BBa_K2088616
type: ComponentDefinition
persistent identity: BBa K2088616
display id: BBa_K2088616
version: 1
was generated by: iGEM to SBOL conversion
description: MhbT+Mhbpt+GFP
created: 2016-10-08T11:00:00Z
modified: 2016-10-19T02:53:40Z
mutable provenance: no
owned by: james
owned by: myers
mutable description: This part includes optimized mhbM using Codon Optimization Tool of IDT and a designed rbs using RBS Calculator.
discontinued: false
dominant: false
experience: experience/None
group u list: _2556_
m user id: 29893
owner id: 33447
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: chenrui qin
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K2088671
component: BBa_K2088002
sequence annotation: BBa_K2088671
sequence annotation: BBa_K2088002
sequence: https://synbiohub.org/public/igem/BBa_K2088616_sequence/1

BBa_K2088671
type: Component
persistent identity: component2522623
display id: component2522623
version: 1
top level: BBa_K2088616
definition: BBa_K2088671
access: public

BBa_K2088002
type: Component
persistent identity: component2522640
display id: component2522640
version: 1
top level: BBa_K2088616
definition: BBa_K2088002
access: public

BBa_K2088671
type: SequenceAnnotation
persistent identity: annotation2522623
display id: annotation2522623
version: 1
top level: BBa_K2088616
location: https://synbiohub.org/public/igem/BBa_K2088616/annotation2522623/range2522623/1
component: BBa_K2088671

https://synbiohub.org/public/igem/BBa_K2088616/annotation2522623/range2522623/1
type: Range
persistent identity: range2522623
display id: range2522623
version: 1
top level: BBa_K2088616
direction: direction/reverse
start: 1
end: 1557
orientation: inline

BBa_K2088002
type: SequenceAnnotation
persistent identity: annotation2522640
display id: annotation2522640
version: 1
top level: BBa_K2088616
location: https://synbiohub.org/public/igem/BBa_K2088616/annotation2522640/range2522640/1
component: BBa_K2088002

https://synbiohub.org/public/igem/BBa_K2088616/annotation2522640/range2522640/1
type: Range
persistent identity: range2522640
display id: range2522640
version: 1
top level: BBa_K2088616
direction: direction/reverse
start: 1566
end: 3913
orientation: inline

BBa_K2088002
type: ComponentDefinition
persistent identity: BBa K2088002
display id: BBa_K2088002
version: 1
was generated by: iGEM to SBOL conversion
description: dT+mhbR+mhbPt+GFP
created: 2016-10-01T11:00:00Z
modified: 2016-10-19T01:59:53Z
mutable provenance: PbaC comes from Sphingobium wenxiniae JZ-1, and it exists on the Scaffold 12, instead of the vector of pbaA1A2B.Sphingobium wenxiniae JZ-1 utilizes a wide range of pyrethroids and their metabolic product, 3-phenoxybenzoate, as sources of carbon and energy. GenBank: KJ009325.1 reference: A Novel Angular Dioxygenase Gene Cluster Encoding 3-Phenoxybenzoate 1=,2=-Dioxygenase in Sphingobium wenxiniae JZ-1, Chenghong Wang, Qing Chen, Rui Wang, Chao Shi, Xin Yan, Jian He, Qing Hong, Shunpeng Li Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
owned by: james
owned by: myers
mutable description: PbaC is a 44-kDa protein that consists of 408 amino acids and contains a consensus motif for a flavin adenine dinucleotide-binding (ADP-binding) site (GXGX2GX3A X6G)。Existing in Sphingobium wenxiniae JZ-1, it???s a glutathione reductase (GR)-type reductase gene. Though pbaC is the important gene to degrade 3-PBA as the first step, the transcriptional level of pbaC was far less than that of other genes like pbaA1A2B since it is not located in the immediate vicinity of pbaA1A2B. However, with the optimization of our experiment, the efficience of degradation on 3-pba has been improved.
discontinued: false
dominant: false
experience: experience/None
group u list: _2556_
m user id: 29893
owner id: 33723
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: we have used diffrerent promotors and RBS to test the degrading efficience of pbaC. And we design the sequence as the part submission requests.
star: false
creator: Yuxin He
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0014
component: BBa_K2088004
component: BBa_K2088011
sequence annotation: BBa_B0014
sequence annotation: BBa_K2088011
sequence annotation: BBa_K2088004
sequence: https://synbiohub.org/public/igem/BBa_K2088002_sequence/1

BBa_B0014
type: Component
persistent identity: component2522261
display id: component2522261
version: 1
top level: BBa_K2088002
definition: BBa_B0014
access: public

BBa_K2088004
type: Component
persistent identity: component2522270
display id: component2522270
version: 1
top level: BBa_K2088002
definition: BBa_K2088004
access: public

BBa_K2088011
type: Component
persistent identity: component2522265
display id: component2522265
version: 1
top level: BBa_K2088002
definition: BBa_K2088011
access: public

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2522261
display id: annotation2522261
version: 1
top level: BBa_K2088002
location: https://synbiohub.org/public/igem/BBa_K2088002/annotation2522261/range2522261/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2088002/annotation2522261/range2522261/1
type: Range
persistent identity: range2522261
display id: range2522261
version: 1
top level: BBa_K2088002
direction: direction/reverse
start: 1
end: 95
orientation: inline

BBa_K2088011
type: SequenceAnnotation
persistent identity: annotation2522265
display id: annotation2522265
version: 1
top level: BBa_K2088002
location: https://synbiohub.org/public/igem/BBa_K2088002/annotation2522265/range2522265/1
component: BBa_K2088011

https://synbiohub.org/public/igem/BBa_K2088002/annotation2522265/range2522265/1
type: Range
persistent identity: range2522265
display id: range2522265
version: 1
top level: BBa_K2088002
direction: direction/reverse
start: 102
end: 1019
orientation: inline

BBa_K2088004
type: SequenceAnnotation
persistent identity: annotation2522270
display id: annotation2522270
version: 1
top level: BBa_K2088002
location: https://synbiohub.org/public/igem/BBa_K2088002/annotation2522270/range2522270/1
component: BBa_K2088004

https://synbiohub.org/public/igem/BBa_K2088002/annotation2522270/range2522270/1
type: Range
persistent identity: range2522270
display id: range2522270
version: 1
top level: BBa_K2088002
direction: direction/reverse
start: 1028
end: 2348
orientation: inline

BBa_K2088671
type: ComponentDefinition
persistent identity: BBa K2088671
display id: BBa_K2088671
version: 1
was generated by: iGEM to SBOL conversion
description: J23100+B0034+mhbT+dT
created: 2016-10-08T11:00:00Z
modified: 2016-10-14T08:56:44Z
mutable provenance: This is a compostie part including a putative 3-hydroxybenzoate transporter gene(mhbT) from Klebsiella pneumoniae strain M5a1.
owned by: james
owned by: myers
mutable description: This composite part includes a constitutive promoter (BBa_J23100), a RBS(BBa_B0034), a putative 3-hydroxybenzoate transporter gene(mhbT) and the double terminator (BBa_B0015). The gene MhbT is a transport protein which is located on the cytomembrane. It can transport the 3-HBA into the cell.By sequence comparison. This composite part can be used in degradation system of 3-HBA, and can also be used in induction system whose derivant is 3-HBA.
discontinued: false
dominant: false
experience: experience/None
group u list: _2556_
m user id: 33751
owner id: 33723
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Uses J23100, a strong Anderson Promoter, to produce high levels of expression of mhbT.
star: false
creator: Yuxin He
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0015
component: BBa_K2088666
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K2088666
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K2088671_sequence/1

BBa_B0015
type: Component
persistent identity: component2488838
display id: component2488838
version: 1
top level: BBa_K2088671
definition: BBa_B0015
access: public

BBa_K2088666
type: Component
persistent identity: component2488831
display id: component2488831
version: 1
top level: BBa_K2088671
definition: T
access: public

BBa_B0034
type: Component
persistent identity: component2488827
display id: component2488827
version: 1
top level: BBa_K2088671
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2488825
display id: component2488825
version: 1
top level: BBa_K2088671
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2488825
display id: annotation2488825
version: 1
top level: BBa_K2088671
location: https://synbiohub.org/public/igem/BBa_K2088671/annotation2488825/range2488825/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K2088671/annotation2488825/range2488825/1
type: Range
persistent identity: range2488825
display id: range2488825
version: 1
top level: BBa_K2088671
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2488827
display id: annotation2488827
version: 1
top level: BBa_K2088671
location: https://synbiohub.org/public/igem/BBa_K2088671/annotation2488827/range2488827/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2088671/annotation2488827/range2488827/1
type: Range
persistent identity: range2488827
display id: range2488827
version: 1
top level: BBa_K2088671
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K2088666
type: SequenceAnnotation
persistent identity: annotation2488831
display id: annotation2488831
version: 1
top level: BBa_K2088671
location: https://synbiohub.org/public/igem/BBa_K2088671/annotation2488831/range2488831/1
component: BBa_K2088666

https://synbiohub.org/public/igem/BBa_K2088671/annotation2488831/range2488831/1
type: Range
persistent identity: range2488831
display id: range2488831
version: 1
top level: BBa_K2088671
direction: direction/reverse
start: 62
end: 1420
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2488838
display id: annotation2488838
version: 1
top level: BBa_K2088671
location: https://synbiohub.org/public/igem/BBa_K2088671/annotation2488838/range2488838/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2088671/annotation2488838/range2488838/1
type: Range
persistent identity: range2488838
display id: range2488838
version: 1
top level: BBa_K2088671
direction: direction/reverse
start: 1429
end: 1557
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_K2088004
type: ComponentDefinition
persistent identity: BBa K2088004
display id: BBa_K2088004
version: 1
was generated by: iGEM to SBOL conversion
description: Mhbpt+GFP
created: 2016-10-08T11:00:00Z
modified: 2016-10-19T12:37:39Z
mutable provenance: PbaC comes from Sphingobium wenxiniae JZ-1, and it exists on the Scaffold 12, instead of the vector of pbaA1A2B.Sphingobium wenxiniae JZ-1 utilizes a wide range of pyrethroids and their metabolic product, 3-phenoxybenzoate, as sources of carbon and energy.
owned by: james
owned by: myers
mutable description: It???s a codon-optimized gene of pbaC(BBa_K2088003), and it can be used with provided standard restriction site (EcoRI,XbaI,SpeI,PstI) without destructing the functional region of pbaC. PbaC is the phenoxybenzoic acid dioxygenase reductase on Sphingobium wenxiniae's genomic DNA and it codes a 44-kDa protein that consists of 408 amino acids and contains a consensus motif for a flavin adenine dinucleotide-binding (ADP-binding) site (GXGX2GX3AX6G)。Existing in Sphingobium wenxiniae JZ-1, it???s a glutathione reductase (GR)-type reductase gene. Though pbaC is the important gene to degrade 3-PBA as the first step, the transcriptional level of pbaC was far less than that of other genes like pbaA1A2B since it is not located in the immediate vicinity of pbaA1A2B. However, with the optimization of our experiment, the efficience of degradation on 3-pba has been improved.
discontinued: false
dominant: false
experience: experience/None
group u list: _2556_
m user id: 29893
owner id: 33723
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We optimaized the codon region of pbaC for a more convient use if other teams want to use it.
star: false
creator: Yuxin He
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Mhbpt
sequence annotation: native RBS
sequence annotation: GFP
sequence annotation: T1 terminator
sequence: https://synbiohub.org/public/igem/BBa_K2088004_sequence/1

Mhbpt
type: SequenceAnnotation
persistent identity: annotation2489292
display id: annotation2489292
version: 1
top level: BBa_K2088004
location: https://synbiohub.org/public/igem/BBa_K2088004/annotation2489292/range2489292/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K2088004/annotation2489292/range2489292/1
type: Range
persistent identity: range2489292
display id: range2489292
version: 1
top level: BBa_K2088004
direction: direction/forward
start: 1
end: 435
orientation: inline

native RBS
type: SequenceAnnotation
persistent identity: annotation2489293
display id: annotation2489293
version: 1
top level: BBa_K2088004
location: https://synbiohub.org/public/igem/BBa_K2088004/annotation2489293/range2489293/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K2088004/annotation2489293/range2489293/1
type: Range
persistent identity: range2489293
display id: range2489293
version: 1
top level: BBa_K2088004
direction: direction/forward
start: 436
end: 455
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2521997
display id: annotation2521997
version: 1
top level: BBa_K2088004
location: https://synbiohub.org/public/igem/BBa_K2088004/annotation2521997/range2521997/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2088004/annotation2521997/range2521997/1
type: Range
persistent identity: range2521997
display id: range2521997
version: 1
top level: BBa_K2088004
direction: direction/forward
start: 456
end: 1173
orientation: inline

T1 terminator
type: SequenceAnnotation
persistent identity: annotation2521999
display id: annotation2521999
version: 1
top level: BBa_K2088004
location: https://synbiohub.org/public/igem/BBa_K2088004/annotation2521999/range2521999/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K2088004/annotation2521999/range2521999/1
type: Range
persistent identity: range2521999
display id: range2521999
version: 1
top level: BBa_K2088004
direction: direction/forward
start: 1193
end: 1321
orientation: inline

BBa_K2088011
type: ComponentDefinition
persistent identity: BBa K2088011
display id: BBa_K2088011
version: 1
was generated by: iGEM to SBOL conversion
description: MhbR: a protein that combines the 3HBA to induce the promoter Mhbpt.
created: 2016-10-07T11:00:00Z
modified: 2016-10-14T07:22:34Z
mutable provenance: The gene of MhbR can be gotten from the bacteria M5a1 using PCR.The sequence of the gene comes from GenBank https://www.ncbi.nlm.nih.gov/nuccore/EU165545.1
owned by: james
owned by: myers
mutable description: MhbR is a protein that can combine the 3HBA to induce the promoter Mhbpt.MhbR can be used in the induction system and the system will be induced when both of the MhbR and the 3-HBA exist. It can also be used as a section of the detection device when we need to degrade the 3-HBA.
discontinued: false
dominant: false
experience: experience/None
group u list: _2556_
m user id: 33751
owner id: 29893
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Shi Kejian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: MhbR coding region
sequence annotation: ATG start codon
sequence annotation: TGA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K2088011_sequence/1

MhbR coding region
type: SequenceAnnotation
persistent identity: annotation2487580
display id: annotation2487580
version: 1
top level: BBa_K2088011
location: https://synbiohub.org/public/igem/BBa_K2088011/annotation2487580/range2487580/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K2088011/annotation2487580/range2487580/1
type: Range
persistent identity: range2487580
display id: range2487580
version: 1
top level: BBa_K2088011
direction: direction/forward
start: 1
end: 918
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2487578
display id: annotation2487578
version: 1
top level: BBa_K2088011
location: https://synbiohub.org/public/igem/BBa_K2088011/annotation2487578/range2487578/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K2088011/annotation2487578/range2487578/1
type: Range
persistent identity: range2487578
display id: range2487578
version: 1
top level: BBa_K2088011
direction: direction/forward
start: 1
end: 3
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2487579
display id: annotation2487579
version: 1
top level: BBa_K2088011
location: https://synbiohub.org/public/igem/BBa_K2088011/annotation2487579/range2487579/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K2088011/annotation2487579/range2487579/1
type: Range
persistent identity: range2487579
display id: range2487579
version: 1
top level: BBa_K2088011
direction: direction/forward
start: 916
end: 918
orientation: inline

T
type: ComponentDefinition
persistent identity: BBa K2088666
display id: BBa_K2088666
version: 1
was generated by: iGEM to SBOL conversion
description: MhbT is responsible for the uptake of 3-hydroxybenzoate
created: 2016-10-06T11:00:00Z
modified: 2016-10-08T03:01:00Z
mutable provenance: We get the part from the bacteria M5a1, and the sequence of the gene was found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/AY648560.1
owned by: james
owned by: myers
mutable description: MhbT is a transport protein which is located on the cytomembrane. It can transport the 3-HBA into the cell.By sequence comparison, MhbT exhibited 42% identity with the 4-hydroxybenzoate and protocatechuate transporter PcaK from P.putida, 27% identity with the benzoate transporter BenK from Acinetobacter sp. Using the MhbT-GFP fusion proteins , we can find that MhbT is located to the periphery of cells. It can be used in degradation system of 3-HBA, and can also be used in induction system whose derivant is 3-HBA.
discontinued: false
dominant: false
experience: experience/None
group u list: _2556_
m user id: 29893
owner id: 29893
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Shi Kejian
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: MhbT coding region
sequence annotation: ATG start codon
sequence annotation: TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K2088666_sequence/1

MhbT coding region
type: SequenceAnnotation
persistent identity: annotation2486847
display id: annotation2486847
version: 1
top level: T
location: https://synbiohub.org/public/igem/BBa_K2088666/annotation2486847/range2486847/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K2088666/annotation2486847/range2486847/1
type: Range
persistent identity: range2486847
display id: range2486847
version: 1
top level: T
direction: direction/forward
start: 1
end: 1359
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2486845
display id: annotation2486845
version: 1
top level: T
location: https://synbiohub.org/public/igem/BBa_K2088666/annotation2486845/range2486845/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K2088666/annotation2486845/range2486845/1
type: Range
persistent identity: range2486845
display id: range2486845
version: 1
top level: T
direction: direction/forward
start: 1
end: 3
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2486846
display id: annotation2486846
version: 1
top level: T
location: https://synbiohub.org/public/igem/BBa_K2088666/annotation2486846/range2486846/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K2088666/annotation2486846/range2486846/1
type: Range
persistent identity: range2486846
display id: range2486846
version: 1
top level: T
direction: direction/forward
start: 1357
end: 1359
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2088616_sequence/1
type: Sequence
persistent identity: BBa K2088616 sequence
display id: BBa_K2088616_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgactcaacgacttgaattacagaagctgctcaacgccgcgccggtcggcgcccgacagtggcgcgtgattatctgctgtttcctggtggtgatgctcgatggctttgataccgccgcgattggttttatcgcgccggatatccgcagccactggcagctcaccgccggcgacctcgcgccgttatttggcgcgggattgctgggcctgaccgccggggcgctgctctgcggcccgctctccgaccgctttggccgcaaacgggtgattgagttttgcgtcgccctgttcggcctgttcagcctgctttccgcgtttgcccccgatctgcaaatgctggtgttcctgcgttttctgaccggcctcggcctcggcggcgcaatgcctaataccatcaccatgacctcggaatatttgcctgcccggcggcgcggggcgctggtaaccctgatgttttgcgggttcaccctcggttccgccttcggcggcgtggtcagcgcacagctggtgccgctgatcggctggcacggcattctggtgctcggcggcgttttaccgttgatactggcggtggcgctggtgcctctgcttcccgagtctccgcgctggcagatccgtcgccagctgccgcaggcgacgatcgccagaaccgtcggcgccattaccggcgagcgctataacgacacgcatttctggcttgatgaacctgccgcaggggcgaagggcagtatcagccagctgttcgccgggcgtcagttggccatcaccttaatgctatgggtggtgttctttatgagcctgctgattatctatctgctctccagctggatgccgacgttgctaaaccatcggggcattgatttacagcacgcttcgtgggtcaccgccgccttccagattggcggcacctttggcgcgctgctgctcggcgtgctgatggatcgcttcaatccctatcgggtgctggcgctgagctacggcctcggggcggtgtgtatcgtcatgattggcctgagcgagaacggcctgtggctgatggcgctggcgattttcggcaccggcatcggcatcagcggctcgcaggtggggctgaatgcgctaaccgcaacgctgtatcccacgcaaagccgcgcgactggcgtgagctggtcaaacgcggttggccgctgcggggccatcgttggctccctttccggcggtgtgatgatggcgatgaatttctcattcgataccctgttttttgttatcgccgtcccggccgttgtgagcgccgtgatgcttatcctgctgacgcttgccgtccgcaacccgacttctgtacccgccgcgctgccttccgcaggcattgtgaataaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagatggcggactggacgcagaaagtgaagctgcaccatttacagatgctggtggcgctgggggagcaaggcaatctcacgcaggtggcgaaaatgatgaacattactcagcccgcgctctccaaatggctcagccagtttgaagacgaagtcggcatggttttgttcgaacgccatagcaaaggcctgcgcccgaccgaaggcgggaagctgctgcttcagcacgcccagcggctgattaacgatatgtcgcgttcgcagtatgagatcgagcgttttaagcagggcggacaggtgggcagcctgatgattggctgttcgccggtggcgaccgact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2088002_sequence/1
type: Sequence
persistent identity: BBa K2088002 sequence
display id: BBa_K2088002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagatggcggactggacgcagaaagtgaagctgcaccatttacagatgctggtggcgctgggggagcaaggcaatctcacgcaggtggcgaaaatgatgaacattactcagcccgcgctctccaaatggctcagccagtttgaagacgaagtcggcatggttttgttcgaacgccatagcaaaggcctgcgcccgaccgaaggcgggaagctgctgcttcagcacgcccagcggctgattaacgatatgtcgcgttcgcagtatgagatcgagcgttttaagcagggcggacaggtgggcagcctgatgattggctgttcgccggtggcgaccgactgcgtctcgcaggcgatcctcgaactgctcaaagagatgccgaccctgcacctgaatatcgaagagaaggtcatgacgccgctgcttaacgatctgctcgccggcgtggtggatgtggttgtcggacgcgtcggcggacgggcgctggagctgccgctgagctaccgggtgctctacaccgaaccggtctgctttgtcgcccgtcccgatcacccgctggcccaaaaatcgcggttaagctgggccgatctctccccctggcgctggatcgtctggcccaccggcacgccaattcgcctgagcatcgacaacgcgctggtggataacggcgtgatgctgccggaaaacaccattgagtccgcctcgatgaacgtcaccagcaacctactgcaaagcagcgatatggtctcaattctctccttgcgtctggcgcagcgctacgccgcccagcaccagctggcgattcttaatttgccgcgcattgaacaaaaaggtagcgttggggtgttctggcgaaaaagtgaaacgccctcgctggcgctgagccggtttctgcactgcctgtcgctgcaatctacgctctgatactagaggctcgatctcatactgcgaacgcgacatatcgttaatcagccgctgggcgtgctgaagcagcagcttcccgccttcggtcgggcgcaggcctttgctatggcgttcgaacaaaaccatgccgacttcgtcttcaaactggctgagccatttggagagcgcgggctgagtaatgttcatcattttcgccacctgcgtgagattgccttgctcccccagcgccaccagcatctgtaaatggtgcagcttcactttctgcgtccagtccgccattagcgataacctccaggttatgtctatgcccgaaataccattgtacatttcattgcctgagttacaaactcgcaacataaaagaaataaatacaacacctacccctacctgcactgaggcataacgatgactcaacgacttgaattacagaagctgctcaacgctactagaggaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2088671_sequence/1
type: Sequence
persistent identity: BBa K2088671 sequence
display id: BBa_K2088671_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgactcaacgacttgaattacagaagctgctcaacgccgcgccggtcggcgcccgacagtggcgcgtgattatctgctgtttcctggtggtgatgctcgatggctttgataccgccgcgattggttttatcgcgccggatatccgcagccactggcagctcaccgccggcgacctcgcgccgttatttggcgcgggattgctgggcctgaccgccggggcgctgctctgcggcccgctctccgaccgctttggccgcaaacgggtgattgagttttgcgtcgccctgttcggcctgttcagcctgctttccgcgtttgcccccgatctgcaaatgctggtgttcctgcgttttctgaccggcctcggcctcggcggcgcaatgcctaataccatcaccatgacctcggaatatttgcctgcccggcggcgcggggcgctggtaaccctgatgttttgcgggttcaccctcggttccgccttcggcggcgtggtcagcgcacagctggtgccgctgatcggctggcacggcattctggtgctcggcggcgttttaccgttgatactggcggtggcgctggtgcctctgcttcccgagtctccgcgctggcagatccgtcgccagctgccgcaggcgacgatcgccagaaccgtcggcgccattaccggcgagcgctataacgacacgcatttctggcttgatgaacctgccgcaggggcgaagggcagtatcagccagctgttcgccgggcgtcagttggccatcaccttaatgctatgggtggtgttctttatgagcctgctgattatctatctgctctccagctggatgccgacgttgctaaaccatcggggcattgatttacagcacgcttcgtgggtcaccgccgccttccagattggcggcacctttggcgcgctgctgctcggcgtgctgatggatcgcttcaatccctatcgggtgctggcgctgagctacggcctcggggcggtgtgtatcgtcatgattggcctgagcgagaacggcctgtggctgatggcgctggcgattttcggcaccggcatcggcatcagcggctcgcaggtggggctgaatgcgctaaccgcaacgctgtatcccacgcaaagccgcgcgactggcgtgagctggtcaaacgcggttggccgctgcggggccatcgttggctccctttccggcggtgtgatgatggcgatgaatttctcattcgataccctgttttttgttatcgccgtcccggccgttgtgagcgccgtgatgcttatcctgctgacgcttgccgtccgcaacccgacttctgtacccgccgcgctgccttccgcaggcattgtgaataaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2088004_sequence/1
type: Sequence
persistent identity: BBa K2088004 sequence
display id: BBa_K2088004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcgatctcatactgcgaacgcgacatatcgttaatcagccgctgggcgtgctgaagcagcagcttcccgccttcggtcgggcgcaggcctttgctatggcgttcgaacaaaaccatgccgacttcgtcttcaaactggctgagccatttggagagcgcgggctgagtaatgttcatcattttcgccacctgcgtgagattgccttgctcccccagcgccaccagcatctgtaaatggtgcagcttcactttctgcgtccagtccgccattagcgataacctccaggttatgtctatgcccgaaataccattgtacatttcattgcctgagttacaaactcgcaacataaaagaaataaatacaacacctacccctacctgcactgaggcataacgatgactcaacgacttgaattacagaagctgctcaacgctactagaggaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctttcggtgggcctttctgcgtttatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2088011_sequence/1
type: Sequence
persistent identity: BBa K2088011 sequence
display id: BBa_K2088011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcggactggacgcagaaagtgaagctgcaccatttacagatgctggtggcgctgggggagcaaggcaatctcacgcaggtggcgaaaatgatgaacattactcagcccgcgctctccaaatggctcagccagtttgaagacgaagtcggcatggttttgttcgaacgccatagcaaaggcctgcgcccgaccgaaggcgggaagctgctgcttcagcacgcccagcggctgattaacgatatgtcgcgttcgcagtatgagatcgagcgttttaagcagggcggacaggtgggcagcctgatgattggctgttcgccggtggcgaccgactgcgtctcgcaggcgatcctcgaactgctcaaagagatgccgaccctgcacctgaatatcgaagagaaggtcatgacgccgctgcttaacgatctgctcgccggcgtggtggatgtggttgtcggacgcgtcggcggacgggcgctggagctgccgctgagctaccgggtgctctacaccgaaccggtctgctttgtcgcccgtcccgatcacccgctggcccaaaaatcgcggttaagctgggccgatctctccccctggcgctggatcgtctggcccaccggcacgccaattcgcctgagcatcgacaacgcgctggtggataacggcgtgatgctgccggaaaacaccattgagtccgcctcgatgaacgtcaccagcaacctactgcaaagcagcgatatggtctcaattctctccttgcgtctggcgcagcgctacgccgcccagcaccagctggcgattcttaatttgccgcgcattgaacaaaaaggtagcgttggggtgttctggcgaaaaagtgaaacgccctcgctggcgctgagccggtttctgcactgcctgtcgctgcaatctacgctctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2088666_sequence/1
type: Sequence
persistent identity: BBa K2088666 sequence
display id: BBa_K2088666_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactcaacgacttgaattacagaagctgctcaacgccgcgccggtcggcgcccgacagtggcgcgtgattatctgctgtttcctggtggtgatgctcgatggctttgataccgccgcgattggttttatcgcgccggatatccgcagccactggcagctcaccgccggcgacctcgcgccgttatttggcgcgggattgctgggcctgaccgccggggcgctgctctgcggcccgctctccgaccgctttggccgcaaacgggtgattgagttttgcgtcgccctgttcggcctgttcagcctgctttccgcgtttgcccccgatctgcaaatgctggtgttcctgcgttttctgaccggcctcggcctcggcggcgcaatgcctaataccatcaccatgacctcggaatatttgcctgcccggcggcgcggggcgctggtaaccctgatgttttgcgggttcaccctcggttccgccttcggcggcgtggtcagcgcacagctggtgccgctgatcggctggcacggcattctggtgctcggcggcgttttaccgttgatactggcggtggcgctggtgcctctgcttcccgagtctccgcgctggcagatccgtcgccagctgccgcaggcgacgatcgccagaaccgtcggcgccattaccggcgagcgctataacgacacgcatttctggcttgatgaacctgccgcaggggcgaagggcagtatcagccagctgttcgccgggcgtcagttggccatcaccttaatgctatgggtggtgttctttatgagcctgctgattatctatctgctctccagctggatgccgacgttgctaaaccatcggggcattgatttacagcacgcttcgtgggtcaccgccgccttccagattggcggcacctttggcgcgctgctgctcggcgtgctgatggatcgcttcaatccctatcgggtgctggcgctgagctacggcctcggggcggtgtgtatcgtcatgattggcctgagcgagaacggcctgtggctgatggcgctggcgattttcggcaccggcatcggcatcagcggctcgcaggtggggctgaatgcgctaaccgcaacgctgtatcccacgcaaagccgcgcgactggcgtgagctggtcaaacgcggttggccgctgcggggccatcgttggctccctttccggcggtgtgatgatggcgatgaatttctcattcgataccctgttttttgttatcgccgtcccggccgttgtgagcgccgtgatgcttatcctgctgacgcttgccgtccgcaacccgacttctgtacccgccgcgctgccttccgcaggcattgtgaataaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z