BBa_K2083012 1 BBa_K2083012 Wild Type Human Beta Globin Fused to GFP Reporter 2016-10-13T11:00:00Z 2016-10-14T03:54:16Z This a human beta globin sequence with the first two exons fused. GFP is fused to the end of the end of the sequence. Coding region for human beta globin with GFP reporter fused to the end. GFP fused to beta globin with a premature termination codon (PTC) can be found at BBa K2083011. The validation of the β-globin reporters followed the same procedures as the ATG/ACG reporters. The wild type β-globin reporter showed a much lower level of expression compared to the ATG reporter (14.77% of positive control). http://2016.igem.org/wiki/images/8/85/T--WPI_Worcester--globineGFP_data_.png Figure 1: Efficiency of WT/PTC β-globin reporters compared to positive control. Error bars represent standard error between normalized results from 3 biological replicates of the experiment. false false _2551_ 0 31287 31287 9 Not in stock false We performed codon optimization to removed any illegal restriction sites. false Virginia Massa BBa_K2083012_sequence 1 atggtgcatctgtccagtgaggagaagtctgcggtcactgccctgtggggcaaggtgaatgtggaagaagttggtggtgaggccctgggcaggctgctggttgtctacccatggacccagaggttcttcgagtcctttggggacctgtcctctgcaaatgctgttatgaacaatcctaaggtgaaggctcatggcaagaaggtgctggctgccttcagtgagggtctgagtcacctggacaacctcaaaggcacctttgctcagctgagtgaactgcactgtgacaagctgcacgtcgatcctgagaacttcagggtgagtttggggacccttgattgttctttctttttcgctattgtaaaattcatgttatatggagggggcaaagttttcagggtgttgtttagaatgggaagatgtcccttgtatcaccatggaccctcatgataattttgtttctttcactttctactctgttgacaaccattgtctcctcttattttcttttcattttctgtaactttttcgttaaactttagcttgcatttgtaacgaatttttaaattcacttttgtttatttgtcagattgtaagtactttctctaatcacttttttttcaaggcaatcagggtatattatattgtacttcagcacagttttagagaacaattgttataattaaatgataaggtagaatatttctgcatataaattctggctggcgtggaaatattcttattggtagaaacaactacaccctggtcatcatcctgcctttctctttatggttacaatgatatacactgtttgagatgaggataaaatactctgagtccaaaccgggcccctctgctaaccatgttcatgccttcttctctttcctacagctcctgggcaacgtgctggttgttgtgctgtctcatcattttggcaaagagttcactcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z