BBa_K2083001 1 BBa_K2083001 APOBEC 2XTEN 2016-10-13T11:00:00Z 2016-10-14T10:10:45Z This part comes from the sequence of the enzyme in rats. Coding region for APOBEC1, an enzyme that performs C to U edits on DNA and RNA with a 2xXTEN (88 nucleotide) linker. The one extension linker sequence is TCTGGCTCAGAAACG-CCCGGTACGTCCGAGTCTGCAACACCAGAAAGC. The 2xXTEN is the one extension sequence twice. APOBEC with a 1xXTEN is BBa_K2083000, and the 3xXTEN can be found here BBa_K2083002. Source: Komor AC et al.Nature. Epub ahead of print (2016). false false _2551_ 31287 31287 9 false We preformed codon optimization to get rid of any EcoRI, XbaI, SpeI, and PstI sites. false Virginia Massa BBa_K2083001_sequence 1 atggactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagtcttctgaaaccggcccggtcgctgtagaccccaccctccgccgaaggattgagccacacgaatttgaggtgttttttgacccccgggagctgcgcaaggaaacttgcctcctctacgagattaattggggcggacggcattctatttggcgccacacgtcccaaaatacaaataagcacgttgaggtgaatttcattgaaaagttcacgacggagcggtacttttgcccaaatactcggtgcagtattacatggtttcttagttggtcaccatgcggtgaatgttcccgcgctataaccgagttcctcagtaggtacccccatgtcacgctgtttatctatatcgcacgactttaccatcacgccgaccctcggaaccggcagggactccgggatcttatttcatcaggagttaccatccaaattatgaccgaacaggagtcaggttattgctggcggaactttgtgaattactctccaagcaatgaggcacattggccacgctatccccatctgtgggtcaggctttatgtcttggagctctactgtataatcttggggcttccgccgtgcctcaacattcttcgaaggaagcagccacagctcacctttttcaccatcgccctgcaatcctgccactaccagcgactgccaccgcacattctttgggccaccggacttaagtctggctcagaaacgcccggtacgtccgagtctgcaacaccagaaagctccggatctgagactccaggaacttccgaatctgctactccagagagctcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z