LOCUS BBa_K2082251 3012 bp DNA linear 09-Jan-2025 DEFINITION Sequence for rpoZ Knock-Out in E. coli through CRISPR/Cas9 ACCESSION BBa_K2082251 VERSION BBa_K2082251.1 FEATURES Location/Qualifiers misc_feature 1..991 /label=Fragment A misc_feature 992..1066 /label=BBa_K2082210 misc_feature 1075..1086 /label=BBa_B0034 misc_feature 1093..1953 /label=BBa_K2082292 misc_feature 1962..2000 /label=BBa_B1006 misc_feature 2001..3012 /label=Fragment B ORIGIN 1 cacacagatg attgccagca caagatacct attaatatcg ccatccatac tttcatcatc 61 acctccgctg tagatagtca ggcagatata caacgtgatg aaagcaaagc cgagtggcaa 121 aaacggagtc tgcgaggacg cttcctgaaa atcgtcttta ttgcagtgaa tgacaggcaa 181 atgcggaagc agctacgcaa aacgcaacaa ctttgcgcaa aaagtgtgag caagggctac 241 gtcacatggc cgcgccgtgt ataataagct cgtatgtagg ctttatttcg ctaatcacat 301 acgaaagata ctcatggctc aaggcacgct ttatattgtt tctgccccca gtggcgcggg 361 taaatccagc ctgattcagg ctttattaaa aacccaaccg ttgtatgaca cccaggtttc 421 tgtttcacac accacacgcc aaccgcgtcc tggtgaagtc cacggtgaac attatttctt 481 tgttaatcat gatgaattta aagaaatgat tagcagagat gcgttcctcg aacacgcaga 541 agtttttggt aattactatg gcacttcgcg tgaggccatt gagcaagtac tggcgaccgg 601 tgtcgatgtt tttctcgata tcgactggca gggcgcgcag caaattcgcc agaagatgcc 661 gcacgcgcgg agtatcttta ttttaccgcc gtccaaaatt gaactggacc gccgtctacg 721 cggtcgcggt caggacagcg aagaggtcat tgcaaagcgt atggcgcaag ctgttgcaga 781 aatgagccat tacgccgaat atgattatct gattgtgaat gatgacttcg ataccgcgtt 841 gaccgatttg aagaccatta ttcgcgccga acgtctgcgc atgagccgcc aaaagcagcg 901 tcatgacgct ttaatcagca aattgttggc agactgaacc tgatttcagt atcatgccca 961 gtcatttctt cacctgtgga gctttttaag taatacaaga aagtttgttc attaggcacc 1021 ccgggcttta ctcgtaaagc ttccggcgcg tatgttgtgt cgaccgtact agagaaagag 1081 gagaaatact agatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 1141 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 1201 cagttgggtg cacgagtggg ttatatcgaa ctggatctca acagcggtaa gatccttgag 1261 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 1321 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 1381 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 1441 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 1501 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 1561 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 1621 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 1681 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 1741 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 1801 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 1861 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 1921 gagataggtg cctcactgat taagcattgg taatactaga gaaaaaaaaa ccccgcccct 1981 gacagggcgg ggtttttttt tcacaaagcg ggtcgccctt gtatctgttt gaaagcctga 2041 atcaactgat tcaaacctac ctgccggaag accaaatcaa gcgtctgcgg caggcgtatc 2101 tcgttgcacg tgatgctcac gaggggcaaa cacgttcaag cggtgaaccc tatatcacgc 2161 acccggtagc ggttgcctgc attctggccg agatgaaact cgactatgaa acgctgatgg 2221 cggcgctgct gcatgacgtg attgaagata ctcccgccac ctaccaggat atggaacagc 2281 tttttggtaa aagcgtcgcc gagctggtag agggggtgtc gaaacttgat aaactcaagt 2341 tccgcgataa gaaagaggcg caggccgaaa actttcgcaa gatgattatg gcgatggtgc 2401 aggatatccg cgtcatcctc atcaaacttg ccgaccgtac ccacaacatg cgcacgctgg 2461 gctcacttcg cccggacaaa cgtcgccgca tcgcccgtga aactctcgaa atttatagcc 2521 cgctggcgca ccgtttaggt atccaccaca ttaaaaccga actcgaagag ctgggttttg 2581 aggcgctgta tcccaaccgt tatcgcgtaa tcaaagaagt ggtgaaagcc gcgcgcggca 2641 accgtaaaga gatgatccag aagattcttt ctgaaatcga agggcgtttg caggaagcgg 2701 gaataccgtg ccgcgtcagt ggtcgcgaga agcatcttta ttcgatttac tgcaaaatgg 2761 tgctcaaaga gcagcgtttt cactcgatca tggacatcta cgctttccgc gtgatcgtca 2821 atgattctga cacctgttat cgcgtgctgg gccagatgca cagcctgtac aagccgcgtc 2881 cgggccgcgt gaaagactat atcgccattc caaaagcgaa cggctatcag tctttgcaca 2941 cctcgatgat cggcccgcac ggtgtgccgg ttgaggtcca gatccgtacc gaagatatgg 3001 accagatggc gg //