BBa_K2088017 1 BBa_K2088017 J23100+B0034+BBa_K2088005 2016-10-08T11:00:00Z 2016-10-19T12:48:04Z This part is an optimization of BBa_K2088670. This part includes optimized mhbH (using Codon Optimization Tool of IDT) and a designed rbs (using RBS Calculator). mhbH encodes Fumarylpyruvate hydrolase. false false _2556_ 29893 33751 9 false An optimization of BBa_K2088670 with a designed rbs. false Xia Chen component2522020 1 BBa_B0034 component2522018 1 BBa_J23100 component2522025 1 BBa_K2088005 annotation2522025 1 BBa_K2088005 range2522025 1 62 1425 annotation2522020 1 BBa_B0034 range2522020 1 44 55 annotation2522018 1 BBa_J23100 range2522018 1 1 35 BBa_K2088005 1 BBa_K2088005 pbaC with double Terminator 2016-10-08T11:00:00Z 2016-10-19T12:17:49Z This part is an optimization of BBa_K2088667. This part includes optimized mhbD using Codon Optimization Tool of iDT and a designed rbs using RBS Calculator. false false _2556_ 33751 33751 9 false An optimization of BBa_K2088667 with a designed rbs. false Xia Chen annotation2521812 1 BBa_B0015 range2521812 1 1236 1364 annotation2521811 1 TAG Stop Codon range2521811 1 1225 1227 annotation2521810 1 ATG Start Codon range2521810 1 1 3 annotation2521813 1 pbaC coding sequence range2521813 1 1 1227 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_K2088017_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgagctattatgacgttctgatcgtgggggccggccatgccggggcgcaagcagcgatttccctgcgccagcttgggttcgaaggatcggtcgcaatgatcggcgatgaaaaagacccgccctatgaacgtcccccgctgtccaaggaatatttcgcgggcgacaagaacttcgatcgcatcctcatcaggcccgccgccttctggggggaacggaaggtcgacatgctgctgggcaagcgcgtgaaacatgtcgatccgaccgccaaattcgtgacggcgggggatgaggaaatcggctatggttcgctgatctgggcgacgggcggcagtccgcgcatgttgacctgcaacggtgcggatgcgtccaacgtccatgcggtccgccgccgcgacgatgtcgacgccatgatggcgaagctggaccagatcgaccatgtgaccgtgatcggcggcggctatatcgggctggaggccgccgctgtcctcaccaagttcggcaagaaggtggtgctgctggaggcgctcgaccgggtactcgcccgcgtggcgggggaagatctttcgcgcttttacgaggccgagcatcgcgcccatggcgttgacctgcggaccggaacgaagatggactgcatcgaggtcagggacggcaaggcgaccgcggtgctgatgcaggacggcgagcggatcgagacagacatggttatcgtcggcatcggcatcgtgccggaaaccggccccctgatcgcggcgggcgccgctggcggcaatggtgtcgatgtggacgaatattgccgcaccagcctgccggatatctatgccgtaggcgattgcgcggcgcatgccaaccgctttgcccggggcgcgcaaattcgcctcgaatccgtgcagaacgccaatgatcaggcgaagacggcggtggcgcacatcatgggcaaggaagacccctatgacgcggtgccgtggttctggtccaatcaatatgacctgaaattgcagacggtcgggctgtcgaccggccatgaccagacgctattgcggggcgatccggcggcgcgcagcttttccgtgctatatttgaagggcgggaagctgatcgcgctagactgcgtcaatgcggtgaaggactatgtgcaggggcgcgcccatgtgctgtccggcgcggcgctggatcaggcgcaactggccgatccgtccgtgccgctcaaggaagtggggctggcttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2088005_sequence 1 atgagctattatgacgttctgatcgtgggggccggccatgccggggcgcaagcagcgatttccctgcgccagcttgggttcgaaggatcggtcgcaatgatcggcgatgaaaaagacccgccctatgaacgtcccccgctgtccaaggaatatttcgcgggcgacaagaacttcgatcgcatcctcatcaggcccgccgccttctggggggaacggaaggtcgacatgctgctgggcaagcgcgtgaaacatgtcgatccgaccgccaaattcgtgacggcgggggatgaggaaatcggctatggttcgctgatctgggcgacgggcggcagtccgcgcatgttgacctgcaacggtgcggatgcgtccaacgtccatgcggtccgccgccgcgacgatgtcgacgccatgatggcgaagctggaccagatcgaccatgtgaccgtgatcggcggcggctatatcgggctggaggccgccgctgtcctcaccaagttcggcaagaaggtggtgctgctggaggcgctcgaccgggtactcgcccgcgtggcgggggaagatctttcgcgcttttacgaggccgagcatcgcgcccatggcgttgacctgcggaccggaacgaagatggactgcatcgaggtcagggacggcaaggcgaccgcggtgctgatgcaggacggcgagcggatcgagacagacatggttatcgtcggcatcggcatcgtgccggaaaccggccccctgatcgcggcgggcgccgctggcggcaatggtgtcgatgtggacgaatattgccgcaccagcctgccggatatctatgccgtaggcgattgcgcggcgcatgccaaccgctttgcccggggcgcgcaaattcgcctcgaatccgtgcagaacgccaatgatcaggcgaagacggcggtggcgcacatcatgggcaaggaagacccctatgacgcggtgccgtggttctggtccaatcaatatgacctgaaattgcagacggtcgggctgtcgaccggccatgaccagacgctattgcggggcgatccggcggcgcgcagcttttccgtgctatatttgaagggcgggaagctgatcgcgctagactgcgtcaatgcggtgaaggactatgtgcaggggcgcgcccatgtgctgtccggcgcggcgctggatcaggcgcaactggccgatccgtccgtgccgctcaaggaagtggggctggcttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z