BBa_K2088018
1
BBa_K2088018
J23100+B0030+pbaC
2016-10-08T11:00:00Z
2016-10-19T01:08:48Z
Uses BBa_K2088016 and BBa_K2088017.
This composite part includes optimized mhbI(BBa_K2088016) and mhbH(BBa_K2088017).
false
false
_2556_
29893
33751
9
false
None.
false
Xia Chen
component2522078
1
BBa_J23100
component2522080
1
BBa_B0030
component2522086
1
BBa_K2088005
annotation2522080
1
BBa_B0030
range2522080
1
36
50
annotation2522086
1
BBa_K2088005
range2522086
1
51
1414
annotation2522078
1
BBa_J23100
range2522078
1
1
35
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K2088005
1
BBa_K2088005
pbaC with double Terminator
2016-10-08T11:00:00Z
2016-10-19T12:17:49Z
This part is an optimization of BBa_K2088667.
This part includes optimized mhbD using Codon Optimization Tool of iDT and a designed rbs using RBS Calculator.
false
false
_2556_
33751
33751
9
false
An optimization of BBa_K2088667 with a designed rbs.
false
Xia Chen
annotation2521811
1
TAG Stop Codon
range2521811
1
1225
1227
annotation2521812
1
BBa_B0015
range2521812
1
1236
1364
annotation2521813
1
pbaC coding sequence
range2521813
1
1
1227
annotation2521810
1
ATG Start Codon
range2521810
1
1
3
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K2088018_sequence
1
ttgacggctagctcagtcctaggtacagtgctagcattaaagaggagaaaatgagctattatgacgttctgatcgtgggggccggccatgccggggcgcaagcagcgatttccctgcgccagcttgggttcgaaggatcggtcgcaatgatcggcgatgaaaaagacccgccctatgaacgtcccccgctgtccaaggaatatttcgcgggcgacaagaacttcgatcgcatcctcatcaggcccgccgccttctggggggaacggaaggtcgacatgctgctgggcaagcgcgtgaaacatgtcgatccgaccgccaaattcgtgacggcgggggatgaggaaatcggctatggttcgctgatctgggcgacgggcggcagtccgcgcatgttgacctgcaacggtgcggatgcgtccaacgtccatgcggtccgccgccgcgacgatgtcgacgccatgatggcgaagctggaccagatcgaccatgtgaccgtgatcggcggcggctatatcgggctggaggccgccgctgtcctcaccaagttcggcaagaaggtggtgctgctggaggcgctcgaccgggtactcgcccgcgtggcgggggaagatctttcgcgcttttacgaggccgagcatcgcgcccatggcgttgacctgcggaccggaacgaagatggactgcatcgaggtcagggacggcaaggcgaccgcggtgctgatgcaggacggcgagcggatcgagacagacatggttatcgtcggcatcggcatcgtgccggaaaccggccccctgatcgcggcgggcgccgctggcggcaatggtgtcgatgtggacgaatattgccgcaccagcctgccggatatctatgccgtaggcgattgcgcggcgcatgccaaccgctttgcccggggcgcgcaaattcgcctcgaatccgtgcagaacgccaatgatcaggcgaagacggcggtggcgcacatcatgggcaaggaagacccctatgacgcggtgccgtggttctggtccaatcaatatgacctgaaattgcagacggtcgggctgtcgaccggccatgaccagacgctattgcggggcgatccggcggcgcgcagcttttccgtgctatatttgaagggcgggaagctgatcgcgctagactgcgtcaatgcggtgaaggactatgtgcaggggcgcgcccatgtgctgtccggcgcggcgctggatcaggcgcaactggccgatccgtccgtgccgctcaaggaagtggggctggcttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2088005_sequence
1
atgagctattatgacgttctgatcgtgggggccggccatgccggggcgcaagcagcgatttccctgcgccagcttgggttcgaaggatcggtcgcaatgatcggcgatgaaaaagacccgccctatgaacgtcccccgctgtccaaggaatatttcgcgggcgacaagaacttcgatcgcatcctcatcaggcccgccgccttctggggggaacggaaggtcgacatgctgctgggcaagcgcgtgaaacatgtcgatccgaccgccaaattcgtgacggcgggggatgaggaaatcggctatggttcgctgatctgggcgacgggcggcagtccgcgcatgttgacctgcaacggtgcggatgcgtccaacgtccatgcggtccgccgccgcgacgatgtcgacgccatgatggcgaagctggaccagatcgaccatgtgaccgtgatcggcggcggctatatcgggctggaggccgccgctgtcctcaccaagttcggcaagaaggtggtgctgctggaggcgctcgaccgggtactcgcccgcgtggcgggggaagatctttcgcgcttttacgaggccgagcatcgcgcccatggcgttgacctgcggaccggaacgaagatggactgcatcgaggtcagggacggcaaggcgaccgcggtgctgatgcaggacggcgagcggatcgagacagacatggttatcgtcggcatcggcatcgtgccggaaaccggccccctgatcgcggcgggcgccgctggcggcaatggtgtcgatgtggacgaatattgccgcaccagcctgccggatatctatgccgtaggcgattgcgcggcgcatgccaaccgctttgcccggggcgcgcaaattcgcctcgaatccgtgcagaacgccaatgatcaggcgaagacggcggtggcgcacatcatgggcaaggaagacccctatgacgcggtgccgtggttctggtccaatcaatatgacctgaaattgcagacggtcgggctgtcgaccggccatgaccagacgctattgcggggcgatccggcggcgcgcagcttttccgtgctatatttgaagggcgggaagctgatcgcgctagactgcgtcaatgcggtgaaggactatgtgcaggggcgcgcccatgtgctgtccggcgcggcgctggatcaggcgcaactggccgatccgtccgtgccgctcaaggaagtggggctggcttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z