BBa_K2088666 1 T MhbT is responsible for the uptake of 3-hydroxybenzoate 2016-10-06T11:00:00Z 2016-10-08T03:01:00Z We get the part from the bacteria M5a1, and the sequence of the gene was found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/AY648560.1 MhbT is a transport protein which is located on the cytomembrane. It can transport the 3-HBA into the cell.By sequence comparison, MhbT exhibited 42% identity with the 4-hydroxybenzoate and protocatechuate transporter PcaK from P.putida, 27% identity with the benzoate transporter BenK from Acinetobacter sp. Using the MhbT-GFP fusion proteins , we can find that MhbT is located to the periphery of cells. It can be used in degradation system of 3-HBA, and can also be used in induction system whose derivant is 3-HBA. false false _2556_ 29893 29893 9 false None false Shi Kejian annotation2486847 1 MhbT coding region range2486847 1 1 1359 annotation2486845 1 ATG start codon range2486845 1 1 3 annotation2486846 1 TAA stop codon range2486846 1 1357 1359 BBa_K2088666_sequence 1 atgactcaacgacttgaattacagaagctgctcaacgccgcgccggtcggcgcccgacagtggcgcgtgattatctgctgtttcctggtggtgatgctcgatggctttgataccgccgcgattggttttatcgcgccggatatccgcagccactggcagctcaccgccggcgacctcgcgccgttatttggcgcgggattgctgggcctgaccgccggggcgctgctctgcggcccgctctccgaccgctttggccgcaaacgggtgattgagttttgcgtcgccctgttcggcctgttcagcctgctttccgcgtttgcccccgatctgcaaatgctggtgttcctgcgttttctgaccggcctcggcctcggcggcgcaatgcctaataccatcaccatgacctcggaatatttgcctgcccggcggcgcggggcgctggtaaccctgatgttttgcgggttcaccctcggttccgccttcggcggcgtggtcagcgcacagctggtgccgctgatcggctggcacggcattctggtgctcggcggcgttttaccgttgatactggcggtggcgctggtgcctctgcttcccgagtctccgcgctggcagatccgtcgccagctgccgcaggcgacgatcgccagaaccgtcggcgccattaccggcgagcgctataacgacacgcatttctggcttgatgaacctgccgcaggggcgaagggcagtatcagccagctgttcgccgggcgtcagttggccatcaccttaatgctatgggtggtgttctttatgagcctgctgattatctatctgctctccagctggatgccgacgttgctaaaccatcggggcattgatttacagcacgcttcgtgggtcaccgccgccttccagattggcggcacctttggcgcgctgctgctcggcgtgctgatggatcgcttcaatccctatcgggtgctggcgctgagctacggcctcggggcggtgtgtatcgtcatgattggcctgagcgagaacggcctgtggctgatggcgctggcgattttcggcaccggcatcggcatcagcggctcgcaggtggggctgaatgcgctaaccgcaacgctgtatcccacgcaaagccgcgcgactggcgtgagctggtcaaacgcggttggccgctgcggggccatcgttggctccctttccggcggtgtgatgatggcgatgaatttctcattcgataccctgttttttgttatcgccgtcccggccgttgtgagcgccgtgatgcttatcctgctgacgcttgccgtccgcaacccgacttctgtacccgccgcgctgccttccgcaggcattgtgaataaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z