      901 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
      961 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     1021 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     1081 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa
     1141 cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga
     1201 acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc
     1261 gcggtgcatg gagccgggcc acctcgacct aatactagag aaagaggaga aatactagat
     1321 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
     1381 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
     1441 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
     1501 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
     1561 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
     1621 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
     1681 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
     1741 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat
     1801 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
     1861 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
     1921 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
     1981 ctccaccggt gcttaatact agagaaaaaa aaaccccgcc cctgacaggg cggggttttt
     2041 ttttactaga gtttacggct agctcagtcc taggtatagt gctagctact agagaaagag
     2101 gagaaatact agatgagtat ttcttccagg gtaaaaagca aaaggattca gcttggactt
     2161 aaccaggctg aacttgctca aaaggtgggg actacccagc agtctataga gcagctcgaa
     2221 aacggtaaaa ctaagcgacc acgcttttta ccagaacttg cgtcagctct tggcgtaagt
     2281 gttgactggc tgctcaatgg cacctctgat tcgaatgtta gatttgttgg gcacgttgag
     2341 cccaaaggga aatatccatt gattagcatg gttagagctg gttcgtggtg tgaagctgaa
     2401 caaaagctta tttctgaaga ggacttgagc ctggaaaaac atagctggta tcatggcccg
     2461 gtgagccgca acgcggcgga atatctgctg agcagcggca ttaacggcag ctttctggtg
     2521 cgcgaaagcg aaagcagccc gggccagcgc agcattagcc tgcgctatga aggccgcgtg
     2581 tatcattatc gcattaacac cgcgagcgat ggcaaactgt atgtgagcag cgaaagccgc
     2641 tttaacaccc tggcggaact ggtgcatcat catagcaccg tggcggatgg cctgattacc
     2701 accctgcatt atccggcgcc gaaacgcaac aaaccgaccg tgtatggcgt gagcccgaac
     2761 tattaatact agagaaaaaa aaaccccgcc cctgacaggg cggggttttt ttt
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