LOCUS       BBa_K2084008            2821 bp    DNA     linear       22-Nov-2024
DEFINITION  pT3 Amplifier
ACCESSION   BBa_K2084008
VERSION     BBa_K2084008.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_K2084000
     misc_feature    30..2684
                     /label=BBa_K2084001
     stem_loop       2704..2747
                     /label=stem_loop
     misc_feature    2693..2772
                     /label=BBa_B0010
     terminator      2693..2772
                     /label=BBa_B0010
     stem_loop       2788..2807
                     /label=T7 TE
     misc_feature    2814..2814
                     /label=stop
     polyA_site      2808..2821
                     /label=polya
     misc_feature    2781..2821
                     /label=BBa_B0012
     terminator      2781..2821
                     /label=BBa_B0012
ORIGIN
        1 aattaaccct cactaaaggg agatactaga tgaacatcat cgaaaacatc gaaaagaatg
       61 acttctcaga aatcgaactg gctgctatcc cgttcaacac actggctgac cactacggaa
      121 gcgccttggc taaagagcag ttggctttag aacatgagtc ttatgagcta ggcgagcgcc
      181 gcttcctcaa gatgcttgag cgtcaagcga aagctggtga gattgcagac aacgcagccg
      241 ctaagccgtt actcgctacg cttctcccta agttaaccac acgtatcgtc gagtggctcg
      301 aagagtacgc atcgaagaaa ggccgcaagc ctagcgcata cgcaccgctc cagttactca
      361 agccggaggc ctccgcgttt atcaccctga aagttatcct tgcgtcacta accagtacga
      421 acatgacaac cattcaggcc gctgctggta tgctggggaa agccattgag gacgaggcac
      481 gatttgggcg catccgtgac ctagaagcga agcacttcaa gaagcacgtt gaggaacagc
      541 ttaacaagcg ccacgggcaa gtctacaaga aagcatttat gcaggtggtc gaggccgata
      601 tgattggtcg aggtctgctt ggtggcgagg cgtggtctag ctgggataaa gaaaccacga
      661 tgcacgtagg gattcgcctg attgaaatgc tgattgaatc cacgggtctg gtggaattac
      721 agcgccacaa cgcaggtaac gcaggctctg accatgaggc actgcaactg gcccaagagt
      781 acgtggacgt attagcgaag cgtgcaggcg ctctggcggg tatctctccg atgttccagc
      841 cgtgtgtcgt accgccgaaa ccttgggtag caatcacagg gggcggctat tgggctaacg
      901 gtcgcagacc tttggcactc gttcgcactc actctaagaa gggcttgatg cgctacgaag
      961 acgtttacat gccagaagtc tacaaggctg tgaacctcgc gcaaaacacc gcatggaaaa
     1021 tcaacaagaa agttcttgct gttgtcaatg agattgttaa ctggaagaat tgcccggtag
     1081 cagacattcc atcgctggag cgccaagagt taccgcctaa gcctgacgac attgacacca
     1141 acgaggcagc gctcaaggag tggaagaaag ccgctgctgg tatctatcgc ttggacaagg
     1201 cacgagtgtc tcgccgtatc agcttagagt tcatgctgga gcaggccaac aagttcgcaa
     1261 gtaagaaagc aatctggttc ccttacaaca tggactggcg cggtcgtgtg tacgctgtgc
     1321 cgatgttcaa cccgcaaggc aacgacatga cgaaaggtct gctgaccctt gctaaaggca
     1381 agccaatcgg tgaggaaggt ttctactggc tgaaaatcca cggtgcgaac tgtgcgggtg
     1441 ttgataaggt tccattcccg gagcgcatcg cgttcattga gaagcacgta gacgacattc
     1501 tggcttgcgc taaagaccca atcaataaca cttggtgggc tgagcaggat tcaccgttct
     1561 gtttcctcgc gttttgcttc gagtatgcag gcgttacgca ccacggtctg agctacaatt
     1621 gctctctgcc gctggcgttc gacgggtctt gctctggtat ccagcacttc tccgcgatgc
     1681 tccgcgatga ggtaggcggt cgtgcggtta acctgctgcc aagcgaaacc gtgcaggaca
     1741 tttacggcat cgttgcacag aaagtaaacg agattctcaa acaggatgca atcaacggca
     1801 cgcctaacga gatgattacc gtgaccgaca aggacaccgg ggaaatctca gagaagctca
     1861 aacttggaac ctcaacgctg gcgcaacagt ggctggcata tggtgtaacc cgtagcgtaa
     1921 ctaaacgttc ggtcatgacg ctggcttacg gttccaagga gttcggcttt cgtcaacagg
     1981 tattggatga caccattcag cctgcaattg acagcggtaa gggcttgatg ttcacccaac
     2041 cgaaccaagc ggctggctat atggctaagc tgatttggga tgcggtaagc gtgaccgtag
     2101 ttgcagcggt tgaggcgatg aactggctca aatctgccgc taagctgctg gctgctgagg
     2161 tcaaggacaa gaagaccaag gagattctgc gccaccgttg cgcggttcac tggactacgc
     2221 cggacggctt cccggtctgg caggaatacc gcaagccact ccagaagcgt ctcgatatga
     2281 ttttcttagg gcaattccgt ctgcaaccga cgattaatac cctcaaggat tcaggcattg
     2341 acgcacacaa gcaggagtct ggcatcgctc ctaactttgt tcactcacag gacggtagcc
     2401 acctccgcat gacagtcgtt tatgctcacg agaagtatgg cattgagtcc tttgcgctca
     2461 tccatgacag ctttgggact atcccggcag acgctggtaa gctctttaag gctgtgcgtg
     2521 aaacgatggt tatcacctat gagaacaacg atgtgctggc agacttctac tctcagtttg
     2581 ccgaccagct acacgagacc caactggaca agatgcctcc gcttccgaag aaaggaaacc
     2641 tgaacctgca agacattctc aagtctgact ttgcctttgc ataatactag agccaggcat
     2701 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2761 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2821 a
//