BBa_K2082300 1 BBa_K2082300 SH2 domain fused with cI of phage lambda 2016-10-13T11:00:00Z 2016-10-14T08:09:56Z Coming soon Coming soon false false _2550_ 30782 30782 9 false Coming soon false Pascal Schmidt annotation2505733 1 cI of phage lambda range2505733 1 1 705 annotation2505781 1 SH2 domain range2505781 1 736 1071 annotation2505734 1 cMyc-linker range2505734 1 706 735 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K2082299 1 BBa_K2082299 Modified lacZ promoter with OR1 binding site for cI of lambda 2016-10-13T11:00:00Z 2016-10-14T08:03:23Z Coming soon Coming soon false false _2550_ 30782 30782 9 false Coming soon false Pascal Schmidt annotation2505700 1 OR1 of phage lambda range2505700 1 1 26 annotation2505703 1 modified lacZ promoter range2505703 1 28 84 BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 annotation1898431 1 PolyA range1898431 1 1 9 annotation1898429 1 modified thr terminator range1898429 1 10 31 BBa_K2082252 1 BBa_K2082252 RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI 2016-10-13T11:00:00Z 2016-10-21T07:27:19Z Coming soon Coming soon false false _2550_ 30782 30782 9 false Coming soon false Pascal Schmidt component2505844 1 BBa_K2082299 component2505848 1 BBa_K2082291 component2505860 1 BBa_K2082300 component2505846 1 BBa_B0034 component2505854 1 BBa_J23106 component2505865 1 BBa_B1006 component2505856 1 BBa_B0034 component2505853 1 BBa_B1006 annotation2505848 1 BBa_K2082291 range2505848 1 111 788 annotation2505853 1 BBa_B1006 range2505853 1 797 835 annotation2505854 1 BBa_J23106 range2505854 1 844 878 annotation2505865 1 BBa_B1006 range2505865 1 1986 2024 annotation2505844 1 BBa_K2082299 range2505844 1 1 84 annotation2505856 1 BBa_B0034 range2505856 1 887 898 annotation2505860 1 BBa_K2082300 range2505860 1 907 1977 annotation2505846 1 BBa_B0034 range2505846 1 93 104 BBa_K2082291 1 BBa_K2082291 Red fluorescence protein 2016-10-13T11:00:00Z 2016-10-14T07:00:45Z Coming soon Coming soon false false _2550_ 30782 30782 9 false Coming soon false Pascal Schmidt annotation2503254 1 RFP range2503254 1 1 678 BBa_J23106 1 BBa_J23106 constitutive promoter family member 2006-08-13T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034_sequence 1 aaagaggagaaa BBa_K2082252_sequence 1 tttactatcaccgccagaggtaacgtacattaggcaccccgggctttactcgtaaagcttccggcgcgtatgttgtgtcgaccgtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagtttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagagagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgaacaaaagcttatttctgaagaggacttgagcctggaaaaacatagctggtatcatggcccggtgagccgcaacgcggcggaatatctgctgagcagcggcattaacggcagctttctggtgcgcgaaagcgaaagcagcccgggccagcgcagcattagcctgcgctatgaaggccgcgtgtatcattatcgcattaacaccgcgagcgatggcaaactgtatgtgagcagcgaaagccgctttaacaccctggcggaactggtgcatcatcatagcaccgtggcggatggcctgattaccaccctgcattatccggcgccgaaacgcaacaaaccgaccgtgtatggcgtgagcccgaactattactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_K2082299_sequence 1 tttactatcaccgccagaggtaacgtacattaggcaccccgggctttactcgtaaagcttccggcgcgtatgttgtgtcgaccg BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_J23106_sequence 1 tttacggctagctcagtcctaggtatagtgctagc BBa_K2082300_sequence 1 agcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgaacaaaagcttatttctgaagaggacttgagcctggaaaaacatagctggtatcatggcccggtgagccgcaacgcggcggaatatctgctgagcagcggcattaacggcagctttctggtgcgcgaaagcgaaagcagcccgggccagcgcagcattagcctgcgctatgaaggccgcgtgtatcattatcgcattaacaccgcgagcgatggcaaactgtatgtgagcagcgaaagccgctttaacaccctggcggaactggtgcatcatcatagcaccgtggcggatggcctgattaccaccctgcattatccggcgccgaaacgcaacaaaccgaccgtgtatggcgtgagcccgaactat BBa_K2082291_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z