##gff-version 3
##sequence-region BBa_K2086002 1 1499
BBa_K2086002	.	ribosome_entry_site	109	123	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K2086002	.	engineered_region	109	123	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2086002	.	ribosome_entry_site	116	120	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2086002	.	ribosome_entry_site	109	123	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2086002	.	CDS	130	1362	.	+	0	ID=BBa_K2086001;Name=BBa_K2086001
BBa_K2086002	.	terminator	1371	1499	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2086002	.	terminator	1459	1499	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2086002	.	stem_loop	1466	1485	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2086002	.	polyA_site	1486	1499	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2086002	.	stop_codon	1492	1492	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2086002	.	engineered_region	1459	1499	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2086002	.	terminator	1371	1450	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2086002	.	stem_loop	1382	1425	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2086002	.	engineered_region	1371	1450	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2086002	.	promoter	1	100	.	+	0	ID=BBa_K216005;Name=BBa_K216005
BBa_K2086002	.	non_covalent_binding_site	50	65	.	+	0	ID=annotation2027072;Name=NarL-binding region;Parent=BBa_K216005
BBa_K2086002	.	non_covalent_binding_site	58	80	.	+	0	ID=annotation2027073;Name=NsrR-binding region;Parent=BBa_K216005
BBa_K2086002	.	non_covalent_binding_site	66	71	.	+	0	ID=annotation2027074;Name=promoter -35 region;Parent=BBa_K216005
BBa_K2086002	.	non_covalent_binding_site	89	94	.	+	0	ID=annotation2027075;Name=promoter -10 region;Parent=BBa_K216005
>BBa_K2086002
ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaat
tatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatggcaaaggtatcgctggagaaagacaa
gattaagtttctgctggtagaaggcgtgcaccaaaaggcgctggaaagccttcgtgcagctggttacaccaacatcgaa
tttcacaaaggcgcgctggatgatgaacaattaaaagaatccatccgcgatgcccacttcatcggcctgcgatcccgta
cccatctgactgaagacgtgatcaacgccgcagaaaaactggtcgctattggctgtttctgtatcggaacaaaccaggt
tgatctggatgcggcggcaaagcgcgggatcccggtatttaacgcaccgttctcaaatacgcgctctgttgcggagctg
gtgattggcgaactgctgctgctattgcgcggcgtgccggaagccaatgctaaagcgcaccgtggcgtgtggaacaaac
tggcggcgggttcttttgaagcgcgcggcaaaaagctgggtatcatcggctacggtcatattggtacgcaattgggcat
tctggctgaatcgctgggaatgtatgtttacttttatgatattgaaaataaactgccgctgggcaacgccactcaggta
cagcatctttctgacctgctgaatatgagcgatgtggtgagtctgcatgtaccagagaatccgtccaccaaaaatatga
tgggcgcgaaagaaatttcactaatgaagcccggctcgctgctgattaatgcttcgcgcggtactgtggtggatattcc
ggcgctgtgtgatgcgctggcgagcaaacatctggcgggggcggcaatcgacgtattcccgacggaaccggcgaccaat
agcgatccatttacctctccgctgtgtgaatttgacaacgtccttctgacgccacacattggcggttcgactcaggaag
cgcaggagaatatcggcctggaagttgcgggtaaattgatcaagtattctgacaatggctcaacgctctctgcggtgaa
cttcccggaagtctcgctgccactgcacggtgggcgtcgtctgatgcacatccacgaaaaccgtccgggcgtgctaact
gcgctgaacaaaatcttcgccgagcagggcgtcaacatcgccgcgcaatatctgcaaacttccgcccagatgggttatg
tggttattgatattgaagccgacgaagacgttgccgaaaaagcgctgcaagcaatgaaagctattccgggtaccattcg
cgcccgtctgctgtactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata