BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K2082290
1
BBa_K2082290
Tetracycline resistance
2016-10-13T11:00:00Z
2016-10-14T06:39:58Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
annotation2503037
1
T1049C
range2503037
1
1049
1049
annotation2502964
1
tetracycline reistance
range2502964
1
1
1191
annotation2503002
1
C804T
range2503002
1
804
804
BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898429
1
modified thr terminator
range1898429
1
10
31
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
BBa_K2082237
1
BBa_K2082237
RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein
2016-10-13T11:00:00Z
2016-10-21T04:49:13Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
component2503420
1
BBa_K2082225
component2503392
1
BBa_K2082210
component2503398
1
BBa_K2082290
component2503402
1
BBa_K2082291
component2503407
1
BBa_B1006
component2503400
1
BBa_B0034
component2503394
1
BBa_B0034
annotation2503402
1
BBa_K2082291
range2503402
1
1319
1996
annotation2503420
1
BBa_K2082225
range2503420
1
2052
2813
annotation2503398
1
BBa_K2082290
range2503398
1
102
1292
annotation2503392
1
BBa_K2082210
range2503392
1
1
75
annotation2503407
1
BBa_B1006
range2503407
1
2005
2043
annotation2503394
1
BBa_B0034
range2503394
1
84
95
annotation2503400
1
BBa_B0034
range2503400
1
1301
1312
BBa_K2082207
1
BBa_K2082207
Fusion protein SH2:cMyc:cI(434)
2016-10-06T11:00:00Z
2016-10-20T07:18:28Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
annotation2487554
1
434 cI
range2487554
1
1
285
annotation2487555
1
cMyc linker
range2487555
1
286
315
annotation2487556
1
SH2 domain of Abl1
range2487556
1
316
654
BBa_K2082210
1
BBa_K2082210
Optimized lacZ promoter with binding site of regulatory protein cI of phage 434
2016-10-07T11:00:00Z
2016-10-21T03:27:05Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
annotation2487690
1
OR1 binding site of cI (434)
range2487690
1
4
17
annotation2487691
1
optimized lacZ promotor
range2487691
1
19
75
BBa_K2082291
1
BBa_K2082291
Red fluorescence protein
2016-10-13T11:00:00Z
2016-10-14T07:00:45Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
false
Coming soon
false
Pascal Schmidt
annotation2503254
1
RFP
range2503254
1
1
678
BBa_J23106
1
BBa_J23106
constitutive promoter family member
2006-08-13T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K2082225
1
BBa_K2082225
Fusion protein SH2:cMyc:cI(434) generator
2016-10-07T11:00:00Z
2016-10-21T04:31:34Z
Coming soon
Coming soon
false
false
_2550_
30782
30782
9
true
Coming soon
false
Pascal Schmidt
component2487650
1
BBa_B0034
component2487648
1
BBa_J23106
component2487654
1
BBa_K2082207
component2487659
1
BBa_B1006
annotation2487650
1
BBa_B0034
range2487650
1
44
55
annotation2487654
1
BBa_K2082207
range2487654
1
62
715
annotation2487659
1
BBa_B1006
range2487659
1
724
762
annotation2487648
1
BBa_J23106
range2487648
1
1
35
BBa_K2082290_sequence
1
atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2082210_sequence
1
aatacaagaaagtttgttcattaggcaccccgggctttactcgtaaagcttccggcgcgtatgttgtgtcgaccg
BBa_K2082237_sequence
1
aatacaagaaagtttgttcattaggcaccccgggctttactcgtaaagcttccggcgcgtatgttgtgtcgaccgtactagagaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagtttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaaggattcagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagctgaacaaaagcttatttctgaagaggacttgagcctggaaaaacatagctggtatcatggcccggtgagccgcaacgcggcggaatatctgctgagcagcggcattaacggcagctttctggtgcgcgaaagcgaaagcagcccgggccagcgcagcattagcctgcgctatgaaggccgcgtgtatcattatcgcattaacaccgcgagcgatggcaaactgtatgtgagcagcgaaagccgctttaacaccctggcggaactggtgcatcatcatagcaccgtggcggatggcctgattaccaccctgcattatccggcgccgaaacgcaacaaaccgaccgtgtatggcgtgagcccgaactattaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K2082207_sequence
1
atgagtatttcttccagggtaaaaagcaaaaggattcagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagctgaacaaaagcttatttctgaagaggacttgagcctggaaaaacatagctggtatcatggcccggtgagccgcaacgcggcggaatatctgctgagcagcggcattaacggcagctttctggtgcgcgaaagcgaaagcagcccgggccagcgcagcattagcctgcgctatgaaggccgcgtgtatcattatcgcattaacaccgcgagcgatggcaaactgtatgtgagcagcgaaagccgctttaacaccctggcggaactggtgcatcatcatagcaccgtggcggatggcctgattaccaccctgcattatccggcgccgaaacgcaacaaaccgaccgtgtatggcgtgagcccgaactattaa
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_J23106_sequence
1
tttacggctagctcagtcctaggtatagtgctagc
BBa_K2082291_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaa
BBa_K2082225_sequence
1
tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaaggattcagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagctgaacaaaagcttatttctgaagaggacttgagcctggaaaaacatagctggtatcatggcccggtgagccgcaacgcggcggaatatctgctgagcagcggcattaacggcagctttctggtgcgcgaaagcgaaagcagcccgggccagcgcagcattagcctgcgctatgaaggccgcgtgtatcattatcgcattaacaccgcgagcgatggcaaactgtatgtgagcagcgaaagccgctttaacaccctggcggaactggtgcatcatcatagcaccgtggcggatggcctgattaccaccctgcattatccggcgccgaaacgcaacaaaccgaccgtgtatggcgtgagcccgaactattaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z