BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K2088668 1 BBa_K2088668 B0030+pbaC 2016-10-07T11:00:00Z 2016-10-19T12:20:46Z Klebsiella pneumoniaeM5a1,sequence found from genebank https://www.ncbi.nlm.nih.gov/nuccore/AY648560.1 3-hydroxybenzoate 6-hydroxylase fromKlebsiella pneumoniaeM5a1 is an enzyme that utilizes 3-hydroxybenzoate (3-HBA) as substrate yielding gentisate.3-HBA 6-hydroxylase introduces a new hydroxyl group at the para-position to the existing one and yields gentisate (2, 5-dihydroxybenzoate) , an intermediate step in the degradation of aromatic compounds in soil bacteria. false false _2556_ 29893 33447 9 false no false chenrui qin component2521882 1 BBa_K2088005 component2521876 1 BBa_B0030 annotation2521882 1 BBa_K2088005 range2521882 1 22 1385 annotation2521876 1 BBa_B0030 range2521876 1 1 15 BBa_K2088005 1 BBa_K2088005 pbaC with double Terminator 2016-10-08T11:00:00Z 2016-10-19T12:17:49Z This part is an optimization of BBa_K2088667. This part includes optimized mhbD using Codon Optimization Tool of iDT and a designed rbs using RBS Calculator. false false _2556_ 33751 33751 9 false An optimization of BBa_K2088667 with a designed rbs. false Xia Chen annotation2521810 1 ATG Start Codon range2521810 1 1 3 annotation2521813 1 pbaC coding sequence range2521813 1 1 1227 annotation2521811 1 TAG Stop Codon range2521811 1 1225 1227 annotation2521812 1 BBa_B0015 range2521812 1 1236 1364 BBa_B0030_sequence 1 attaaagaggagaaa BBa_K2088005_sequence 1 atgagctattatgacgttctgatcgtgggggccggccatgccggggcgcaagcagcgatttccctgcgccagcttgggttcgaaggatcggtcgcaatgatcggcgatgaaaaagacccgccctatgaacgtcccccgctgtccaaggaatatttcgcgggcgacaagaacttcgatcgcatcctcatcaggcccgccgccttctggggggaacggaaggtcgacatgctgctgggcaagcgcgtgaaacatgtcgatccgaccgccaaattcgtgacggcgggggatgaggaaatcggctatggttcgctgatctgggcgacgggcggcagtccgcgcatgttgacctgcaacggtgcggatgcgtccaacgtccatgcggtccgccgccgcgacgatgtcgacgccatgatggcgaagctggaccagatcgaccatgtgaccgtgatcggcggcggctatatcgggctggaggccgccgctgtcctcaccaagttcggcaagaaggtggtgctgctggaggcgctcgaccgggtactcgcccgcgtggcgggggaagatctttcgcgcttttacgaggccgagcatcgcgcccatggcgttgacctgcggaccggaacgaagatggactgcatcgaggtcagggacggcaaggcgaccgcggtgctgatgcaggacggcgagcggatcgagacagacatggttatcgtcggcatcggcatcgtgccggaaaccggccccctgatcgcggcgggcgccgctggcggcaatggtgtcgatgtggacgaatattgccgcaccagcctgccggatatctatgccgtaggcgattgcgcggcgcatgccaaccgctttgcccggggcgcgcaaattcgcctcgaatccgtgcagaacgccaatgatcaggcgaagacggcggtggcgcacatcatgggcaaggaagacccctatgacgcggtgccgtggttctggtccaatcaatatgacctgaaattgcagacggtcgggctgtcgaccggccatgaccagacgctattgcggggcgatccggcggcgcgcagcttttccgtgctatatttgaagggcgggaagctgatcgcgctagactgcgtcaatgcggtgaaggactatgtgcaggggcgcgcccatgtgctgtccggcgcggcgctggatcaggcgcaactggccgatccgtccgtgccgctcaaggaagtggggctggcttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2088668_sequence 1 attaaagaggagaaatactagatgagctattatgacgttctgatcgtgggggccggccatgccggggcgcaagcagcgatttccctgcgccagcttgggttcgaaggatcggtcgcaatgatcggcgatgaaaaagacccgccctatgaacgtcccccgctgtccaaggaatatttcgcgggcgacaagaacttcgatcgcatcctcatcaggcccgccgccttctggggggaacggaaggtcgacatgctgctgggcaagcgcgtgaaacatgtcgatccgaccgccaaattcgtgacggcgggggatgaggaaatcggctatggttcgctgatctgggcgacgggcggcagtccgcgcatgttgacctgcaacggtgcggatgcgtccaacgtccatgcggtccgccgccgcgacgatgtcgacgccatgatggcgaagctggaccagatcgaccatgtgaccgtgatcggcggcggctatatcgggctggaggccgccgctgtcctcaccaagttcggcaagaaggtggtgctgctggaggcgctcgaccgggtactcgcccgcgtggcgggggaagatctttcgcgcttttacgaggccgagcatcgcgcccatggcgttgacctgcggaccggaacgaagatggactgcatcgaggtcagggacggcaaggcgaccgcggtgctgatgcaggacggcgagcggatcgagacagacatggttatcgtcggcatcggcatcgtgccggaaaccggccccctgatcgcggcgggcgccgctggcggcaatggtgtcgatgtggacgaatattgccgcaccagcctgccggatatctatgccgtaggcgattgcgcggcgcatgccaaccgctttgcccggggcgcgcaaattcgcctcgaatccgtgcagaacgccaatgatcaggcgaagacggcggtggcgcacatcatgggcaaggaagacccctatgacgcggtgccgtggttctggtccaatcaatatgacctgaaattgcagacggtcgggctgtcgaccggccatgaccagacgctattgcggggcgatccggcggcgcgcagcttttccgtgctatatttgaagggcgggaagctgatcgcgctagactgcgtcaatgcggtgaaggactatgtgcaggggcgcgcccatgtgctgtccggcgcggcgctggatcaggcgcaactggccgatccgtccgtgccgctcaaggaagtggggctggcttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z