LOCUS       BBa_K2086002            1499 bp    DNA     linear       01-Jun-2024
DEFINITION  PyeaR - SerA
ACCESSION   BBa_K2086002
VERSION     BBa_K2086002.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     RBS             109..123
                     /label=RBS-1\Strong
     RBS             116..120
                     /label=RBS
     misc_feature    109..123
                     /label=BBa_B0030
     RBS             109..123
                     /label=BBa_B0030
     CDS             130..1362
                     /label=BBa_K2086001
     stem_loop       1382..1425
                     /label=stem_loop
     misc_feature    1371..1450
                     /label=BBa_B0010
     terminator      1371..1450
                     /label=BBa_B0010
     stem_loop       1466..1485
                     /label=T7 TE
     misc_feature    1492..1492
                     /label=stop
     polyA_site      1486..1499
                     /label=polya
     misc_feature    1459..1499
                     /label=BBa_B0012
     terminator      1459..1499
                     /label=BBa_B0012
     terminator      1371..1499
                     /label=BBa_B0015
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagat taaagaggag
      121 aaatactaga tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa
      181 ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt
      241 cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc
      301 ggcctgcgat cccgtaccca tctgactgaa gacgtgatca acgccgcaga aaaactggtc
      361 gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc
      421 gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga gctggtgatt
      481 ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcaccgtggc
      541 gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc
      601 ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt
      661 tacttttatg atattgaaaa taaactgccg ctgggcaacg ccactcaggt acagcatctt
      721 tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtccacc
      781 aaaaatatga tgggcgcgaa agaaatttca ctaatgaagc ccggctcgct gctgattaat
      841 gcttcgcgcg gtactgtggt ggatattccg gcgctgtgtg atgcgctggc gagcaaacat
      901 ctggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag cgatccattt
      961 acctctccgc tgtgtgaatt tgacaacgtc cttctgacgc cacacattgg cggttcgact
     1021 caggaagcgc aggagaatat cggcctggaa gttgcgggta aattgatcaa gtattctgac
     1081 aatggctcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcacggtggg
     1141 cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc taactgcgct gaacaaaatc
     1201 ttcgccgagc agggcgtcaa catcgccgcg caatatctgc aaacttccgc ccagatgggt
     1261 tatgtggtta ttgatattga agccgacgaa gacgttgccg aaaaagcgct gcaagcaatg
     1321 aaagctattc cgggtaccat tcgcgcccgt ctgctgtact aatactagag ccaggcatca
     1381 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1441 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//