LOCUS BBa_K2088672 4093 bp DNA linear 05-Nov-2024 DEFINITION copA + riboJ +mhbDHIM + terminator ACCESSION BBa_K2088672 VERSION BBa_K2088672.1 FEATURES Location/Qualifiers promoter 1..242 /label=BBa_K2088001 misc_feature 243..323 /label=BBa_K2066506 RBS 324..348 /label=native rbs of mhbD CDS 349..1386 /label=mhbD RBS 1387..1410 /label=native rbs of mhbH CDS 1411..2109 /label=mhbH RBS 2110..2117 /label=native rbs of mhbI CDS 2118..2762 /label=mhbI RBS 2763..2770 /label=native rbs of mhbM CDS 2771..3964 /label=mhbM misc_feature 324..3964 /label=BBa_K2088692 stem_loop 3976..4019 /label=stem_loop misc_feature 3965..4044 /label=BBa_B0010 terminator 3965..4044 /label=BBa_B0010 stem_loop 4060..4079 /label=T7 TE misc_feature 4086..4086 /label=stop polyA_site 4080..4093 /label=polya misc_feature 4053..4093 /label=BBa_B0012 terminator 4053..4093 /label=BBa_B0012 terminator 3965..4093 /label=BBa_B0015 ORIGIN 1 cctttttata gatgcgggag gtaattcctc accccggtgc cgattttcag gcatcctgat 61 ttaacttagc acccgcaact taactacagg aaaacaaaga gataaatgtc taatcctgat 121 gcaaatcgag ccgatttttt aatctttacg gacttttacc cgcctggttt attaatttct 181 tgaccttccc cttgctggaa ggtttaacct ttatcacagc cagtcaaaac tgtcttaaag 241 gtagctgtca ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta 301 caaataattt tgtttaaact agattgtgaa taaataagga gaacgcttat gtcccagtcc 361 accacggaag caaataacgg ccgtcagcag ttttaccagc atatttccgg gcaaaacctg 421 acgccgcttt gggaatcgct gcaccatctg gtgccgaaaa cgccgaacgc cacctgcgcg 481 ccggcttact ggaattatca ggagattcgc ccgctgctgc tggaaagcgg caagctgatt 541 ggcgcgaaag aggcgatacg ccgcgtgctg gtgctggaaa acccggcgct gcgcgggcag 601 tcgtcgatta cctcctcgct gtacgcgggt ttgcagctga ttatgcccgg cgaagtggcg 661 ccgagccatc ggcacaacca gtcggcgctg cgctttgtcg tggaaggcga aggggcgttt 721 accgccgttg acggcgaacg gaccgccatg cgcgccggcg actttattct gacgccgcag 781 tggcgctggc acgatcacgg caaccccggc aacgaaccgg ttatctggct cgacggcctc 841 gatctgccgc tggtgaacta tctgggctgc ggcttcgcgg aggactatcc gcaagatcag 901 cagccggtga cccgcaaaga gggggattat ctgccgcgct acgccgccaa catgctgccg 961 ctgcgccatc agtccggtaa ctcttcgccc atctttaact atcgctacga ccgcagccgc 1021 gaagcgctgc acgatctgac gcggatgggc gatgccgacg agtgggatgg ctataagatg 1081 cgctacgtca acccggtcac cggcggctac ccgatgccgt cgatgggcgc cttcctgcaa 1141 ctgttaccga aagggtttac ctcgcgagcg gcgaaaacca ccgacagtac cgtctatcac 1201 gtggtggaag gcagcggcca ggtcaccatt ggcgaacaga ccttcgcttt tcaggcaaaa 1261 gatatcttcg tggtgccgac ctggcacgcc gtctctttta tctccgccga agatagcgtg 1321 ttattcagct tttcggaccg tcccgtgcag gaagcgctcg gcctgttccg cgaagcgcgt 1381 tattaaaaat taagaataag gaatcgaatc atgactcaat acgtttttgc accccaggct 1441 ccgattagcg ttccggttgt cggcagcgat gagcagttcc cggtgcgccg cgtttactgc 1501 gtgggccgca actacgccgc tcacgcgcgt gaaatgggct ttgacccgga tcgcgaaccg 1561 ccgttcttct tctgcaagcc tgccgatgcg gtggtgcccg tcgccgcggg agaaacgctg 1621 aacctgccgt atccgacgca aaccgataac tatcactacg aaattgagct ggtggtggcg 1681 attggcaagc ggggaagcga tatcccgctg gagcaggccc atgagtatat ctggggctat 1741 gccacggggc tggatatgac ccgccgcgat cgccagatgg agatgcgcca gatgggccgt 1801 ccgtgggaga ttggtaaagc ttttgacctt tccgcgccga ttgcgccgct gcataaagcg 1861 gatacggcgg acgtgagcca ggccggaatc tggctgcaag taaacgggga agatcatcag 1921 cgcagcgata ttcgccacct gatctggtcg gttaacgaaa ccatcagcta tctttccggt 1981 ttctttgagc tgcaacccgg cgatctgatt ttcaccggca cgccggaagg cgtcggcgcg 2041 gtggtgaaag gcgatgtcat caccggcaac gtcgatggac tgacgccaat cgccgtgcgc 2101 gtggtgtgag gtgaatgatg aagctgtaca gtttttttaa tagctcagcg tcctaccgcg 2161 tgcggattgc gctggcgctg aaaggcatcg actaccagag cgtcggcgtt aacatccgca 2221 tcggccagca gaacgcgctg gagtaccggc ggctgaaccc ggtcggtctg gtgccgacgc 2281 tcattaccga caaaggcgaa tctctcggcc agtcgctggc gattgccgac tggctggacc 2341 ggcactatcc gcagccgctg ctgctgcccc aggcggacag cgcgcggatg cgggtactgg 2401 agatcgttta cgccatcgcc tgcgatattc atcccattaa taacatgcgc gttttacgct 2461 accttggcga cgagctaaag gtaagcgaag aggagaaaaa acgctggtat gcgcactgga 2521 tccagcaggg attcagcgcc gtcgagcagc tattgcgcca cgcgaaatcc ggcgattttt 2581 gcgtgggcga tgcgcctacc ctggcggact gctgcctggt gccgcagtgg gccaacgccc 2641 tgaggatggg ctgcgatctc agccactatc cgcgctgcca ggcggtttat gacgcctgta 2701 cccggctgcc ggcatttatc gccgccgcgc cggaaaacca acaagataag atcccggctt 2761 aggaggcaac atggcaaaag tgatgcgagc aattattgtc ggcggcggta ttggcggagc 2821 ggccacggcg ctctctctgg cgcgccaggg catcaaagtg atgctgctgg aaaaggcgca 2881 tgaaatcggc gaaatcggcg ccgggatcca gttgggtcca aacgcattct cggcgctcga 2941 cagcctcggc gtcggcgaag tcgcccgcca gcgcgcggtg tttaccgacc atatcaccat 3001 gatggatgcg gtcaacggcg aagaggtggt gcatattgag accggacagg ccttccgcga 3061 ccattttggc ggaccctatg cggtgatcca tcgggtggat atccacgcca ccgtctggga 3121 ggcggcgctg acccatccgg ccgtcgagta ccgtacttcg acgcaggtag tggatatccg 3181 ccagaccgcc gatgacgtga cggtgtttga tgataaaggc aacagctgga cggcggatat 3241 tctgatcggc tgcgacggcg gtaagtcggt ggtgcgccaa agtctgctgg gcgattcgcc 3301 gcgggtaacc ggccatgtcg tttatcgcgc ggtggttgac gccgccgata tgccggacga 3361 tttacgcatc aacgcgccgg tgctgtgggc cggtccgcac tgccacctgg tgcattatcc 3421 gctgcgcggc ggcaagcagt acaacctggt ggtgaccttc catagtcggc agcaggaaga 3481 gtggggcgtg cgcgacggca gtaaagaaga ggtgctctcc tactttaaag gcatccaccc 3541 gcgtccccgg cagatgctgg acaaaccgac ctcctggcgt cgctggtcca ccgccgaccg 3601 cgagccggta gagaagtggg gcaacgaccg catcacgctg gttggcgatg ccgcgcatcc 3661 ggtggcgcag tatatggccc agggcgcctg tatggcgctg gaagatgcgg tgacgctggg 3721 taaagcgctg gcgcaatgcg acggcgacgc cgcccgcgct ttcgcgctgt atgaatcggt 3781 acgtattccg cgtaccgcgc gcatcgtctg gtcgacgcgg gaaatggggc gggtctatca 3841 cgccgccggc gttgagcgtc aggtgcgcaa tctgctgtgg aaaggcaaaa cccagagcga 3901 gttttatcgc ggcatcgagt ggctgtacgg ctggaaagag gataactgcc tggaagcgcg 3961 ttaaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 4021 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 4081 ttctgcgttt ata //