LOCUS       BBa_K2082238            1779 bp    DNA     linear       01-Jan-2026
DEFINITION  Beta-lactamase under the control of a modified lacZ promoter
ACCESSION   BBa_K2082238
VERSION     BBa_K2082238.1  
FEATURES             Location/Qualifiers
     misc_feature    4..17
                     /label=OR1 binding site of cI (434)
     promoter        19..75
                     /label=optimized lacZ promotor
     promoter        1..75
                     /label=BBa_K2082210
     misc_feature    88..91
                     /label=conserved
     RBS             84..95
                     /label=BBa_B0034
     CDS             102..962
                     /label=BBa_K2082292
     misc_feature    971..1009
                     /label=B1006
     stem_loop       980..1001
                     /label=modified thr terminator
     polyA_site      1002..1009
                     /label=PolyA
     polyA_site      971..979
                     /label=PolyA
     terminator      971..1009
                     /label=BBa_B1006
     promoter        1018..1052
                     /label=BBa_J23106
     misc_feature    1065..1068
                     /label=conserved
     RBS             1061..1072
                     /label=BBa_B0034
     CDS             1079..1363
                     /label=434 cI
     misc_feature    1364..1393
                     /label=cMyc linker
     CDS             1394..1732
                     /label=SH2 domain of Abl1
     CDS             1079..1732
                     /label=BBa_K2082207
     misc_feature    1741..1779
                     /label=B1006
     stem_loop       1750..1771
                     /label=modified thr terminator
     polyA_site      1772..1779
                     /label=PolyA
     polyA_site      1741..1749
                     /label=PolyA
     terminator      1741..1779
                     /label=BBa_B1006
     misc_feature    1018..1779
                     /label=BBa_K2082225
ORIGIN
        1 aatacaagaa agtttgttca ttaggcaccc cgggctttac tcgtaaagct tccggcgcgt
       61 atgttgtgtc gaccgtacta gagaaagagg agaaatacta gatgagtatt caacatttcc
      121 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      181 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tatatcgaac
      241 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      301 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
      361 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
      421 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      481 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
      541 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
      601 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
      661 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
      721 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
      781 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
      841 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      901 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      961 aatactagag aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagttt
     1021 acggctagct cagtcctagg tatagtgcta gctactagag aaagaggaga aatactagat
     1081 gagtatttct tccagggtaa aaagcaaaag gattcagctt ggacttaacc aggctgaact
     1141 tgctcaaaag gtggggacta cccagcagtc tatagagcag ctcgaaaacg gtaaaactaa
     1201 gcgaccacgc tttttaccag aacttgcgtc agctcttggc gtaagtgttg actggctgct
     1261 caatggcacc tctgattcga atgttagatt tgttgggcac gttgagccca aagggaaata
     1321 tccattgatt agcatggtta gagctggttc gtggtgtgaa gctgaacaaa agcttatttc
     1381 tgaagaggac ttgagcctgg aaaaacatag ctggtatcat ggcccggtga gccgcaacgc
     1441 ggcggaatat ctgctgagca gcggcattaa cggcagcttt ctggtgcgcg aaagcgaaag
     1501 cagcccgggc cagcgcagca ttagcctgcg ctatgaaggc cgcgtgtatc attatcgcat
     1561 taacaccgcg agcgatggca aactgtatgt gagcagcgaa agccgcttta acaccctggc
     1621 ggaactggtg catcatcata gcaccgtggc ggatggcctg attaccaccc tgcattatcc
     1681 ggcgccgaaa cgcaacaaac cgaccgtgta tggcgtgagc ccgaactatt aatactagag
     1741 aaaaaaaaac cccgcccctg acagggcggg gtttttttt
//