LOCUS BBa_K2088616 3913 bp DNA linear 22-Nov-2024 DEFINITION MhbT+Mhbpt+GFP ACCESSION BBa_K2088616 VERSION BBa_K2088616.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 62..64 /label=ATG start codon misc_feature 1418..1420 /label=TAA stop codon CDS 62..1420 /label=MhbT coding region CDS 62..1420 /label=BBa_K2088666 stem_loop 1440..1483 /label=stem_loop misc_feature 1429..1508 /label=BBa_B0010 terminator 1429..1508 /label=BBa_B0010 stem_loop 1524..1543 /label=T7 TE misc_feature 1550..1550 /label=stop polyA_site 1544..1557 /label=polya misc_feature 1517..1557 /label=BBa_B0012 terminator 1517..1557 /label=BBa_B0012 terminator 1429..1557 /label=BBa_B0015 misc_feature 1..1557 /label=BBa_K2088671 stem_loop 1573..1592 /label=T7 TE misc_feature 1599..1599 /label=stop polyA_site 1593..1606 /label=polya misc_feature 1566..1606 /label=BBa_B0012 terminator 1566..1606 /label=BBa_B0012 stem_loop 1627..1649 /label=stem_loop misc_feature 1615..1660 /label=BBa_B0011 terminator 1615..1660 /label=BBa_B0011 terminator 1566..1660 /label=BBa_B0014 misc_feature 1667..1669 /label=ATG start codon misc_feature 2582..2584 /label=TGA stop codon CDS 1667..2584 /label=MhbR coding region CDS 1667..2584 /label=BBa_K2088011 promoter 2593..3027 /label=Mhbpt RBS 3028..3047 /label=native RBS CDS 3048..3765 /label=GFP misc_feature 3785..3913 /label=T1 terminator promoter 2593..3913 /label=BBa_K2088004 misc_feature 1566..3913 /label=BBa_K2088002 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgactcaa cgacttgaat tacagaagct gctcaacgcc gcgccggtcg gcgcccgaca 121 gtggcgcgtg attatctgct gtttcctggt ggtgatgctc gatggctttg ataccgccgc 181 gattggtttt atcgcgccgg atatccgcag ccactggcag ctcaccgccg gcgacctcgc 241 gccgttattt ggcgcgggat tgctgggcct gaccgccggg gcgctgctct gcggcccgct 301 ctccgaccgc tttggccgca aacgggtgat tgagttttgc gtcgccctgt tcggcctgtt 361 cagcctgctt tccgcgtttg cccccgatct gcaaatgctg gtgttcctgc gttttctgac 421 cggcctcggc ctcggcggcg caatgcctaa taccatcacc atgacctcgg aatatttgcc 481 tgcccggcgg cgcggggcgc tggtaaccct gatgttttgc gggttcaccc tcggttccgc 541 cttcggcggc gtggtcagcg cacagctggt gccgctgatc ggctggcacg gcattctggt 601 gctcggcggc gttttaccgt tgatactggc ggtggcgctg gtgcctctgc ttcccgagtc 661 tccgcgctgg cagatccgtc gccagctgcc gcaggcgacg atcgccagaa ccgtcggcgc 721 cattaccggc gagcgctata acgacacgca tttctggctt gatgaacctg ccgcaggggc 781 gaagggcagt atcagccagc tgttcgccgg gcgtcagttg gccatcacct taatgctatg 841 ggtggtgttc tttatgagcc tgctgattat ctatctgctc tccagctgga tgccgacgtt 901 gctaaaccat cggggcattg atttacagca cgcttcgtgg gtcaccgccg ccttccagat 961 tggcggcacc tttggcgcgc tgctgctcgg cgtgctgatg gatcgcttca atccctatcg 1021 ggtgctggcg ctgagctacg gcctcggggc ggtgtgtatc gtcatgattg gcctgagcga 1081 gaacggcctg tggctgatgg cgctggcgat tttcggcacc ggcatcggca tcagcggctc 1141 gcaggtgggg ctgaatgcgc taaccgcaac gctgtatccc acgcaaagcc gcgcgactgg 1201 cgtgagctgg tcaaacgcgg ttggccgctg cggggccatc gttggctccc tttccggcgg 1261 tgtgatgatg gcgatgaatt tctcattcga taccctgttt tttgttatcg ccgtcccggc 1321 cgttgtgagc gccgtgatgc ttatcctgct gacgcttgcc gtccgcaacc cgacttctgt 1381 acccgccgcg ctgccttccg caggcattgt gaataaataa tactagagcc aggcatcaaa 1441 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1501 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 1561 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa 1621 tataaaaagc cagattatta atccggcttt tttattattt tactagatgg cggactggac 1681 gcagaaagtg aagctgcacc atttacagat gctggtggcg ctgggggagc aaggcaatct 1741 cacgcaggtg gcgaaaatga tgaacattac tcagcccgcg ctctccaaat ggctcagcca 1801 gtttgaagac gaagtcggca tggttttgtt cgaacgccat agcaaaggcc tgcgcccgac 1861 cgaaggcggg aagctgctgc ttcagcacgc ccagcggctg attaacgata tgtcgcgttc 1921 gcagtatgag atcgagcgtt ttaagcaggg cggacaggtg ggcagcctga tgattggctg 1981 ttcgccggtg gcgaccgact gcgtctcgca ggcgatcctc gaactgctca aagagatgcc 2041 gaccctgcac ctgaatatcg aagagaaggt catgacgccg ctgcttaacg atctgctcgc 2101 cggcgtggtg gatgtggttg tcggacgcgt cggcggacgg gcgctggagc tgccgctgag 2161 ctaccgggtg ctctacaccg aaccggtctg ctttgtcgcc cgtcccgatc acccgctggc 2221 ccaaaaatcg cggttaagct gggccgatct ctccccctgg cgctggatcg tctggcccac 2281 cggcacgcca attcgcctga gcatcgacaa cgcgctggtg gataacggcg tgatgctgcc 2341 ggaaaacacc attgagtccg cctcgatgaa cgtcaccagc aacctactgc aaagcagcga 2401 tatggtctca attctctcct tgcgtctggc gcagcgctac gccgcccagc accagctggc 2461 gattcttaat ttgccgcgca ttgaacaaaa aggtagcgtt ggggtgttct ggcgaaaaag 2521 tgaaacgccc tcgctggcgc tgagccggtt tctgcactgc ctgtcgctgc aatctacgct 2581 ctgatactag aggctcgatc tcatactgcg aacgcgacat atcgttaatc agccgctggg 2641 cgtgctgaag cagcagcttc ccgccttcgg tcgggcgcag gcctttgcta tggcgttcga 2701 acaaaaccat gccgacttcg tcttcaaact ggctgagcca tttggagagc gcgggctgag 2761 taatgttcat cattttcgcc acctgcgtga gattgccttg ctcccccagc gccaccagca 2821 tctgtaaatg gtgcagcttc actttctgcg tccagtccgc cattagcgat aacctccagg 2881 ttatgtctat gcccgaaata ccattgtaca tttcattgcc tgagttacaa actcgcaaca 2941 taaaagaaat aaatacaaca cctaccccta cctgcactga ggcataacga tgactcaacg 3001 acttgaatta cagaagctgc tcaacgctac tagaggaaag aggagaaata ctagatgcgt 3061 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 3121 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 3181 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 3241 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 3301 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 3361 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 3421 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 3481 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 3541 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 3601 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 3661 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 3721 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag 3781 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcccttt cgttttatct 3841 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcacctttc ggtgggcctt 3901 tctgcgttta tat //