BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K2088671 1 BBa_K2088671 J23100+B0034+mhbT+dT 2016-10-08T11:00:00Z 2016-10-14T08:56:44Z This is a compostie part including a putative 3-hydroxybenzoate transporter gene(mhbT) from Klebsiella pneumoniae strain M5a1. This composite part includes a constitutive promoter (BBa_J23100), a RBS(BBa_B0034), a putative 3-hydroxybenzoate transporter gene(mhbT) and the double terminator (BBa_B0015). The gene MhbT is a transport protein which is located on the cytomembrane. It can transport the 3-HBA into the cell.By sequence comparison. This composite part can be used in degradation system of 3-HBA, and can also be used in induction system whose derivant is 3-HBA. false false _2556_ 33751 33723 9 false Uses J23100, a strong Anderson Promoter, to produce high levels of expression of mhbT. false Yuxin He component2488827 1 BBa_B0034 component2488831 1 BBa_K2088666 component2488838 1 BBa_B0015 component2488825 1 BBa_J23100 annotation2488825 1 BBa_J23100 range2488825 1 1 35 annotation2488831 1 BBa_K2088666 range2488831 1 62 1420 annotation2488838 1 BBa_B0015 range2488838 1 1429 1557 annotation2488827 1 BBa_B0034 range2488827 1 44 55 BBa_K2088666 1 T MhbT is responsible for the uptake of 3-hydroxybenzoate 2016-10-06T11:00:00Z 2016-10-08T03:01:00Z We get the part from the bacteria M5a1, and the sequence of the gene was found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/AY648560.1 MhbT is a transport protein which is located on the cytomembrane. It can transport the 3-HBA into the cell.By sequence comparison, MhbT exhibited 42% identity with the 4-hydroxybenzoate and protocatechuate transporter PcaK from P.putida, 27% identity with the benzoate transporter BenK from Acinetobacter sp. Using the MhbT-GFP fusion proteins , we can find that MhbT is located to the periphery of cells. It can be used in degradation system of 3-HBA, and can also be used in induction system whose derivant is 3-HBA. false false _2556_ 29893 29893 9 false None false Shi Kejian annotation2486847 1 MhbT coding region range2486847 1 1 1359 annotation2486846 1 TAA stop codon range2486846 1 1357 1359 annotation2486845 1 ATG start codon range2486845 1 1 3 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K2088671_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgactcaacgacttgaattacagaagctgctcaacgccgcgccggtcggcgcccgacagtggcgcgtgattatctgctgtttcctggtggtgatgctcgatggctttgataccgccgcgattggttttatcgcgccggatatccgcagccactggcagctcaccgccggcgacctcgcgccgttatttggcgcgggattgctgggcctgaccgccggggcgctgctctgcggcccgctctccgaccgctttggccgcaaacgggtgattgagttttgcgtcgccctgttcggcctgttcagcctgctttccgcgtttgcccccgatctgcaaatgctggtgttcctgcgttttctgaccggcctcggcctcggcggcgcaatgcctaataccatcaccatgacctcggaatatttgcctgcccggcggcgcggggcgctggtaaccctgatgttttgcgggttcaccctcggttccgccttcggcggcgtggtcagcgcacagctggtgccgctgatcggctggcacggcattctggtgctcggcggcgttttaccgttgatactggcggtggcgctggtgcctctgcttcccgagtctccgcgctggcagatccgtcgccagctgccgcaggcgacgatcgccagaaccgtcggcgccattaccggcgagcgctataacgacacgcatttctggcttgatgaacctgccgcaggggcgaagggcagtatcagccagctgttcgccgggcgtcagttggccatcaccttaatgctatgggtggtgttctttatgagcctgctgattatctatctgctctccagctggatgccgacgttgctaaaccatcggggcattgatttacagcacgcttcgtgggtcaccgccgccttccagattggcggcacctttggcgcgctgctgctcggcgtgctgatggatcgcttcaatccctatcgggtgctggcgctgagctacggcctcggggcggtgtgtatcgtcatgattggcctgagcgagaacggcctgtggctgatggcgctggcgattttcggcaccggcatcggcatcagcggctcgcaggtggggctgaatgcgctaaccgcaacgctgtatcccacgcaaagccgcgcgactggcgtgagctggtcaaacgcggttggccgctgcggggccatcgttggctccctttccggcggtgtgatgatggcgatgaatttctcattcgataccctgttttttgttatcgccgtcccggccgttgtgagcgccgtgatgcttatcctgctgacgcttgccgtccgcaacccgacttctgtacccgccgcgctgccttccgcaggcattgtgaataaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2088666_sequence 1 atgactcaacgacttgaattacagaagctgctcaacgccgcgccggtcggcgcccgacagtggcgcgtgattatctgctgtttcctggtggtgatgctcgatggctttgataccgccgcgattggttttatcgcgccggatatccgcagccactggcagctcaccgccggcgacctcgcgccgttatttggcgcgggattgctgggcctgaccgccggggcgctgctctgcggcccgctctccgaccgctttggccgcaaacgggtgattgagttttgcgtcgccctgttcggcctgttcagcctgctttccgcgtttgcccccgatctgcaaatgctggtgttcctgcgttttctgaccggcctcggcctcggcggcgcaatgcctaataccatcaccatgacctcggaatatttgcctgcccggcggcgcggggcgctggtaaccctgatgttttgcgggttcaccctcggttccgccttcggcggcgtggtcagcgcacagctggtgccgctgatcggctggcacggcattctggtgctcggcggcgttttaccgttgatactggcggtggcgctggtgcctctgcttcccgagtctccgcgctggcagatccgtcgccagctgccgcaggcgacgatcgccagaaccgtcggcgccattaccggcgagcgctataacgacacgcatttctggcttgatgaacctgccgcaggggcgaagggcagtatcagccagctgttcgccgggcgtcagttggccatcaccttaatgctatgggtggtgttctttatgagcctgctgattatctatctgctctccagctggatgccgacgttgctaaaccatcggggcattgatttacagcacgcttcgtgggtcaccgccgccttccagattggcggcacctttggcgcgctgctgctcggcgtgctgatggatcgcttcaatccctatcgggtgctggcgctgagctacggcctcggggcggtgtgtatcgtcatgattggcctgagcgagaacggcctgtggctgatggcgctggcgattttcggcaccggcatcggcatcagcggctcgcaggtggggctgaatgcgctaaccgcaacgctgtatcccacgcaaagccgcgcgactggcgtgagctggtcaaacgcggttggccgctgcggggccatcgttggctccctttccggcggtgtgatgatggcgatgaatttctcattcgataccctgttttttgttatcgccgtcccggccgttgtgagcgccgtgatgcttatcctgctgacgcttgccgtccgcaacccgacttctgtacccgccgcgctgccttccgcaggcattgtgaataaataa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z