LOCUS       BBa_K2082252            2024 bp    DNA     linear       29-Jun-2024
DEFINITION  RFP under the control of an optimized lacZ promoter with lambda cI 
            binding site combined with SH2:cI
ACCESSION   BBa_K2082252
VERSION     BBa_K2082252.1  
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label=OR1 of phage lambda
     promoter        28..84
                     /label=modified lacZ promoter
     promoter        1..84
                     /label=BBa_K2082299
     misc_feature    97..100
                     /label=conserved
     RBS             93..104
                     /label=BBa_B0034
     CDS             111..788
                     /label=RFP
     CDS             111..788
                     /label=BBa_K2082291
     misc_feature    797..835
                     /label=B1006
     stem_loop       806..827
                     /label=modified thr terminator
     polyA_site      828..835
                     /label=PolyA
     polyA_site      797..805
                     /label=PolyA
     terminator      797..835
                     /label=BBa_B1006
     promoter        844..878
                     /label=BBa_J23106
     misc_feature    891..894
                     /label=conserved
     RBS             887..898
                     /label=BBa_B0034
     CDS             907..1611
                     /label=cI of phage lambda
     misc_feature    1612..1641
                     /label=cMyc-linker
     CDS             1642..1977
                     /label=SH2 domain
     CDS             907..1977
                     /label=BBa_K2082300
     misc_feature    1986..2024
                     /label=B1006
     stem_loop       1995..2016
                     /label=modified thr terminator
     polyA_site      2017..2024
                     /label=PolyA
     polyA_site      1986..1994
                     /label=PolyA
     terminator      1986..2024
                     /label=BBa_B1006
ORIGIN
        1 tttactatca ccgccagagg taacgtacat taggcacccc gggctttact cgtaaagctt
       61 ccggcgcgta tgttgtgtcg accgtactag agaaagagga gaaatactag atggcttcct
      121 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
      181 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
      241 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
      301 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
      361 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
      421 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
      481 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
      541 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
      601 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
      661 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
      721 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
      781 gtgcttaata ctagagaaaa aaaaaccccg cccctgacag ggcggggttt ttttttacta
      841 gagtttacgg ctagctcagt cctaggtata gtgctagcta ctagagaaag aggagaaata
      901 ctagagagca caaaaaagaa accattaaca caagagcagc ttgaggacgc acgtcgcctt
      961 aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac
     1021 aagatgggga tggggcagtc aggcgttggt gctttattta atggcatcaa tgcattaaat
     1081 gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct
     1141 tcaatcgcca gagaaatcta cgagatgtat gaagcggtta gtatgcagcc gtcacttaga
     1201 agtgagtatg agtaccctgt tttttctcat gttcaggcag ggatgttctc acctgagctt
     1261 agaaccttta ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa agccagtgat
     1321 tctgcattct ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca
     1381 agctttcctg acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat
     1441 ttctgcatag ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc
     1501 ggtcaggtgt ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt
     1561 tgttccgttg tggggaaagt tatcgctagt cagtggcctg aagagacgtt tgaacaaaag
     1621 cttatttctg aagaggactt gagcctggaa aaacatagct ggtatcatgg cccggtgagc
     1681 cgcaacgcgg cggaatatct gctgagcagc ggcattaacg gcagctttct ggtgcgcgaa
     1741 agcgaaagca gcccgggcca gcgcagcatt agcctgcgct atgaaggccg cgtgtatcat
     1801 tatcgcatta acaccgcgag cgatggcaaa ctgtatgtga gcagcgaaag ccgctttaac
     1861 accctggcgg aactggtgca tcatcatagc accgtggcgg atggcctgat taccaccctg
     1921 cattatccgg cgccgaaacg caacaaaccg accgtgtatg gcgtgagccc gaactattac
     1981 tagagaaaaa aaaaccccgc ccctgacagg gcggggtttt tttt
//