LOCUS       BBa_K2084007            2830 bp    DNA     linear       26-Dec-2024
DEFINITION  pT7 controlled T3 RNAP
ACCESSION   BBa_K2084007
VERSION     BBa_K2084007.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..2693
                     /label=BBa_K2084001
     stem_loop       2713..2756
                     /label=stem_loop
     misc_feature    2702..2781
                     /label=BBa_B0010
     terminator      2702..2781
                     /label=BBa_B0010
     stem_loop       2797..2816
                     /label=T7 TE
     misc_feature    2823..2823
                     /label=stop
     polyA_site      2817..2830
                     /label=polya
     misc_feature    2790..2830
                     /label=BBa_B0012
     terminator      2790..2830
                     /label=BBa_B0012
     terminator      2702..2830
                     /label=BBa_B0015
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaacatcatc gaaaacatcg
       61 aaaagaatga cttctcagaa atcgaactgg ctgctatccc gttcaacaca ctggctgacc
      121 actacggaag cgccttggct aaagagcagt tggctttaga acatgagtct tatgagctag
      181 gcgagcgccg cttcctcaag atgcttgagc gtcaagcgaa agctggtgag attgcagaca
      241 acgcagccgc taagccgtta ctcgctacgc ttctccctaa gttaaccaca cgtatcgtcg
      301 agtggctcga agagtacgca tcgaagaaag gccgcaagcc tagcgcatac gcaccgctcc
      361 agttactcaa gccggaggcc tccgcgttta tcaccctgaa agttatcctt gcgtcactaa
      421 ccagtacgaa catgacaacc attcaggccg ctgctggtat gctggggaaa gccattgagg
      481 acgaggcacg atttgggcgc atccgtgacc tagaagcgaa gcacttcaag aagcacgttg
      541 aggaacagct taacaagcgc cacgggcaag tctacaagaa agcatttatg caggtggtcg
      601 aggccgatat gattggtcga ggtctgcttg gtggcgaggc gtggtctagc tgggataaag
      661 aaaccacgat gcacgtaggg attcgcctga ttgaaatgct gattgaatcc acgggtctgg
      721 tggaattaca gcgccacaac gcaggtaacg caggctctga ccatgaggca ctgcaactgg
      781 cccaagagta cgtggacgta ttagcgaagc gtgcaggcgc tctggcgggt atctctccga
      841 tgttccagcc gtgtgtcgta ccgccgaaac cttgggtagc aatcacaggg ggcggctatt
      901 gggctaacgg tcgcagacct ttggcactcg ttcgcactca ctctaagaag ggcttgatgc
      961 gctacgaaga cgtttacatg ccagaagtct acaaggctgt gaacctcgcg caaaacaccg
     1021 catggaaaat caacaagaaa gttcttgctg ttgtcaatga gattgttaac tggaagaatt
     1081 gcccggtagc agacattcca tcgctggagc gccaagagtt accgcctaag cctgacgaca
     1141 ttgacaccaa cgaggcagcg ctcaaggagt ggaagaaagc cgctgctggt atctatcgct
     1201 tggacaaggc acgagtgtct cgccgtatca gcttagagtt catgctggag caggccaaca
     1261 agttcgcaag taagaaagca atctggttcc cttacaacat ggactggcgc ggtcgtgtgt
     1321 acgctgtgcc gatgttcaac ccgcaaggca acgacatgac gaaaggtctg ctgacccttg
     1381 ctaaaggcaa gccaatcggt gaggaaggtt tctactggct gaaaatccac ggtgcgaact
     1441 gtgcgggtgt tgataaggtt ccattcccgg agcgcatcgc gttcattgag aagcacgtag
     1501 acgacattct ggcttgcgct aaagacccaa tcaataacac ttggtgggct gagcaggatt
     1561 caccgttctg tttcctcgcg ttttgcttcg agtatgcagg cgttacgcac cacggtctga
     1621 gctacaattg ctctctgccg ctggcgttcg acgggtcttg ctctggtatc cagcacttct
     1681 ccgcgatgct ccgcgatgag gtaggcggtc gtgcggttaa cctgctgcca agcgaaaccg
     1741 tgcaggacat ttacggcatc gttgcacaga aagtaaacga gattctcaaa caggatgcaa
     1801 tcaacggcac gcctaacgag atgattaccg tgaccgacaa ggacaccggg gaaatctcag
     1861 agaagctcaa acttggaacc tcaacgctgg cgcaacagtg gctggcatat ggtgtaaccc
     1921 gtagcgtaac taaacgttcg gtcatgacgc tggcttacgg ttccaaggag ttcggctttc
     1981 gtcaacaggt attggatgac accattcagc ctgcaattga cagcggtaag ggcttgatgt
     2041 tcacccaacc gaaccaagcg gctggctata tggctaagct gatttgggat gcggtaagcg
     2101 tgaccgtagt tgcagcggtt gaggcgatga actggctcaa atctgccgct aagctgctgg
     2161 ctgctgaggt caaggacaag aagaccaagg agattctgcg ccaccgttgc gcggttcact
     2221 ggactacgcc ggacggcttc ccggtctggc aggaataccg caagccactc cagaagcgtc
     2281 tcgatatgat tttcttaggg caattccgtc tgcaaccgac gattaatacc ctcaaggatt
     2341 caggcattga cgcacacaag caggagtctg gcatcgctcc taactttgtt cactcacagg
     2401 acggtagcca cctccgcatg acagtcgttt atgctcacga gaagtatggc attgagtcct
     2461 ttgcgctcat ccatgacagc tttgggacta tcccggcaga cgctggtaag ctctttaagg
     2521 ctgtgcgtga aacgatggtt atcacctatg agaacaacga tgtgctggca gacttctact
     2581 ctcagtttgc cgaccagcta cacgagaccc aactggacaa gatgcctccg cttccgaaga
     2641 aaggaaacct gaacctgcaa gacattctca agtctgactt tgcctttgca taatactaga
     2701 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2761 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2821 tgcgtttata
//