##gff-version 3
##sequence-region BBa_K2088616 1 3913
BBa_K2088616	.	engineered_region	1566	3913	.	+	0	ID=BBa_K2088002;Name=BBa_K2088002
BBa_K2088616	.	CDS	1667	2584	.	+	0	ID=BBa_K2088011;Name=BBa_K2088011;Parent=BBa_K2088002
BBa_K2088616	.	start_codon	1667	1669	.	+	0	ID=annotation2487578;Name=ATG start codon;Parent=BBa_K2088011
BBa_K2088616	.	CDS	1667	2584	.	+	0	ID=annotation2487580;Name=MhbR coding region;Parent=BBa_K2088011
BBa_K2088616	.	stop_codon	2582	2584	.	+	0	ID=annotation2487579;Name=TGA stop codon;Parent=BBa_K2088011
BBa_K2088616	.	promoter	2593	3913	.	+	0	ID=BBa_K2088004;Name=BBa_K2088004;Parent=BBa_K2088002
BBa_K2088616	.	CDS	3048	3765	.	+	0	ID=annotation2521997;Name=GFP;Parent=BBa_K2088004
BBa_K2088616	.	promoter	2593	3027	.	+	0	ID=annotation2489292;Name=Mhbpt;Parent=BBa_K2088004
BBa_K2088616	.	sequence_feature	3785	3913	.	+	0	ID=annotation2521999;Name=T1 terminator;Parent=BBa_K2088004
BBa_K2088616	.	ribosome_entry_site	3028	3047	.	+	0	ID=annotation2489293;Name=native RBS;Parent=BBa_K2088004
BBa_K2088616	.	terminator	1566	1660	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K2088002
BBa_K2088616	.	terminator	1566	1606	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K2088616	.	stem_loop	1573	1592	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2088616	.	engineered_region	1566	1606	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2088616	.	stop_codon	1599	1599	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2088616	.	polyA_site	1593	1606	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2088616	.	terminator	1615	1660	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K2088616	.	stem_loop	1627	1649	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K2088616	.	engineered_region	1615	1660	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K2088616	.	engineered_region	1	1557	.	+	0	ID=BBa_K2088671;Name=BBa_K2088671
BBa_K2088616	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K2088671
BBa_K2088616	.	terminator	1429	1557	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K2088671
BBa_K2088616	.	terminator	1517	1557	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2088616	.	stem_loop	1524	1543	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2088616	.	engineered_region	1517	1557	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2088616	.	stop_codon	1550	1550	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2088616	.	polyA_site	1544	1557	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2088616	.	terminator	1429	1508	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2088616	.	engineered_region	1429	1508	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2088616	.	stem_loop	1440	1483	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2088616	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K2088671
BBa_K2088616	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2088616	.	CDS	62	1420	.	+	0	ID=BBa_K2088666;Name=BBa_K2088666;Parent=BBa_K2088671
BBa_K2088616	.	stop_codon	1418	1420	.	+	0	ID=annotation2486846;Name=TAA stop codon  ;Parent=BBa_K2088666
BBa_K2088616	.	start_codon	62	64	.	+	0	ID=annotation2486845;Name=ATG start codon;Parent=BBa_K2088666
BBa_K2088616	.	CDS	62	1420	.	+	0	ID=annotation2486847;Name=MhbT coding region;Parent=BBa_K2088666
>BBa_K2088616
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgactcaacgacttgaa
ttacagaagctgctcaacgccgcgccggtcggcgcccgacagtggcgcgtgattatctgctgtttcctggtggtgatgc
tcgatggctttgataccgccgcgattggttttatcgcgccggatatccgcagccactggcagctcaccgccggcgacct
cgcgccgttatttggcgcgggattgctgggcctgaccgccggggcgctgctctgcggcccgctctccgaccgctttggc
cgcaaacgggtgattgagttttgcgtcgccctgttcggcctgttcagcctgctttccgcgtttgcccccgatctgcaaa
tgctggtgttcctgcgttttctgaccggcctcggcctcggcggcgcaatgcctaataccatcaccatgacctcggaata
tttgcctgcccggcggcgcggggcgctggtaaccctgatgttttgcgggttcaccctcggttccgccttcggcggcgtg
gtcagcgcacagctggtgccgctgatcggctggcacggcattctggtgctcggcggcgttttaccgttgatactggcgg
tggcgctggtgcctctgcttcccgagtctccgcgctggcagatccgtcgccagctgccgcaggcgacgatcgccagaac
cgtcggcgccattaccggcgagcgctataacgacacgcatttctggcttgatgaacctgccgcaggggcgaagggcagt
atcagccagctgttcgccgggcgtcagttggccatcaccttaatgctatgggtggtgttctttatgagcctgctgatta
tctatctgctctccagctggatgccgacgttgctaaaccatcggggcattgatttacagcacgcttcgtgggtcaccgc
cgccttccagattggcggcacctttggcgcgctgctgctcggcgtgctgatggatcgcttcaatccctatcgggtgctg
gcgctgagctacggcctcggggcggtgtgtatcgtcatgattggcctgagcgagaacggcctgtggctgatggcgctgg
cgattttcggcaccggcatcggcatcagcggctcgcaggtggggctgaatgcgctaaccgcaacgctgtatcccacgca
aagccgcgcgactggcgtgagctggtcaaacgcggttggccgctgcggggccatcgttggctccctttccggcggtgtg
atgatggcgatgaatttctcattcgataccctgttttttgttatcgccgtcccggccgttgtgagcgccgtgatgctta
tcctgctgacgcttgccgtccgcaacccgacttctgtacccgccgcgctgccttccgcaggcattgtgaataaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatt
ttactagatggcggactggacgcagaaagtgaagctgcaccatttacagatgctggtggcgctgggggagcaaggcaat
ctcacgcaggtggcgaaaatgatgaacattactcagcccgcgctctccaaatggctcagccagtttgaagacgaagtcg
gcatggttttgttcgaacgccatagcaaaggcctgcgcccgaccgaaggcgggaagctgctgcttcagcacgcccagcg
gctgattaacgatatgtcgcgttcgcagtatgagatcgagcgttttaagcagggcggacaggtgggcagcctgatgatt
ggctgttcgccggtggcgaccgactgcgtctcgcaggcgatcctcgaactgctcaaagagatgccgaccctgcacctga
atatcgaagagaaggtcatgacgccgctgcttaacgatctgctcgccggcgtggtggatgtggttgtcggacgcgtcgg
cggacgggcgctggagctgccgctgagctaccgggtgctctacaccgaaccggtctgctttgtcgcccgtcccgatcac
ccgctggcccaaaaatcgcggttaagctgggccgatctctccccctggcgctggatcgtctggcccaccggcacgccaa
ttcgcctgagcatcgacaacgcgctggtggataacggcgtgatgctgccggaaaacaccattgagtccgcctcgatgaa
cgtcaccagcaacctactgcaaagcagcgatatggtctcaattctctccttgcgtctggcgcagcgctacgccgcccag
caccagctggcgattcttaatttgccgcgcattgaacaaaaaggtagcgttggggtgttctggcgaaaaagtgaaacgc
cctcgctggcgctgagccggtttctgcactgcctgtcgctgcaatctacgctctgatactagaggctcgatctcatact
gcgaacgcgacatatcgttaatcagccgctgggcgtgctgaagcagcagcttcccgccttcggtcgggcgcaggccttt
gctatggcgttcgaacaaaaccatgccgacttcgtcttcaaactggctgagccatttggagagcgcgggctgagtaatg
ttcatcattttcgccacctgcgtgagattgccttgctcccccagcgccaccagcatctgtaaatggtgcagcttcactt
tctgcgtccagtccgccattagcgataacctccaggttatgtctatgcccgaaataccattgtacatttcattgcctga
gttacaaactcgcaacataaaagaaataaatacaacacctacccctacctgcactgaggcataacgatgactcaacgac
ttgaattacagaagctgctcaacgctactagaggaaagaggagaaatactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggccctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcacctttcggtgggcctttctgcgtttatat