LOCUS       BBa_K2084005            3203 bp    DNA     linear       14-May-2024
DEFINITION  pT7 Toehold + lacz reporter
ACCESSION   BBa_K2084005
VERSION     BBa_K2084005.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=BBa_K2084002
     misc_feature    3152..3152
                     /label=C->T
     CDS             129..3203
                     /label=lacZ
     misc_feature    129..131
                     /label=START
     misc_feature    3201..3203
                     /label=STOP
     CDS             129..3203
                     /label=BBa_I732005
ORIGIN
        1 taatacgact cactataggg agagggatct attactactt accattgtct tgctctatac
       61 agaaacagag gagatataga atgagacaat ggaacctggc ggcagcgcaa aagatgcgta
      121 aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg
      181 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      241 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      301 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
      361 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
      421 cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg
      481 agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag
      541 gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc
      601 gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt
      661 tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc
      721 tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata
      781 aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc
      841 gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa
      901 cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg
      961 aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa
     1021 acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca
     1081 ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga
     1141 ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc
     1201 acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc
     1261 tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc
     1321 tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa
     1381 cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga
     1441 gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt
     1501 cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat
     1561 ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg
     1621 atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga
     1681 aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg
     1741 aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc
     1801 gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta
     1861 aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga
     1921 acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc
     1981 tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg
     2041 aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg
     2101 cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta
     2161 aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca
     2221 cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct
     2281 ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca
     2341 tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc
     2401 aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc
     2461 tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg
     2521 aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc
     2581 aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta
     2641 cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc
     2701 ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac
     2761 cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact
     2821 ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc
     2881 gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc
     2941 tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt
     3001 tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc
     3061 acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg
     3121 actcctggag cccgtcagta tcggcggaat ttcagctgag cgccggtcgc taccattacc
     3181 agttggtctg gtgtcaaaaa taa
//