BBa_K2088005 1 BBa_K2088005 pbaC with double Terminator 2016-10-08T11:00:00Z 2016-10-19T12:17:49Z This part is an optimization of BBa_K2088667. This part includes optimized mhbD using Codon Optimization Tool of iDT and a designed rbs using RBS Calculator. false false _2556_ 33751 33751 9 false An optimization of BBa_K2088667 with a designed rbs. false Xia Chen annotation2521813 1 pbaC coding sequence range2521813 1 1 1227 annotation2521810 1 ATG Start Codon range2521810 1 1 3 annotation2521811 1 TAG Stop Codon range2521811 1 1225 1227 annotation2521812 1 BBa_B0015 range2521812 1 1236 1364 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K2088018 1 BBa_K2088018 J23100+B0030+pbaC 2016-10-08T11:00:00Z 2016-10-19T01:08:48Z Uses BBa_K2088016 and BBa_K2088017. This composite part includes optimized mhbI(BBa_K2088016) and mhbH(BBa_K2088017). false false _2556_ 29893 33751 9 false None. false Xia Chen component2522086 1 BBa_K2088005 component2522078 1 BBa_J23100 component2522080 1 BBa_B0030 annotation2522078 1 BBa_J23100 range2522078 1 1 35 annotation2522080 1 BBa_B0030 range2522080 1 36 50 annotation2522086 1 BBa_K2088005 range2522086 1 51 1414 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0030_sequence 1 attaaagaggagaaa BBa_K2088018_sequence 1 ttgacggctagctcagtcctaggtacagtgctagcattaaagaggagaaaatgagctattatgacgttctgatcgtgggggccggccatgccggggcgcaagcagcgatttccctgcgccagcttgggttcgaaggatcggtcgcaatgatcggcgatgaaaaagacccgccctatgaacgtcccccgctgtccaaggaatatttcgcgggcgacaagaacttcgatcgcatcctcatcaggcccgccgccttctggggggaacggaaggtcgacatgctgctgggcaagcgcgtgaaacatgtcgatccgaccgccaaattcgtgacggcgggggatgaggaaatcggctatggttcgctgatctgggcgacgggcggcagtccgcgcatgttgacctgcaacggtgcggatgcgtccaacgtccatgcggtccgccgccgcgacgatgtcgacgccatgatggcgaagctggaccagatcgaccatgtgaccgtgatcggcggcggctatatcgggctggaggccgccgctgtcctcaccaagttcggcaagaaggtggtgctgctggaggcgctcgaccgggtactcgcccgcgtggcgggggaagatctttcgcgcttttacgaggccgagcatcgcgcccatggcgttgacctgcggaccggaacgaagatggactgcatcgaggtcagggacggcaaggcgaccgcggtgctgatgcaggacggcgagcggatcgagacagacatggttatcgtcggcatcggcatcgtgccggaaaccggccccctgatcgcggcgggcgccgctggcggcaatggtgtcgatgtggacgaatattgccgcaccagcctgccggatatctatgccgtaggcgattgcgcggcgcatgccaaccgctttgcccggggcgcgcaaattcgcctcgaatccgtgcagaacgccaatgatcaggcgaagacggcggtggcgcacatcatgggcaaggaagacccctatgacgcggtgccgtggttctggtccaatcaatatgacctgaaattgcagacggtcgggctgtcgaccggccatgaccagacgctattgcggggcgatccggcggcgcgcagcttttccgtgctatatttgaagggcgggaagctgatcgcgctagactgcgtcaatgcggtgaaggactatgtgcaggggcgcgcccatgtgctgtccggcgcggcgctggatcaggcgcaactggccgatccgtccgtgccgctcaaggaagtggggctggcttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2088005_sequence 1 atgagctattatgacgttctgatcgtgggggccggccatgccggggcgcaagcagcgatttccctgcgccagcttgggttcgaaggatcggtcgcaatgatcggcgatgaaaaagacccgccctatgaacgtcccccgctgtccaaggaatatttcgcgggcgacaagaacttcgatcgcatcctcatcaggcccgccgccttctggggggaacggaaggtcgacatgctgctgggcaagcgcgtgaaacatgtcgatccgaccgccaaattcgtgacggcgggggatgaggaaatcggctatggttcgctgatctgggcgacgggcggcagtccgcgcatgttgacctgcaacggtgcggatgcgtccaacgtccatgcggtccgccgccgcgacgatgtcgacgccatgatggcgaagctggaccagatcgaccatgtgaccgtgatcggcggcggctatatcgggctggaggccgccgctgtcctcaccaagttcggcaagaaggtggtgctgctggaggcgctcgaccgggtactcgcccgcgtggcgggggaagatctttcgcgcttttacgaggccgagcatcgcgcccatggcgttgacctgcggaccggaacgaagatggactgcatcgaggtcagggacggcaaggcgaccgcggtgctgatgcaggacggcgagcggatcgagacagacatggttatcgtcggcatcggcatcgtgccggaaaccggccccctgatcgcggcgggcgccgctggcggcaatggtgtcgatgtggacgaatattgccgcaccagcctgccggatatctatgccgtaggcgattgcgcggcgcatgccaaccgctttgcccggggcgcgcaaattcgcctcgaatccgtgcagaacgccaatgatcaggcgaagacggcggtggcgcacatcatgggcaaggaagacccctatgacgcggtgccgtggttctggtccaatcaatatgacctgaaattgcagacggtcgggctgtcgaccggccatgaccagacgctattgcggggcgatccggcggcgcgcagcttttccgtgctatatttgaagggcgggaagctgatcgcgctagactgcgtcaatgcggtgaaggactatgtgcaggggcgcgcccatgtgctgtccggcgcggcgctggatcaggcgcaactggccgatccgtccgtgccgctcaaggaagtggggctggcttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z